Attenuation of endotoxin-induced intestinal microcirculatory damage by eicosapentanoic acid

1993 ◽  
Vol 264 (5) ◽  
pp. G828-G834 ◽  
Author(s):  
S. Miura ◽  
H. Imaeda ◽  
H. Shiozaki ◽  
I. Kurose ◽  
D. Fukumura ◽  
...  
Keyword(s):  
High Speed ◽  
Platelet Activating Factor ◽  
Video Camera ◽  
Ileal Mucosa ◽  
Camera System ◽  
Endotoxin Infusion ◽  
Eicosapentanoic Acid ◽  
High Speed Video Camera ◽  
Male Wistar Rats ◽  
Video Camera System

The major objective of this study is to investigate whether oral administration of eicosapentanoic acid (EPA) has any preventive effect on endotoxin-induced microcirculatory damage of rat small intestine. EPA in a daily dose of 300 mg/kg was orally given to male Wistar rats for 3 wk. Submucosal microvessels of the ileum were observed by intravital microscopy equipped with a high-speed video camera system after the intra-arterial infusion of endotoxin at a dose of 2 mg.kg-1.h-1. The number of sticking leukocytes was significantly increased at 30 min after the treatment of endotoxin especially along the smaller branch of intestinal venules. It reached the maximal plateau at 45 min after treatment. The pretreatment of EPA significantly attenuated the increase in sticking leukocytes induced by endotoxin. A platelet-activating factor (PAF) antagonist 2-[N-acetyl-N-(2-methoxy-3-octadecylcarbamoyloxy propoxycarbonyl) aminomethyl]-1-ethylpyridinium chloride (CV-6209) significantly prevented the increased leukocyte sticking to the same extent as EPA treatment. Thirty minutes after endotoxin infusion, red blood cell (RBC) velocity was significantly decreased in both arterioles and venules. RBC velocity appeared to be continuously decreased thereafter and reached its minimum value at approximately 60 min. EPA treatment was revealed to prevent the decrease in RBC velocity of microvessels induced by endotoxin. CV-6209 also significantly attenuated the decreased RBC velocity. The remarkable elevation of PAF content in the ileal mucosa as observed by endotoxin infusion was also significantly attenuated by administration of EPA.(ABSTRACT TRUNCATED AT 250 WORDS)

Vibration Characteristics of Magnetic Fluid Droplet Adsorbed to Magnetized Needlepoint in Alternating Magnetic Field

2012 ◽  
Vol 721 ◽  
pp. 108-113
Author(s):  
Sota Inomata ◽  
Seiichi Sudo ◽  
Hidemasa Takana ◽  
Hideya Nishiyama
Keyword(s):  
Magnetic Field ◽  
External Magnetic Field ◽  
Magnetic Fluid ◽  
High Speed ◽  
Video Camera ◽  
Alternating Magnetic Field ◽  
Camera System ◽  
High Speed Video ◽  
High Speed Video Camera ◽  
Video Camera System

The dynamic behavior of a magnetic fluid droplet adsorbed to magnetized needlepoint in alternating magnetic field was studied with a high speed video camera system. The directions of alternating magnetic field were parallel and opposite to static magnetic field of magnetized needlepoint. It was found that the surface of magnetic fluid droplet responds to the external magnetic field in elongation and contraction. The frequency of magnetic fluid droplet oscillation was exactly same of the external magnetic field. The shape and instability oscillations of the magnetic fluid droplet were revealed experimentally.

Measurement of Spraying Particle Behaviors by Three-Intensified-Sensor Color High Speed Video Camera System

1998 ◽  
Author(s):  
M. Yamakawa ◽  
S. Oki ◽  
S. Gohda
Keyword(s):  
High Speed ◽  
Optical Filter ◽  
Video Camera ◽  
Image Sensors ◽  
Camera System ◽  
Spray Coatings ◽  
High Speed Video ◽  
Simultaneous Measurements ◽  
High Speed Video Camera ◽  
Video Camera System

Abstract Three-intensified-sensor color high speed video camera system with a cubic three-way beam-split prism and three optical filter holders has been developed. One of three image sensors can be assigned for the measurement of velocity and the others for that of temperature. Simultaneous measurements of temperature and velocity of sprayed particles are tried with this camera for understanding of the formation mechanism of spray coatings. The possibility of measuring the velocity of individual particle in plasma spraying is discussed for behavior analysis of sprayed particles with three-intensified- sensor color high speed video camera system. Furthermore the possibility for simultaneous measurements of temperature and velocity of individual sprayed particles is examined.

Measurement of RBC Velocities in the Rat Pial Arteries with an Image-Intensified High-Speed Video Camera System

1998 ◽  
Vol 56 (3) ◽  
pp. 166-172 ◽  
Author(s):  
Mami Ishikawa ◽  
Eiichi Sekizuka ◽  
Katsuyoshi Shimizu ◽  
Noriyuki Yamaguchi ◽  
Takeshi Kawase
Keyword(s):  
High Speed ◽  
Video Camera ◽  
Pial Arteries ◽  
Camera System ◽  
High Speed Video ◽  
High Speed Video Camera ◽  
Video Camera System

Roles of ET-1 in endotoxin-induced microcirculatory disturbance in rat small intestine

1996 ◽  
Vol 271 (3) ◽  
pp. G461-G469 ◽  
Author(s):  
S. Miura ◽  
D. Fukumura ◽  
I. Kurose ◽  
H. Higuchi ◽  
H. Kimura ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Intestinal Mucosa ◽  
High Speed ◽  
Platelet Activating Factor ◽  
Video Camera ◽  
Mucosal Damage ◽  
Dependent Manner ◽  
Camera System ◽  
Eta Receptor Antagonist ◽  
Leukocyte Sticking

The major objective of this study was to investigate whether endothelin-1 (ET-1) plays a significant role in endotoxin-induced microcirculatory disturbances of the intestinal mucosa. Submucosal microvessels of the rat ileum were observed by intravital microscopy with a high-speed video camera system. Preceding the apparent intestinal mucosal damage, red blood cell (RBC) velocity was significantly decreased 30 min after endotoxin treatment in both arterioles and venules. The number of leukocytes sticking to submucosal venules was significantly increased at 30 min. BQ-123, an ETA-receptor antagonist, significantly attenuated the decrease in RBC velocity and also prevented an increase in leukocyte sticking as well as the subsequent mucosal damage induced by endotoxin. The ET-1 concentrations began to be elevated in plasma at 15 min and in the mucosa at 30 min and subsequently further increased in a time-dependent manner. A significant decrease in calcium-dependent nitric oxide synthase activity and significant increases in the concentration of platelet-activating factor (PAF) were demonstrated in the intestinal mucosa after endotoxin treatment. BQ-123 also significantly attenuated these changes. We concluded that the increased ET-1 production in intestinal mucosa induced by endotoxin stimulation could lead to leukocyte sticking and decreased RBC velocity in the intestinal microcirculatory beds via ETA receptors, which are closely related to increased production of PAF and decreased synthesis of constitutive nitric oxide.

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