scholarly journals Hepatitis B Surface Antigen Could Contribute to the Immunopathogenesis of Hepatitis B Virus Infection

2013 ◽  
Vol 2013 ◽  
pp. 1-8 ◽  
Author(s):  
Yasuteru Kondo ◽  
Masashi Ninomiya ◽  
Eiji Kakazu ◽  
Osamu Kimura ◽  
Tooru Shimosegawa
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Natural Killer ◽  
Surface Antigen ◽  
Persistent Infection ◽  
Hepatitis B Surface Antigen ◽  
Immune Therapy ◽  
Chronic Phase ◽  
Hbv Replication ◽  
B Virus

Various findings concerning the clinical significance of quantitative changes in hepatitis B surface antigen (HBsAg) during the acute and chronic phase of hepatitis B virus (HBV) infection have been reported. In addition to being a biomarker of HBV-replication activity, it has been reported that HBsAg could contribute to the immunopathogenesis of HBV persistent infection. Moreover, HBsAg could become an attractive target for immune therapy, since the cellular and humeral immune response against HBsAg might be able to control the HBV replication and life cycle. However, several reports have described the immune suppressive function of HBsAg. HBsAg might suppress monocytes, dendritic cells (DCs), natural killer (NK), and natural killer T (NK-T) cells by direct interaction. On the other hand, cytotoxic T lymphocytes (CTLs) and helper T (Th) cells were exhausted by high amounts of HBsAg. In this paper, we focused on the immunological aspects of HBsAg, since better understanding of the interaction between HBsAg and immune cells could contribute to the development of an immune therapy as well as a biomarker of the state of HBV persistent infection.

Interferon-Induced Macrophage-Derived Exosomes Mediate Antiviral Activity Against Hepatitis B Virus Through miR-574-5p

2020 ◽  
Author(s):  
Wenyu Wu ◽  
Di Wu ◽  
Weiming Yan ◽  
Yongli Wang ◽  
Jie You ◽  
...  
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Surface Antigen ◽  
Genomic Sequence ◽  
Hepatitis B Surface Antigen ◽  
Circular Dna ◽  
Hbv Replication ◽  
Covalently Closed Circular Dna ◽  
B Virus ◽  
Antiviral Activities

Abstract Background Interferon alfa (IFN-α) has been proved effective in treating chronic hepatitis B (CHB), owing to its ability to suppress hepatitis B surface antigen and hepatitis B virus (HBV) covalently closed circular DNA. However, the underlying mechanisms are unclear. Methods We investigated the antiviral activities of exosomes from responders and nonresponders to pegylated IFN-α (PegIFN-α) as well as the supernatants of IFN-α–treated macrophages derived from THP-1 (the human leukemia monocyte cell line). Then the expression profiles of exosomal microRNAs (miRNAs) were analyzed using miRNA sequencing. The luciferase reporter assay was used to locate the binding position of HBV genomic sequence targeted by the identified miRNA. Results Exosomes from PegIFN-α–treated patients, particularly responders, as well as the supernatants of IFN-α–treated macrophages exhibited anti-HBV activities, as manifested by the suppression of hepatitis B surface antigen, hepatitis B e antigen, HBV DNA, and covalently closed circular DNA levels in HBV-related cell lines. PegIFN-α treatment up-regulated exosomal hsa-miR-193a-5p, hsa-miR-25-5p, and hsa-miR-574-5p, which could partially inhibit HBV replication and transcription, and hsa-miR-574-5p reduced pregenomic RNA and polymerase messenger RNA levels by binding to the 2750–2757 position of the HBV genomic sequence. Conclusions Exosomes can transfer IFN-α–related miRNAs from macrophages to HBV-infected hepatocytes, and they exhibit antiviral activities against HBV replication and expression.

Discrepant Hepatitis B Surface Antigen Results in Pregnant Women Screened to Identify Hepatitis B Virus Infection

2014 ◽  
Vol 165 (4) ◽  
pp. 773-778 ◽  
Author(s):  
Steven L. Veselsky ◽  
Tanja Y. Walker ◽  
Nancy Fenlon ◽  
Chong-Gee Teo ◽  
Trudy V. Murphy
Keyword(s):  
Hepatitis B Virus ◽  
Virus Infection ◽  
Hepatitis B ◽  
Pregnant Women ◽  
Surface Antigen ◽  
Hepatitis B Surface Antigen ◽  
Hepatitis B Virus Infection ◽  
B Virus

Sensitivity of hepatitis B virus DNA transcription-mediated amplification testing in hepatitis B surface antigen?positive blood donations

Transfusion ◽  
2006 ◽  
Vol 46 (12) ◽  
pp. 2047-2052 ◽  
Author(s):  
Françoise Bouchardeau ◽  
Annie Girault ◽  
Annie Razer ◽  
Annabelle Servant-Delmas ◽  
Mélanie Mercier ◽  
...  
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Surface Antigen ◽  
Hepatitis B Surface Antigen ◽  
Blood Donations ◽  
Dna Transcription ◽  
Hepatitis B Virus Dna ◽  
B Virus

Enhanced production of hepatitis B surface antigen in NIH 3T3 mouse fibroblasts by using extrachromosomally replicating bovine papillomavirus vector

1983 ◽  
Vol 3 (6) ◽  
pp. 1032-1039
Author(s):  
Y Wang ◽  
C Stratowa ◽  
M Schaefer-Ridder ◽  
J Doehmer ◽  
P H Hofschneider
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Surface Antigen ◽  
Hepatitis B Surface Antigen ◽  
Bovine Papillomavirus ◽  
Mouse Fibroblasts ◽  
Antigen Gene ◽  
B Virus ◽  
Nih 3T3 ◽  
Papillomavirus Dna

We have constructed a recombinant pBR322 plasmid composed of a subgenomic transforming fragment of bovine papillomavirus DNA and the hepatitis B surface antigen gene from cloned hepatitis B virus DNA and used it for transfection of NIH 3T3 mouse fibroblasts. The transformed cells retain the plasmids in extrachromosomal form with a copy number of about 50 to 100 per cell. Expression of the hepatitis B surface antigen gene linked to bovine papillomavirus DNA is independent of its orientation relative to the bovine papillomavirus vector. Cell lines continuously secreting high amounts of hepatitis B surface antigen into the medium could be established. The antigen is released into the culture medium as 22-nm particles, having the same physical properties and constituent polypeptides as those found in the serum of hepatitis B virus-infected patients.

Perinatal Transmitted Acute Icteric Hepatitis B in Infants Born to Hepatitis B Surface Antigen-Positive and Anti-hepatitis Be-Positive Carrier Mothers

PEDIATRICS ◽  
1982 ◽  
Vol 70 (4) ◽  
pp. 557-559
Author(s):  
Frank R. Sinatra ◽  
Praful Shah ◽  
Joy Y. Weissman ◽  
Daniel W. Thomas ◽  
Russell J. Merritt ◽  
...  
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Surface Antigen ◽  
Hepatitis B Surface Antigen ◽  
B Virus ◽  
Positive Hbeag ◽  
Hbeag Negative Carrier ◽  
Positive Carrier

Three infants born to mothers who were hepatitis B surface antigen (HBsAg) positive and had antibody to hepatitis Be antigen (anti-HBe), developed acute icteric hepatitis B within three months of birth. All three infants clinically recovered and developed circulating anti-HBs. Contrary to previous studies, these three cases indicate that mother-infant transmission of the hepatitis B virus (HBV) does occur in infants born to HBsAg-positive, HBeAg-negative carrier mothers, and these infants may develop severe acute icteric hepatitis. Therefore, immunoprophylaxis in such newborns may be indicated.

Sign in / Sign up

Export Citation Format

Share Document