scholarly journals Training Effects on ROS Production Determined by Electron Paramagnetic Resonance in Master Swimmers

2015 ◽  
Vol 2015 ◽  
pp. 1-8 ◽  
Author(s):  
Simona Mrakic-Sposta ◽  
Maristella Gussoni ◽  
Simone Porcelli ◽  
Lorenzo Pugliese ◽  
Gaspare Pavei ◽  
...  
Keyword(s):  
Electron Paramagnetic Resonance ◽  
Oxygen Consumption ◽  
Antioxidant Capacity ◽  
Acute Exercise ◽  
Peak Oxygen Consumption ◽  
Strenuous Exercise ◽  
Ros Production ◽  
Paramagnetic Resonance ◽  
Ergometer Exercise ◽  
Electron Paramagnetic

Acute exercise induces an increase in Reactive Oxygen Species (ROS) production dependent on exercise intensity with highest ROS amount generated by strenuous exercise. However, chronic repetition of exercise, that is, exercise training, may reduce exercise-induced oxidative stress. Aim of this study was to evaluate the effects of 6-weeks high-intensity discontinuous training (HIDT), characterized by repeated variations of intensity and changes of redox potential, on ROS production and antioxidant capacity in sixteen master swimmers. Time course changes of ROS generation were assessed by Electron Paramagnetic Resonance in capillary blood by a microinvasive approach. An incremental arm-ergometer exercise (IE) until exhaustion was carried out at both before (PRE) and after (POST) training (Trg) period. A significant (P<0.01) increase of ROS production from REST to the END of IE in PRE Trg (2.82±0.66versus3.28±0.66 µmol·min−1) was observed. HIDT increased peak oxygen consumption (36.1±4.3versus40.6±5.7 mL·kg−1·min−1PRE and POST Trg, resp.) and the antioxidant capacity (+13%) while it significantly decreased the ROS production both at REST (−20%) and after IE (−25%). The observed link between ROS production, adaptive antioxidant defense mechanisms, and peak oxygen consumption provides new insight into the correlation between ROS response pathways and muscle metabolic function.

scholarly journals Determination of erythrocyte antioxidant capacity in haemodialysis patients using electron paramagnetic resonance

2001 ◽  
Vol 16 (11) ◽  
pp. 2166-2171 ◽  
Author(s):  
Andree Klemm ◽  
Christine Voigt ◽  
Manfred Friedrich ◽  
Reinhard Fünfstück ◽  
Heide Sperschneider ◽  
...  
Keyword(s):  
Electron Paramagnetic Resonance ◽  
Antioxidant Capacity ◽  
Paramagnetic Resonance ◽  
Electron Paramagnetic

Oxygen consumption by peritoneal macrophages measured by electron paramagnetic resonance

1985 ◽  
Vol 99 (4) ◽  
pp. 464-466
Author(s):  
A. V. Yakh'ev ◽  
A. N. Osipov ◽  
O. A. Azizova ◽  
L. G. Korkina ◽  
B. T. Velichkovskii ◽  
...  
Keyword(s):  
Electron Paramagnetic Resonance ◽  
Oxygen Consumption ◽  
Peritoneal Macrophages ◽  
Paramagnetic Resonance ◽  
Electron Paramagnetic

scholarly journals A Quantitative Method to Monitor Reactive Oxygen Species Production by Electron Paramagnetic Resonance in Physiological and Pathological Conditions

2014 ◽  
Vol 2014 ◽  
pp. 1-10 ◽  
Author(s):  
Simona Mrakic-Sposta ◽  
Maristella Gussoni ◽  
Michela Montorsi ◽  
Simone Porcelli ◽  
Alessandra Vezzoli
Keyword(s):  
Oxidative Stress ◽  
Reactive Oxygen Species ◽  
Electron Paramagnetic Resonance ◽  
Venous Blood ◽  
Reactive Oxygen ◽  
Capillary Blood ◽  
Ros Production ◽  
Oxygen Species ◽  
Paramagnetic Resonance ◽  
Electron Paramagnetic

The growing interest in the role of Reactive Oxygen Species (ROS) and in the assessment of oxidative stress in health and disease clashes with the lack of consensus on reliable quantitative noninvasive methods applicable. The study aimed at demonstrating that a recently developed Electron Paramagnetic Resonance microinvasive method provides direct evidence of the “instantaneous” presence of ROS returning absolute concentration levels that correlate with “a posteriori” assays of ROS-induced damage by means of biomarkers. The reliability of the choice to measure ROS production rate in human capillary blood rather than in plasma was tested (step I). A significant (P<0.01) linear relationship between EPR data collected on capillary blood versus venous blood (R2=0.95), plasma (R2=0.82), and erythrocytes (R2=0.73) was found. Then (step II) ROS production changes of various subjects’ categories, young versus old and healthy versus pathological at rest condition, were found significantly different (range 0.0001–0.05Plevel). The comparison of the results with antioxidant capacity and oxidative damage biomarkers concentrations showed that all changes indicating increased oxidative stress are directly related to ROS production increase. Therefore, the adopted method may be an automated technique for a lot of routine in clinical trials.

scholarly journals R(+)-Thioctic Acid Effects on Oxidative Stress and Peripheral Neuropathy in Type II Diabetic Patients: Preliminary Results by Electron Paramagnetic Resonance and Electroneurography

2018 ◽  
Vol 2018 ◽  
pp. 1-15 ◽  
Author(s):  
Simona Mrakic-Sposta ◽  
Alessandra Vezzoli ◽  
Luca Maderna ◽  
Francesca Gregorini ◽  
Michela Montorsi ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Electron Paramagnetic Resonance ◽  
Production Rate ◽  
Conduction Velocity ◽  
Nerve Conduction ◽  
Nerve Conduction Velocity ◽  
Ros Production ◽  
Thioctic Acid ◽  
Paramagnetic Resonance ◽  
Electron Paramagnetic

Objectives. Diabetic neuropathy is the most common complication of diabetes. The idea of alterations in energy metabolism in diabetes is emerging. The biogenic antioxidant R(+)-thioctic acid has been successfully used in the treatment of diabetic polyneuropathic (DPN) patients. Methods. The effects of R(+)-thioctic acid (1 tablet, 1.6 g) administration were evaluated in 12 DPN patients at baseline and at 15, 30, 60, and 120 administration days throughout the assessment of oxidative stress (OxS); ROS production rate by electron paramagnetic resonance (EPR) technique; and oxidative damage biomarkers (thiobarbituric acid reactive substances (TBARS) and protein carbonyls (PC)), electroneurography (ENG) and visual analogue scale. Results. Supplementation induced significant changes (p<0.05) at 30 and 60 days. ROS production rate up to −16%; TBARS (−31%), PC (−38%), and TAC up to +48%. Motor nerve conduction velocity in SPE and ulnar nerves (+22% and +16%) and sensor conduction velocity in sural and median nerves (+22% and +5%). Patients reported a general wellness sensation improvement (+35%) at 30 days: lower limb pain sensation (−40%) and upper limbs (−23%). Conclusion. The results strongly indicate that an increased antioxidant capacity plays an important role in OxS, nerve conduction velocity, pain, and general wellness improvement. Nevertheless, the effects of the antioxidant compound were found positive up to 60 days. Then, a hormesis effect was observed. Novelty of the research would be a challenge for investigators to carefully address issues, including dose range factors, appropriate administration time, and targeting population to counteract possible “boomerang effects.” The great number of monitored parameters would firmly stress these conclusions.

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