scholarly journals Production and Structural Characterization ofLactobacillus helveticusDerived Biosurfactant

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
Deepansh Sharma ◽  
Baljeet Singh Saharan ◽  
Nikhil Chauhan ◽  
Anshul Bansal ◽  
Suresh Procha

A probiotic strain oflactobacilliwas isolated from traditional soft Churpi cheese of Yak milk and found positive for biosurfactant production.Lactobacillireduced the surface tension of phosphate buffer saline (PBS) from 72.0 to 39.5 mNm−1pH 7.2 and its critical micelle concentration (CMC) was found to be 2.5 mg mL−1. Low cost production ofLactobacilliderived biosurfactant was carried out at lab scale fermenter which yields 0.8 mg mL−1biosurfactant. The biosurfactant was found least phytotoxic and cytotoxic as compared to the rhamnolipid and sodium dodecyl sulphate (SDS) at different concentration. Structural attributes of biosurfactant were determined by FTIR, NMR (1H and13C), UPLC-MS, and fatty acid analysis by GCMS which confirmed the presence of glycolipid type of biosurfactant closely similar to xylolipids. Biosurfactant is mainly constituted by lipid and sugar fractions. The present study outcomes provide valuable information on structural characterization of the biosurfactant produced byL. helveticusMRTL91. These findings are encouraging for the application ofLactobacilliderived biosurfactant as nontoxic surface active agents in the emerging field of biomedical applications.

2020 ◽  
pp. 9-14 ◽  
Author(s):  
Acharya Anil Ramchandra ◽  
R. Kadam ◽  
A. T. Pise

Here the investigations are done while distillation of ethanol-water mixture for separating ethanol from fermentation process. Focus is to study reduction in time required and hence saving in energy for the distillation process of ethanol-water mixture under the influence of surface-active agents (Surfactants). This novelty is from observation of these surfactants to enhance heat transfer rate because of surface tension reduction in aqueous solutions. SDS (Sodium Dodecyl Sulphate), NH4Cl (Ammonium Chloride) and SLBS (Sodium lauryl benzene sulphonate) surfactants in different concentration are experimented. The concentration of these surfactant is varied from 1700 ppm to 2800 ppm. This range is decided by observing critical micelle concentration of used surfactants. Results showed that time is reduced and hence energy consumption is also reduced. Results shown by NH4Cl are found to be more useful as it is ecofriendly surfactant which is not affecting ethanol-water mixture. Use of ammonium chloride as surfactant in distillation is actually useful to reduce energy without hampering the quality of process is the novelty of this work.


1992 ◽  
Vol 26 (1-2) ◽  
pp. 377-386 ◽  
Author(s):  
F. Çeçen

Laboratory-scale physicochemical and biological treatability studies were performed on wastewaters discharged from an industry producing household and heavy-duty detergents of powder and liquid type. The characterization of effluents led to the conclusion that the industry was highly pollutant in terms of BOD5, COD, surfactant, phosphorus, oil-grease, suspended solids and pH. By the use of lime in the precipitation stage about 80 % of COD and more than 90 % of phosphorus and anionic surface active agents could be removed. Following the treatment with lime the biodegradation of these wastewaters was investigated in continuously fed activated sludge units. The biokinetic constants of the reaction were determined as k = 0.76 d−1, Ks = 972 mg/l COD, a = 0.58 mg O2/mg COD, b = 0.044 mg O2/mg MLVSS.d. The experimental findings were used in the design of a full-scale treatment system.


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