scholarly journals Pharmacokinetic and Biodistribution Assessment of a Near Infrared-Labeled PSMA-Specific Small Molecule in Tumor-Bearing Mice

2014 ◽  
Vol 2014 ◽  
pp. 1-10 ◽  
Author(s):  
Joy L. Kovar ◽  
Lael L. Cheung ◽  
Melanie A. Simpson ◽  
D. Michael Olive

Prostate cancer is the most frequently diagnosed cancer in men and often requires surgery. Use of near infrared (NIR) technologies to perform image-guided surgery may improve accurate delineation of tumor margins. To facilitate preclinical testing of such outcomes, here we developed and characterized a PSMA-targeted small molecule, YC-27. IRDye 800CW was conjugated to YC-27 or an anti-PSMA antibody used for reference. Human 22Rv1, PC3M-LN4, and/or LNCaP prostate tumor cells were exposed to the labeled compounds.In vivotargeting and clearance properties were determined in tumor-bearing mice. Organs and tumors were excised and imaged to assess probe localization. YC-27 exhibited a dose dependent increase in signal upon binding. Binding specificity and internalization were visualized by microscopy.In vitroandin vivoblocking studies confirmed YC-27 specificity.In vivo, YC-27 showed good tumor delineation and tissue contrast at doses as low as 0.25 nmole. YC-27 was cleared via the kidneys but bound the proximal tubules of the renal cortex and epididymis. Since PSMA is also broadly expressed on the neovasculature of most tumors, we expect YC-27 will have clinical utility for image-guided surgery and tumor resections.

2018 ◽  
Vol 9 (8) ◽  
pp. 2092-2097 ◽  
Author(s):  
Yao Sun ◽  
Xiaodong Zeng ◽  
Yuling Xiao ◽  
Changhao Liu ◽  
Hua Zhu ◽  
...  

The first small-molecule based αvβ3-targeted NIR-II/PET dual-modal probesviabase-catalyzed thiol-addition chemistry were concisely assembled and evaluated.


2017 ◽  
Vol 8 (5) ◽  
pp. 3489-3493 ◽  
Author(s):  
Yao Sun ◽  
Mingmin Ding ◽  
Xiaodong Zeng ◽  
Yuling Xiao ◽  
Huaping Wu ◽  
...  

This work presents the establishment of novel bright-emission small-molecule NIR-II fluorophores forin vivotumor imaging and NIR-II image-guided sentinel lymph node surgery.


2014 ◽  
Author(s):  
Paul Dorval ◽  
Norman Mangeret ◽  
Stephanie Guillermet ◽  
Christian Adrien Righini ◽  
Gabriele Barabino ◽  
...  

2010 ◽  
Vol 97 (5) ◽  
pp. 737-743 ◽  
Author(s):  
M. Keramidas ◽  
V. Josserand ◽  
C. A. Righini ◽  
C. Wenk ◽  
C. Faure ◽  
...  

2016 ◽  
Vol 59 (11) ◽  
pp. 5311-5323 ◽  
Author(s):  
Eric A. Owens ◽  
Hoon Hyun ◽  
Tyler L. Dost ◽  
Jeong Heon Lee ◽  
GwangLi Park ◽  
...  

2019 ◽  
Vol 31 (2) ◽  
pp. 375-395 ◽  
Author(s):  
Albertus Wijnand Hensbergen ◽  
Danny M. van Willigen ◽  
Florian van Beurden ◽  
Pim J. van Leeuwen ◽  
Tessa Buckle ◽  
...  

Small ◽  
2019 ◽  
Vol 15 (45) ◽  
pp. 1903382 ◽  
Author(s):  
Qing Zhang ◽  
Huijun Zhou ◽  
Hao Chen ◽  
Xiao Zhang ◽  
Shuqing He ◽  
...  

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