Osteopontin inhibits inducible nitric oxide synthase activity in rat vascular tissue

1998 ◽  
Vol 275 (6) ◽  
pp. H2258-H2265 ◽  
Author(s):  
Jeremy A. Scott ◽  
M. Lynn Weir ◽  
Sylvia M. Wilson ◽  
Jim W. Xuan ◽  
Ann F. Chambers ◽  
...  

We tested the hypothesis that osteopontin (OPN) can inhibit the induction of inducible nitric oxide synthase (iNOS) in vascular tissue. iNOS activity was induced in rat thoracic aortas by incubation of the tissue with lipopolysaccharide (LPS) and measured by conversion ofl-[3H]arginine tol-[3H]citrulline. Addition of ≥1 nM recombinant OPN protein significantly reduced the LPS-induced increase in iNOS activity. Western blotting and the RT-PCR were used to determine the effect of LPS with and without OPN on tissue levels of iNOS protein and RNA, respectively. LPS resulted in an increase in iNOS protein and RNA, whereas OPN dose-dependently reduced tissue levels of iNOS activity, protein, and RNA. Mutated OPN proteins, in which the integrin-binding RGD amino acid sequence was deleted or mutated to RGE, resulted in complete and partial loss, respectively, of the ability of OPN to inhibit LPS-induced iNOS activity, implicating integrin binding in the effect. These results indicate that OPN can prevent induction of iNOS in vascular tissue.

Nitric Oxide ◽  
2001 ◽  
Vol 5 (2) ◽  
pp. 208-211 ◽  
Author(s):  
Jean-Charles Preiser ◽  
Haibo Zhang ◽  
Bernard Vray ◽  
Andreas Hrabak ◽  
Jean-Louis Vincent

2001 ◽  
Vol 132 (8) ◽  
pp. 1631-1638 ◽  
Author(s):  
Anthony J Kane ◽  
Jane E Barker ◽  
Geraldine M Mitchell ◽  
David R B Theile ◽  
Rosalind Romero ◽  
...  

2010 ◽  
Vol 67 (2) ◽  
pp. 195-204 ◽  
Author(s):  
Emina Sudar ◽  
Branislava Dobutovic ◽  
Sanja Soskic ◽  
Vesna Mandusic ◽  
Zorica Zakula ◽  
...  

1999 ◽  
Vol 60 (2) ◽  
pp. 297-304 ◽  
Author(s):  
Walter Tschugguel ◽  
Christian Schneeberger ◽  
Gertrud Unfried ◽  
Gerald Bräutigam ◽  
Felix Stonek ◽  
...  

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