scholarly journals Mouse IgG2a Antibodies Specific for the Commensal Streptococcus mitis Show Stronger Cross-Reactivity with Streptococcus pneumoniae than IgG1 Antibodies

2019 ◽  
Vol 2019 ◽  
pp. 1-4
Author(s):  
Sudhanshu Shekhar ◽  
Rabia Khan ◽  
Ata Ul Razzaq Khan ◽  
Fernanda Cristina Petersen
Keyword(s):  
Streptococcus Pneumoniae ◽  
Pneumococcal Vaccine ◽  
Cross Reactivity ◽  
Streptococcus Mitis ◽  
The Cross ◽  
Mouse Igg2a

Here we show that mouse IgG2a and IgG1 antibodies specific for the commensal Streptococcus mitis cross-react with pathogen Streptococcus pneumoniae serotypes 2 and 4, although the cross-reactivity conferred by IgG2a is stronger than that by IgG1 antibodies. These findings may be important for understanding the S. mitis-induced IgG isotype responses and have consequences for the development of an effective pneumococcal vaccine.

scholarly journals Mouse IgG2a antibodies specific for the commensal Streptococcus mitis show stronger cross-reactivity with Streptococcus pneumoniae than IgG1 antibodies

2019 ◽  
Author(s):  
Sudhanshu Shekhar ◽  
Rabia Khan ◽  
Ata Ul Razzaq Khan ◽  
Fernanda Cristina Petersen
Keyword(s):  
Streptococcus Pneumoniae ◽  
Cross Reactivity ◽  
Pneumococcal Infections ◽  
Streptococcus Mitis ◽  
The Cross ◽  
Mouse Igg2a

AbstractHere we show that mouse IgG2a and IgG1 antibodies specific for the commensal Streptococcus mitis cross-react with the pathogen Streptococcus pneumoniae, although the cross-reactivity conferred by IgG2a is stronger than IgG1 antibodies. These findings may have implications for designing S. mitis-based vaccines against pneumococcal infections.

scholarly journals A Common Food Glycan, Pectin, Shares an Antigen with Streptococcus pneumoniae Capsule

mSphere ◽  
2020 ◽  
Vol 5 (2) ◽  
Author(s):  
Moon H. Nahm ◽  
Jigui Yu ◽  
Jiri Vlach ◽  
Maor Bar-Peled
Keyword(s):  
Immune Response ◽  
Streptococcus Pneumoniae ◽  
Immune Responses ◽  
Pneumococcal Vaccine ◽  
Cross Reactivity ◽  
Food Plants ◽  
Content Type ◽  
Plant Foods ◽  
Vaccine Responses ◽  
The Impact

ABSTRACT We are exposed daily to many glycans from bacteria and food plants. Bacterial glycans are generally antigenic and elicit antibody responses. It is unclear if food glycans’ sharing of antigens with bacterial glycans influences our immune responses to bacteria. We studied 14 different plant foods for cross-reactivity with monoclonal antibodies (MAbs) against 24 pneumococcal serotypes which commonly cause infections and are included in pneumococcal vaccines. Serotype 15B-specific MAb cross-reacts with fruit peels, and serotype 10A MAb cross-reacts with many natural and processed plant foods. The serotype 10A cross-reactive epitope is 1,6-β-galactosidase [βGal(1-6)], present in the rhamno-galacturonan I (RG-I) domain of pectin. Despite wide consumption of pectin, the immune response to 10A is comparable to the responses to other serotypes. An antipectin antibody can opsonize serotype 10A pneumococci, and the shared βGal(1-6) may be useful as a simple vaccine against 10A. Impact of food glycans should be considered in host-pathogen interactions and future vaccine designs. IMPORTANCE The impact of food consumption on vaccine responses is unknown. Streptococcus pneumoniae (the pneumococcus) is an important human pathogen, and its polysaccharide capsule is used as a vaccine. We show that capsule type 10A in a pneumococcal vaccine shares an antigenic epitope, βGal(1-6), with pectin, which is in many plant foods and is widely consumed. Immune response to 10A is comparable to that seen with other capsule types, and pectin ingestion may have little impact on vaccine responses. However, antibody to pectin can kill serotype 10A pneumococci and this shared epitope may be considered in pneumococcal vaccine designs.

scholarly journals Antibodies Reactive to Commensal Streptococcus mitis Show Cross-Reactivity With Virulent Streptococcus pneumoniae Serotypes

2018 ◽  
Vol 9 ◽  
Author(s):  
Sudhanshu Shekhar ◽  
Rabia Khan ◽  
Daniela M. Ferreira ◽  
Elena Mitsi ◽  
Esther German ◽  
...  
Keyword(s):  
Streptococcus Pneumoniae ◽  
Cross Reactivity ◽  
Streptococcus Mitis

Structural elucidation of the capsular polysaccharide of Streptococcus pneumoniae type 9N

1983 ◽  
Vol 61 (10) ◽  
pp. 1102-1107 ◽  
Author(s):  
Karl-Gunnar Rosell ◽  
Harold J. Jennings
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Streptococcus Pneumoniae ◽  
Magnetic Resonance ◽  
Capsular Polysaccharide ◽  
Glucuronic Acid ◽  
Cross Reactivity ◽  
Structural Elucidation ◽  
Molar Ratio ◽  
The Cross ◽  
Nuclear Magnetic

The specific capsular polysaccharide of Streptococcus pneumoniae type 9N (American type 9) contains D-glucose, D-glucuronic acid, 2-acetamido-2-deoxy-D-glucose, and 2-acetamido-2-deoxy-D-mannose in the molar ratio of 2:1:1:1. Accumulated data from spectroscopic (13C and 1H nuclear magnetic resonance) and methylation analyses of the native and specifically degraded polysaccharide indicated that it was linear and composed of the following pentasaccharide repeating unit; —4)-α-D-GlcpA-(1 → 3)-α-D-Glcp-(1 → 3)-β-D-ManpNAc-(1 → 4)-α-D-Glcp-(1 → 4)-β-D-GlcpNAc(1 →. Structural regions in the type 9N polysaccharide common to those of types 9A, 9L, and 9V have been identified which account for the cross-reactivity of this groups of polysaccharides.

scholarly journals Serotyping of Streptococcus pneumoniaeby Coagglutination with 12 Pooled Antisera

1999 ◽  
Vol 37 (1) ◽  
pp. 263-265 ◽  
Author(s):  
M. K. Lalitha ◽  
Kurien Thomas ◽  
R. Satish Kumar ◽  
Mark C. Steinhoff
Keyword(s):  
Streptococcus Pneumoniae ◽  
Pneumococcal Vaccine ◽  
Cross Reactivity ◽  
Typing Methods

We report on the performance of a recently introduced commercial chessboard method using 12 antisera, in comparison with that of the 55-antiserum panel used in determining the serogroups and types (SGTs) of Streptococcus pneumoniae, both of which were carried out by a coagglutination technique. Of a total of 150 strains of S. pneumoniae studied, 135 (90%) belonged to the SGTs represented in the 23-valent pneumococcal vaccine; of these, 130 (96.3%) were identified as the same SGTs by both typing methods. The remaining five strains showed cross-reactivity with more than two pools by the chessboard method, but could be assigned to a single SGT by the Quellung test. The 96.3% concordance of the chessboard method suggests it can be adopted for determination of the SGTs of S. pneumoniae in laboratories.

scholarly journals Analysis of Serum Cross-Reactivity and Cross-Protection Elicited by Immunization with DNA Vaccines against Streptococcus pneumoniae Expressing PspA Fragments from Different Clades

2002 ◽  
Vol 70 (9) ◽  
pp. 5086-5090 ◽  
Author(s):  
Eliane N. Miyaji ◽  
Daniela M. Ferreira ◽  
Alexandre P. Y. Lopes ◽  
M. Cristina C. Brandileone ◽  
Waldely O. Dias ◽  
...  
Keyword(s):  
Streptococcus Pneumoniae ◽  
Dna Vaccines ◽  
Protein A ◽  
Sequence Divergence ◽  
Surface Protein ◽  
Cost Effective ◽  
Cross Reactivity ◽  
Cross Protection ◽  
The Cross ◽  
Humoral Responses

ABSTRACT Streptococcus pneumoniae is a major cause of disease, especially in developing countries, and cost-effective alternatives to the currently licensed vaccines are needed. We constructed DNA vaccines based on pneumococcal surface protein A (PspA), an antigen shown to induce protection against pneumococcal bacteremia. PspA fragments can be divided into three families, which can be subdivided into six clades, on the basis of PspA amino acid sequence divergence (S. K. Hollingshead, R. Becker, and D. E. Briles, Infect. Immun. 68:5889-5900, 2000). Since most clinical isolates belong to family 1 or family 2, PspA fragments from members of both of these families were analyzed. Vectors encoding the complete N-terminal regions of PspAs elicited significant humoral responses, and cross-reactivity was mainly restricted to the same family. DNA vaccines encoding fusions between PspA fragments from family 1 and family 2 were also constructed and were able to broaden the cross-reactivity, with induction of antibodies that showed reactions with members of both families. At least for the pneumococcal strains tested, the cross-reactivity of antibodies was not reflected in cross-protection. Animals immunized with DNA vaccines expressing the complete N-terminal regions of PspA fragments were protected only against intraperitoneal challenge with a strain expressing PspA from the same clade.

scholarly journals Use and efficacy of pneumococcal vaccine in patients with Hodgkin disease

Blood ◽  
1979 ◽  
Vol 54 (5) ◽  
pp. 1171-1175 ◽  
Author(s):  
AM Levine ◽  
GD Overturf ◽  
RF Field ◽  
D Holdorf ◽  
A Paganini-Hill ◽  
...  
Keyword(s):  
Streptococcus Pneumoniae ◽  
Antibody Response ◽  
Haemophilus Influenzae ◽  
Pneumococcal Vaccine ◽  
Hodgkin Disease ◽  
Bacterial Sepsis ◽  
Severe Impairment ◽  
Postsplenectomy Sepsis ◽  
Previously Treated ◽  
Normal Controls

Abstract Fulminant bacterial sepsis has been described in patients with Hodgkin disease who have undergone splenectomy for staging purposes. The organisms commonly associated with sepsis in this setting include Streptococcus pneumoniae and Haemophilus influenzae. Polyvalent pneumococcal vaccine (Merck) has recently been licensed and has been suggested for use in patients with Hodgkin disease who are at risk for postsplenectomy sepsis. We administered 14-valent pneumococcal vaccine to 24 patients with Hodgkin disease and 24 normal controls, and measured antibody response to 13 antigens at time of immunization and at 3 wk and 3 mo following immunization. Our results indicate that patients who have been previously treated for Hodgkin disease, with chemotherapy, radiotherapy, or both, have severe impairment of antibody response. Untreated patients, however, respond in a manner similar to normal controls.

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