Chronic allergy to dietary ovalbumin induces lymphocyte migration to rat small intestinal mucosa that is inhibited by MAdCAM-1

2004 ◽  
Vol 286 (5) ◽  
pp. G702-G710 ◽  
Author(s):  
Toshiko Ogawa ◽  
Soichiro Miura ◽  
Yoshikazu Tsuzuki ◽  
Takashi Ogino ◽  
Ken Teramoto ◽  
...  
Keyword(s):  
Food Allergy ◽  
T Lymphocytes ◽  
Intestinal Mucosa ◽  
Morphological Changes ◽  
Peyer's Patches ◽  
Brown Norway ◽  
Peyer’S Patches ◽  
Small Intestinal Mucosa ◽  
Lymphocyte Migration ◽  
Small Intestinal

Few models have described a chronic food allergy with morphological changes in the intestinal mucosa. Here we established an ovalbumin (OVA)-induced, cell-mediated, allergic rat model and examined lymphocyte migration in the gut. Brown Norway rats were intraperitoneally sensitized to OVA and then given 10 mg OVA/day by gastric intubation for 6 wk. Lymphocyte subsets and adhesion molecules were examined immunohistochemically, and the migration of T lymphocytes to microvessels of Peyer's patches and villus mucosa was observed by using an intravital microscope. Serum OVA-specific IgG and IgE levels were increased in animals repeatedly exposed to OVA. Significant villus atrophy and increased crypt depth was accompanied by increased infiltration of T lymphocytes in the small intestinal mucosa of the group given OVA. Expression of rat mast cell protease II and of mucosal addressin cell adhesion molecule-1 (MAdCAM-1) was also increased in these groups. The administration of anti-MAdCAM-1 antibody significantly attenuated the OVA-induced changes in the mucosal architecture and in CD4 T lymphocyte infiltration. Intravital observation demonstrated that in rats with a chronic allergy, T lymphocytes significantly accumulated in villus microvessels as well as in Peyer's patches via a MAdCAM-1-dependent process. Our model of chronic food allergy revealed that lymphocyte migration was increased with MAdCAM-1 upregulation.

scholarly journals Human small intestinal mucosa harbours a small population of cytolytically active CD8+ alphabeta T lymphocytes

Immunology ◽  
2002 ◽  
Vol 106 (4) ◽  
pp. 476-485 ◽  
Author(s):  
Silvia Melgar ◽  
Anna Bas ◽  
Sten Hammarstrom ◽  
Marie-Louise Hammarstrom
Keyword(s):  
T Lymphocytes ◽  
Intestinal Mucosa ◽  
Small Population ◽  
Small Intestinal Mucosa ◽  
Small Intestinal

Uptake of Mycobacterium avium subsp. paratuberculosis through the Distal Small Intestinal Mucosa in Goats: An Ultrastructural Study

2001 ◽  
Vol 38 (2) ◽  
pp. 184-189 ◽  
Author(s):  
Ó. G. Sigurðardóttir ◽  
C. M. Press ◽  
Ø. Evensen
Keyword(s):  
Mycobacterium Avium ◽  
Intestinal Wall ◽  
Peyer's Patches ◽  
Mycobacterium Avium Subsp ◽  
Peyer’S Patches ◽  
M Cells ◽  
Small Intestinal Mucosa ◽  
Intestinal Segments ◽  
Small Intestinal ◽  
Mycobacterium Avium Subsp Paratuberculosis

Various pathogens gain access to the intestinal wall via specialized cells, the M cells, found among the follicle-associated epithelial cells overlying the domes of the Peyer's patches. The present study was undertaken to examine the uptake of live Mycobacterium avium subsp. paratuberculosis in the distal small intestine of goat kids. Following laparotomy, distal small intestinal segments of five goats were ligated and injected with bacterial suspension. After 1 hour, the intestinal segments were excised and fixed for light and electron microscopic studies. M. a. paratuberculosis organisms were observed by transmission electron microscopy at locations in the intestinal wall, suggesting transcellular transportation through the M cells. The organisms were present both in the cytoplasm of the M cells and in the cytoplasm of intraepithelial leukocytes found in M-cell pockets. Intercellular bacteria between M cells were occasionally seen. Bacteria were not observed in association with the absorptive epithelium. This study indicates that in goat kids, M. a. paratuberculosis enters the intestinal wall primarily through the M cells in the follicle-associated epithelium of the Peyer's patches.

Endotoxin stimulates lymphocyte-endothelial interactions in rat intestinal Peyer's patches and villus mucosa

1996 ◽  
Vol 271 (2) ◽  
pp. G282-G292 ◽  
Author(s):  
S. Miura ◽  
Y. Tsuzuki ◽  
I. Kurose ◽  
M. Suematsu ◽  
T. Shigematsu ◽  
...  
Keyword(s):  
Endothelial Cell ◽  
Shear Rate ◽  
T Lymphocytes ◽  
Intestinal Mucosa ◽  
Cell Interactions ◽  
Peyer's Patches ◽  
Peyer’S Patches ◽  
Significant Heterogeneity ◽  
Adhesive Interaction ◽  
Beta 2

Although lymphocyte-endothelial cell interactions represent a key step in controlling the recruitment of lymphocytes into gut-associated tissues, its dynamic process in microvessels of lymphoid (Peyer's patches) and nonlymphoid (villus) regions of the small bowel remains poorly understood. We monitored the migration of fluorescence-labeled T lymphocytes into normal and lipopolysaccharide (LPS)-inflamed rat intestinal microvessels using intravital microscopy. In Peyer's patches, T lymphocytes selectively adhered to postcapillary venules, although such selectivity was not observed in submucosal venules of villi. T lymphocytes exhibited rolling behavior followed by firm adhesion in microvessels of both the Peyer's patches and the villi, with both types of adhesive interaction being mediated by alpha 4-integrins. The enhanced rolling and adherence of lymphocytes observed in Peyer's patches and submucosal venules of villi of LPS-treated rats were preceded by a reduction in shear rate and were mediated largely by alpha 4-integrins and partly by beta 2-integrins. In capillaries of intestinal mucosa, lymphocyte adherence occurred without rolling and was independent of alpha 4-integrins. LPS also significantly increased adherence of lymphocytes to villus capillaries, which was not mediated by either alpha 4- or beta 2-integrin. These observations demonstrate significant heterogeneity of lymphocyte-endothelial cell interactions within different regions of the intestinal mucosa.

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