Effects of sulfobromophthalein excretion on biliary lipid secretion in humans and dogs

The effect of sulfobromophthalein (BSP) on biliary lipid secretion was investigated in 12 cholecystectomized subjects, using a duodenal marker-perfusion technique. A 1-h basal period was followed by intravenous BSP infusion over 3 h, achieving the maximal excretory rate (Tm). The calculated Tm was not different from the measured maximal output. At BSP Tm, bile salt secretion was unchanged, but phospholipid, cholesterol, and bilirubin secretion were markedly reduced. Biliary lipid composition changed accordingly, higher molar percent bile salts but lower phospholipid and cholesterol. In six cholecystectomized dogs with chronic duodenal fistulas, bile was collected directly from the common duct while bile salt secretion was maintained by intravenous taurocholic acid infusion. After a 2-h control period, sufficient BSP was added to create either maximal (Tm) or submaximal conditions. BSP did not alter bile salt secretion but caused a dose-related decrease in phospholipid and cholesterol secretion. Bilirubin excretion was also reduced, whereas bile flow increased. Thus, BSP is hydrocholeretic but decreases phospholipid, cholesterol, and bilirubin secretion in both humans and dogs. The effect on biliary lipid composition is probably through a physical interaction with biliary micelles.

Download Full-text

Control of biliary phospholipid secretion. Effect of continuous and discontinuous infusion of taurocholate on biliary phospholipid secretion

Biochemical Journal ◽

10.1042/bj2340421 ◽

1986 ◽

Vol 234 (2) ◽

pp. 421-427 ◽

Cited By ~ 17

Author(s):

K Rahman ◽

T G Hammond ◽

P J Lowe ◽

S G Barnwell ◽

B Clark ◽

...

Keyword(s):

Animal Model ◽

Bile Salt ◽

Biliary Fistula ◽

Perfused Liver ◽

Biliary Lipid ◽

Short Pulses ◽

Lipid Secretion ◽

Canalicular Membrane ◽

Infusion Period ◽

Salt Secretion

A major determinant of biliary lipid secretion is bile-salt secretion. Taurocholate (TC), a micelle-forming bile salt, was infused continuously at different rates in both isolated perfused livers and biliary-fistula rats. In both of these systems, infusion of TC brought about an elevated secretion of phosphatidylcholine for the duration of the TC infusion period. Initial phospholipid/bile-salt ratios in the bile were higher in the whole-animal model than in isolated livers, but at the higher infusion rates both secreted approx. 6 mol of phospholipid for every 100 mol of bile salt. The secretion of phospholipid, which was maintained even at high rates of bile-salt infusion, suggest a continuous and regulated phospholipid supply and secretion mechanism. In contrast, however, multiple short pulses of TC to the perfused liver, which brought about relatively equal biliary bile-salt output pulses, did not bring about equal phospholipid outputs, since the phospholipid peak size declined with each bile-salt pulse. These experiments taken together suggest either that a threshold (intracellular) bile-salt concentration may be required to ‘switch-on’ the phospholipid supply and that it may need to be maintained for continuous biliary phospholipid supply to the canalicular membrane.

Download Full-text

Selective biliary lipid secretion at low bile-salt-output rates in the isolated perfused rat liver. Effects of phalloidin

Biochemical Journal ◽

10.1042/bj2370301 ◽

1986 ◽

Vol 237 (1) ◽

pp. 301-304 ◽

Cited By ~ 10

Author(s):

K Rahman ◽

R Coleman

Keyword(s):

Bile Salt ◽

Bile Salts ◽

Isolated Perfused Rat Liver ◽

Perfused Rat Liver ◽

Biliary Lipid ◽

Perfusion Fluid ◽

Lipid Secretion ◽

Rat Livers ◽

Secretion Rates ◽

Salt Secretion

At high bile-salt-secretion rates the biliary secretion of phospholipids and cholesterol is dependent on that of the bile salts. However, at low bile-salt outputs some secretion remains. Isolated perfused rat livers were used in these experiments in order to study the bile-salt-independent secretion of biliary lipids. The livers were isolated and saline (0.9% NaCl), or phalloidin dissolved in saline, was added to the perfusion fluid after 1 h of liver isolation. The concentration and output of cholesterol was significantly decreased in phalloidin-treated livers compared with the controls, whereas there was no significant decrease in phospholipids; the secretion of cholesterol and phospholipids can thus be uncoupled from each other by the action of phalloidin. These experiments suggest that a proportion of cholesterol gets into bile independently of bile salts and phospholipids. These findings are discussed in relation to the supersaturation of some biles with cholesterol and its relationship to the bile-salt-independent fraction of cholesterol.

Download Full-text

Effects of the fibre components pectin, cellulose, and lignin on bile salt metabolism and biliary lipid composition in man.

Gut ◽

10.1136/gut.27.1.29 ◽

1986 ◽

Vol 27 (1) ◽

pp. 29-36 ◽

Cited By ~ 39

Author(s):

L C Hillman ◽

S G Peters ◽

C A Fisher ◽

E W Pomare

Keyword(s):

Bile Salt ◽

Lipid Composition ◽

Biliary Lipid ◽

Biliary Lipid Composition

Download Full-text

Effects of organic anions on biliary lipid secretion in rats. Importance of association with biliary lipid structures

Biochemical Journal ◽

10.1042/bj2860193 ◽

1992 ◽

Vol 286 (1) ◽

pp. 193-196 ◽

Cited By ~ 6

Author(s):

G Yamashita ◽

S Tazuma ◽

G Kajiyama

Keyword(s):

Bile Salt ◽

Organic Anion ◽

Sodium Taurocholate ◽

Organic Anions ◽

Phenol Red ◽

Dependent Manner ◽

Biliary Lipid ◽

Lipid Secretion ◽

Biliary Lipids ◽

Salt Secretion

This study was performed to determine the effects of various organic anions on biliary lipid secretion in rats. We infused bile-salt-pool-depleted rats with sodium taurocholate at a constant rate, with or without various organic anions: Indocyanine Green (ICG), bromosulphophthalein (BSP), BSP-glutathione and Phenol Red (PR). BSP decreased biliary secretion of cholesterol and phospholipids in a dose-dependent manner without affecting bile salt secretion (uncoupling), and this change was fully reversible. In contrast, ICG, BSP-glutathione and PR did not cause such an uncoupling of biliary lipids. In addition, the distribution pattern of each organic anion to various lipid particles was determined by gel-permeation chromatography. BSP was predominantly associated with bile salt micelles, whereas vesicular association was dominant for ICG, and both BSP-glutathione and PR formed only self-aggregations. From these data, we concluded that the uncoupling of biliary lipids from bile salt secretion by BSP resulted from the interaction between BSP and bile salt micelles in the bile canaliculus, and that this interaction inhibited the capacity of bile salts to induce the secretion of phospholipids and cholesterol.

Download Full-text