Succinic and Malic Oxidase in Gastric Hydrochloric Acid Production

1956 ◽  
Vol 187 (3) ◽  
pp. 427-431 ◽  
Author(s):  
Joseph J. Vitale ◽  
Oscar M. Jankelson ◽  
Patricia Connors ◽  
D. Mark Hegsted ◽  
Norman Zamcheck
Keyword(s):  
Gastric Mucosa ◽  
Guinea Pig ◽  
Succinic Dehydrogenase ◽  
Titratable Acidity ◽  
Spectrophotometric Analysis ◽  
Parietal Cells ◽  
Human Gastric Mucosa ◽  
City Hospital ◽  
Malic Dehydrogenase ◽  
Oxidase System

Effect of histamine on the activity of succinic oxidase and malic dehydrogenase was studied in guinea pig and human gastric mucosa. Human tissue was obtained through the surgical services of the Boston City Hospital. Control value for the succinic oxidase system of the proximal half of the guinea pig stomach was approximately 480 ( Qo2 (N) (µl O2/mg nitrogen/hr.)). After histamine, this value rose to 550 in 30 minutes with a simultaneous rise in titratable acidity of the stomach contents. Animals fasted for 72 hours had a Qo2 (N) of approximately 500 and after histamine a Qo2 (N) of 700 was observed. Spectrophotometric analysis of succinic dehydrogenase and cytochrome oxidase activities, two of the major components of the succinic oxidase system, revealed that both components are increased following histamine administration. Malic dehydrogenase, however, was not affected by histamine treatment. Succinic dehydrogenase was demonstrated by histochemical localization and was concentrated below the superficial mucous layer where parietal cells were abundant. Succinic oxidase activity of human gastric mucosa was demonstrable only in those specimens containing abundant parietal cells. This study confirms the view that HCl production by parietal cells is associated with aerobic metabolism and is perhaps under enzymatic control. The study suggests that the succinic oxidase system may be involved in the production or secretion of HCl.

Concomitant Staining of Mast and Parietal Cells in Human Gastric Mucosa

1996 ◽  
Vol 71 (5) ◽  
pp. 234-236 ◽  
Author(s):  
Dorota Biernacka ◽  
Alicja Tomaszewska ◽  
Jacek Muszynski ◽  
Stanislaw Moskalewski
Keyword(s):  
Gastric Mucosa ◽  
Parietal Cells ◽  
Human Gastric Mucosa

Lectin binding to parietal cells of human gastric mucosa.

1986 ◽  
Vol 34 (2) ◽  
pp. 237-243 ◽  
Author(s):  
N Kessimian ◽  
B J Langner ◽  
P N McMillan ◽  
H O Jauregui
Keyword(s):  
Gastric Mucosa ◽  
Binding Capacity ◽  
Lectin Binding ◽  
Binding Activity ◽  
Parietal Cells ◽  
Human Gastric Mucosa ◽  
Peanut Agglutinin ◽  
Intense Staining ◽  
Lectin Receptors ◽  
Ulex Europaeus

A light microscopic and ultrastructural analysis of lectin receptors on parietal cells from human gastric mucosa was performed utilizing 12 biotinylated lectins in conjunction with an avidin-biotin-peroxidase complex. Peanut agglutinin conjugated directly to peroxidase was also used. Several fixatives and fixation regimens were evaluated for optimal preservation of parietal cell saccharide moieties. Formalin proved to be the most practical fixative for light microscopic studies. A periodate-lysine-paraformaldehyde (PLP) combination provided good preservation of lectin binding capacity but yielded relatively poor ultrastructure. Conversely, glutaraldehyde provided excellent preservation of ultrastructure but a somewhat diminished lectin binding activity, which was overcome by using long incubation times and high concentrations of reagents. Parietal cells reacted strongly with Bandieraea simplicifolia, Dolichos biflorus, peanut agglutinin, and soybean agglutinin (all specific for galactosyl/galactosaminyl groups) and weakly with Ulex europaeus (specific for fucose). At the light microscopic level a beaded, perinuclear staining pattern was observed which, ultrastructurally, corresponded to an intense staining of intracytoplasmic canaliculi. The membranes of the intracytoplasmic canaliculi were characterized by an abundance of galactosyl residues, a paucity of fucosyl groups, and a lack of mannosyl and glucosyl residues. The biochemical and physiological significance of these findings is discussed.

scholarly journals Immunocytochemical identification of low-affinity NTS2 neurotensin receptors in parietal cells of human gastric mucosa

2006 ◽  
Vol 191 (1) ◽  
pp. 121-128 ◽  
Author(s):  
Stefan Schulz ◽  
Christoph Röcken ◽  
Matthias P A Ebert ◽  
Solveig Schulz
Keyword(s):  
Plasma Membrane ◽  
Gastric Mucosa ◽  
Acid Secretion ◽  
Gastric Acid Secretion ◽  
Gastric Acid ◽  
Parietal Cells ◽  
Neuroendocrine Cells ◽  
Target Cells ◽  
Human Gastric Mucosa ◽  
Western Blots

The biological effects of neurotensin (NT) are mediated by two distinct G protein-coupled receptors, NTS1 and NTS2. Although it is well established that neurotensin inhibits gastric acid secretion in man, the plasma membrane receptor mediating these effects has not been visualized yet. We developed and characterized a novel antipeptide antibody to the carboxy-terminal region of the human NTS2 receptor. The cellular and subcellular distribution of NTS2 receptors was evaluated in various human gastrointestinal tissues. Specificity of the antiserum was demonstrated by (1) detection of a broadband migrating at Mr 90 000–100 000 in Western blots of membranes from NTS2-expressing tissues; (2) cell-surface staining of NTS2-transfected cells; (3) translocation of NTS2 receptor immunostaining after agonist exposure; and (4) abolition of tissue immunostaining by preadsorbtion of the antibody with its immunizing peptide. In the gastrointestinal tract, NTS2 receptor immunoreactivity was highly abundant in parietal cells of the gastric mucosa, in neuroendocrine cells of the stomach small and large intestine, and in cells of the exocrine pancreas. NTS2 receptors were clearly located in the plasma membrane and uniformly present on nearly all target cells. The presence of NTS2 receptors was rarely detected in human tumors. This is the first localization of NTS2 receptors in human formalin-fixed, paraffin-embedded tissues at the cellular level. The abundant expression of low-affinity NTS2 receptors on the plasma membrane of human parietal cells provides a morphological substrate for the direct inhibition of gastric acid secretion observed after i.v. administration of neurotensin.

scholarly journals Isolation of parietal cells from guinea-pig gastric mucosa and the immunological characterization of their antigenic structure.

Gut ◽  
1975 ◽  
Vol 16 (8) ◽  
pp. 603-612 ◽  
Author(s):  
D P Jewell ◽  
V N Katiyar ◽  
C Rees ◽  
K B Taylor ◽  
J P Wright
Keyword(s):  
Gastric Mucosa ◽  
Guinea Pig ◽  
Parietal Cells ◽  
Antigenic Structure ◽  
Immunological Characterization

scholarly journals Cannabinoid CB1Receptors Are Expressed by Parietal Cells of the Human Gastric Mucosa

2008 ◽  
Vol 56 (5) ◽  
pp. 511-516 ◽  
Author(s):  
María Ruth Pazos ◽  
Rosa María Tolón ◽  
Cristina Benito ◽  
Conrado Fernández Rodríguez ◽  
Juan José Gorgojo ◽  
...  
Keyword(s):  
Gastric Mucosa ◽  
Parietal Cells ◽  
Human Gastric Mucosa

An Electron Microscopic Study of the Human Gastric Epithelia with Special Reference to the Ultrastructural Changes of the Parietal cells Induced by “Histalog“

1972 ◽  
Vol 30 ◽  
pp. 346-347
Author(s):  
Hiroshi Saito ◽  
Goro Asano ◽  
Kaoru Aihara ◽  
Katsunari Fukushi ◽  
Minoru Yoshida ◽  
...  
Keyword(s):  
Electron Microscope ◽  
Gastric Mucosa ◽  
Atrophic Gastritis ◽  
Endoscopic Biopsy ◽  
Parietal Cells ◽  
Ultrastructural Changes ◽  
Human Gastric Mucosa ◽  
Electron Microscopic ◽  
Biopsy Specimens ◽  
Control Study

This is to communicate the ultrastructural changes of the parietal cells of the human gastric mucosa in “Histalog” stimulation.Gastro-endoscopic biopsy specimens from 48 adult patients suffering from various gastritic conditions are studied. The patients are roughly divided into two groups. The first group consists of the control study. In essentially intact gastric mucosa (hyperacidic) cases, the “Histolog” 50mg had been injected intramuscularly to the subjects and chronologically endoscopic biopsy study had been achieved at 5, 10, 15, 20, 30, and 60 minutes respectively. The second group consists of the study with advanced atrophic gastritis (anacidity) and similar manipulation has been attemped. The obtained specimens were quickly fixed in 2.5% glutaraldehyde, postosmificated, and dehydrated in graded alcohols and embedded in Epon 812. Sections were cut and observed with the Hitachi 11U-B electron microscope with 50kv or 75kv accelerating voltages.

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