inducible nitric oxide synthase
Recently Published Documents


TOTAL DOCUMENTS

4303
(FIVE YEARS 157)

H-INDEX

137
(FIVE YEARS 5)

Author(s):  
Muhammed Ahmed Mesaik ◽  
Almas Jabeen ◽  
Maria Saeed ◽  
Zaheer Ul-Haq ◽  
Izzaddinn Elawad Ahmed ◽  
...  

Background: Smokeless tobacco (SLT) is traditionally used in Middle East countries. The several toxic constituents with potential carcinogenicity make it a serious human health risk. Literature regarding their effects on cardiac and cancer disease is lacking in Saudi Arabia. Objective: This study was conducted to investigate the adverse effect of 11 different samples of widely used SLT varieties from the Tabuk region - Saudi Arabia, on nitric oxide (NO) level and their potential risk on cardiovascular health, etiology and/or progression of cancers. Methods: Samples were collected from Tabuk, KSA and analyzed by the GC-MS technique. Nitric oxide inhibition was performed using J774.2 macrophages by the Griess method. The retrieved crystallized structure of human inducible nitric oxide synthase (iNOS) from Brookhaven Protein Data Bank Repository PDB I.D: 3E7G with 2.20Å resolution was further prepared by structure using the MOE.2019 tool. The compounds abstracted from 11 different Shammah varieties were sketched by the MOE-Builder tool. Minimization for both receptor and compounds was performed via AMBER99 and MMFF99X force field implemented in MOE. Results: Nine samples (4 - 11) showed a potent suppressive effect on NO production with IC50 values ranging between (16.9-20.4 µg/mL), respectively. The samples (1 & 2) exhibited a moderate level of inhibition with IC50 ranging between 33.2 and 57.4 µg/mL, respectively. Interestingly, sample 4 consisting of compounds (13-15, 19-26, 28) that mostly belongs to the group fatty acid ester and phthalic acid ester showed the most potent suppressive effect. Molecular docking results revealed that the current local SLT constituents presented noticeable potency in different extract samples. Conclusion: Variable suppressive effects on NO were detected in the current SLT samples, where sample 4 was the most potent among all. The extract of the latter exhibited molecular interaction with the first shell amino acid residues of Inducible nitric oxide synthase (iNOS), which may anchor the plasticity and selectivity of the compounds present in it. The samples (4 -11) showed a potent inhibitory effect on the NO, where compound 26 (Phthalic acid ester) is common, and its adequate concentration may account for augmented biological activity. These results may effectively highlight their adverse effects on cardiovascular health and etiology and/or progression of cancer and may help in strengthening the social and governmental efforts in minimizing the use of these substances.


Author(s):  
Y.V. Sirman ◽  
I.V. Savytskyi ◽  
N.I. Preys

Diabetic retinopathy according to the World Health Organization reports is known as the main cause of decreased vision and blindness in diabetes. The purpose of the study is to analysis of changes in the activity of inducible nitric oxide synthase in experimental diabetic retinopathy and in various methods of its correction. Materials and methods. The research was carried out on white Wistar rats weighting 180-220 g. The animals were divided into 7 groups: 1st group included 60 intact animals; 2nd group involved 60 animals with modelled diabetic retinopathy (DR) without further correction; 3rd group included 60 animals with modelled DR and subsequent hyperglycemia correction; 4th group included 60 animals with modelled DR, which received subsequent hyperglycemia correction with aflibercept and L-arginine solution administration; 5th group consisted of 60 animals with modelled DR and subsequent hyperglycemia correction by aflibercept and bromfenac administration; 6th group was formed by 60 animals with modelled DR and subsequent hyperglycemia correction by aflibercept, L-carnitine and bromfenac administration; and the 7th group included 60 animals with modelled DR and subsequent hyperglycemia correction by aflibercept, L-arginine solution and citicoline. Results and conclusion. The results obtained demonstrate an increase in the activity of inducible nitric oxide synthase, starting from the 30th day of the experiment and with subsequent progression to the 60 and 108 days of experimental diabetic retinopathy that points out the deterioration of the physiological pathway of nitric oxide synthesis. Correction with hypoglycemic agents in group 3 had a positive effect, but was not able to reduce the activity of inducible nitric oxide synthase, which increased in the 2nd and 3rd stages, thus, there was a need for additional agents. The use of aflibercept and nitric oxide donor in group 4 to correct the progression of diabetic retinopathy significantly reduced the activity of inducible nitric oxide synthase (most effectively on the one hundred and eighty days of the experiment), but did not reach the control values. It has been proved that the correction applied in groups 5 and 6 significantly reduced the activity of inducible nitric oxide synthase but did not reach the control values. Moreover, the marker activity in the group 5 grew up on the 108 day. The study has shown the most effective correction includes a combination of metformin, aflibercept, L-arginine and citicoline given to the rats of group 7, as evidenced by the normalization of malonic dialdehyde levels on the 60 day of the experiment; on the 108 days of experiment there has been a decrease in marker content to control values.


Molecules ◽  
2021 ◽  
Vol 26 (22) ◽  
pp. 6861
Author(s):  
Thanh-Hao Huynh ◽  
Choo-Aun Neoh ◽  
Yu-Chi Tsai ◽  
Zhi-Kang Yao ◽  
Li-Guo Zheng ◽  
...  

A known polyoxygenated briarane, briaexcavatolide P (1), was isolated from a Formosan octocoral Briareum stechei. Moreover, the same species B. stechei, collected from Okinawan waters, yielded three chlorine-containing briaranes, including two new compounds, briastecholides B (2) and C (3) as well as a known analogue, briarenol R (4). The structures of 1–4 were established using spectroscopic methods. In addition, briarane 1 demonstrated anti-inflammatory activity in lipo-polysaccharide-induced RAW 264.7 mouse macrophage cells by suppressing the expression of inducible nitric oxide synthase (iNOS) protein.


2021 ◽  
Vol 16 (11) ◽  
pp. 1934578X2110592
Author(s):  
Yi-Ying Wu ◽  
Chia-Ching Hsieh ◽  
Chia-Ying Li ◽  
Wen-Huei Chang ◽  
Jih-Jung Chen ◽  
...  

Chemical examination of Sinularia querciformis afforded one new cembranoid, querciformolide F (1), along with four known cembranoids, sinulariolone (2), granosolide A (3), querciformolide A (4), and sinulariolide (5). The structures of these compounds were determined by extensive spectroscopic (IR, ESIMS, 1H NMR, and 13C NMR) analysis and by comparison with those previously reported in the literature. Compounds 2 to 4 were found to exhibit significant anti-inflammatory activity in lipopolysaccharide (LPS)-induced RAW264.7 mouse macrophages through attenuating the expression of the inducible nitric oxide synthase (iNOS) proteins.


Sign in / Sign up

Export Citation Format

Share Document