Molecular sieving polymer for DNA/RNA separation in capillary electrophoresis

In capillary polymer electrophoresis, the property of polymer sieving matrix dominates the migration behavior of DNA/RNA. We investigated the capillary electrophoresis of RNA ranging from 100 nt to 10,000 nt in polyacrylamide (PA) solutions with different molecular weights (Mw) and different concentrations. We observed that the resolution length (RSL) of RNA fragments was improved and the migration time was prolonged, when polymer concentration was increased. The resolution for small RNA fragments ([Formula: see text]1000 nt) was improved with the increase of polymer concentration, whereas the large ones ([Formula: see text]3000 nt) became inseparable. In addition, we estimated the smallest resolvable nucleotide length (Ls) by the plot of RSL against RNA size.

Download Full-text

Effect of Asparagus Polysaccharides on Migration Time of Erythrocytes in Tumor-Bearing Organisms Using High Performance Capillary Electrophoresis

2008 2nd International Conference on Bioinformatics and Biomedical Engineering ◽

10.1109/icbbe.2008.904 ◽

2008 ◽

Author(s):

Lang Lang ◽

Yu-Bin Ji ◽

Chen-Feng Ji ◽

Xiang Zou ◽

Lei Yu

Keyword(s):

Capillary Electrophoresis ◽

High Performance ◽

Migration Time ◽

Tumor Bearing ◽

High Performance Capillary Electrophoresis

Download Full-text

Controllable Molecular Sieving by copoly(Poly(ethylene glycol) Acrylate/Poly(ethylene glycol) Diacrylate)-Based Hydrogels via Capillary Electrophoresis for DNA Fragments

ACS Applied Polymer Materials ◽

10.1021/acsapm.0c00567 ◽

2020 ◽

Vol 2 (9) ◽

pp. 3886-3893

Author(s):

Chenchen Liu ◽

Takuya Kubo ◽

Toyohiro Naito ◽

Koji Otsuka

Keyword(s):

Capillary Electrophoresis ◽

Ethylene Glycol ◽

Dna Fragments ◽

Poly Ethylene Glycol ◽

Glycol Diacrylate ◽

Poly Ethylene ◽

Molecular Sieving

Download Full-text

Quantitative description of analyte migration behavior based on dynamic complexation in capillary electrophoresis with one or more additives

Electrophoresis ◽

10.1002/elps.1150180509 ◽

1997 ◽

Vol 18 (5) ◽

pp. 706-716 ◽

Cited By ~ 47

Author(s):

Xuejun Peng ◽

Michael T. Bowser ◽

Philip Britz-McKibbin ◽

Gwendolyn M. Bebault ◽

Jamie R. Morris ◽

...

Keyword(s):

Capillary Electrophoresis ◽

Quantitative Description ◽

Migration Behavior ◽

Analyte Migration ◽

Behavior Based

Download Full-text

Towards adaptive method for peak migration time correction: discretization period in electropherograms

Chemija ◽

10.6001/chemija.v31i3.4288 ◽

2020 ◽

Vol 31 (3) ◽

Author(s):

Tomas Drevinskas ◽

Audrius Maruška ◽

Gintarė Naujokaitytė ◽

Laimutis Telksnys ◽

Mihkel Kaljurand ◽

...

Keyword(s):

Capillary Electrophoresis ◽

Adaptive Method ◽

Reference Points ◽

Migration Time ◽

Time Shift ◽

Internal Standards ◽

Relative Standard ◽

Adaptive Peak ◽

Time Correction ◽

Computationally Intensive

Capillary electrophoresis often causes unrepeatable peak migration times in the electropherogram due to changes of electroosmosis, yet in some cases this separation technique does not have a replacement alternative. Some attempts to overcome this issue have been performed introducing internal standards into the sample and compensating peak shifting in time. However, existing vector calculation-based methods are computationally intensive for portable instrumentation and usually limited to post-processing applications with 1 or 2 markers. In this work, an original approach of compensating peak migration time shift via signal discretization period correction is proposed. Using the proposed method, the number of reference points or markers that are used for compensation is extended. This method is effective in compensating migration time of peaks in real samples, where high sample injection volumes are used. Using 4 reference peaks in compensation, the method was capable of reducing the relative standard deviation of migration time of the peaks in the electropherograms more than 15 times. Corrected signal discretization periods indicated very high correlations with recorded separation currents, what can be perspective developing an adaptive peak migration time compensation method in capillary electrophoresis.

Download Full-text