Suberoylanilide Hydroxamic Acid, a Histone Deacetylase Inhibitor: Effects on Gene Expression and Growth of Glioma Cells In vitro and In vivo

2007 ◽  
Vol 13 (3) ◽  
pp. 1045-1052 ◽  
Author(s):  
Dong Yin ◽  
John M. Ong ◽  
Jinwei Hu ◽  
Julian C. Desmond ◽  
Norihiko Kawamata ◽  
...  
Keyword(s):  
Gene Expression ◽  
Histone Deacetylase ◽  
Histone Deacetylase Inhibitor ◽  
Hydroxamic Acid ◽  
Glioma Cells ◽  
Suberoylanilide Hydroxamic Acid ◽  
Deacetylase Inhibitor

In vitro and in vivo histone deacetylase inhibitor therapy with suberoylanilide hydroxamic acid (SAHA) and paclitaxel in ovarian cancer

2007 ◽  
Vol 104 (3) ◽  
pp. 596-601 ◽  
Author(s):  
Amy L. Cooper ◽  
Victoria L. Greenberg ◽  
Pamela S. Lancaster ◽  
John R. van Nagell ◽  
Stephen G. Zimmer ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Histone Deacetylase ◽  
Histone Deacetylase Inhibitor ◽  
Hydroxamic Acid ◽  
Inhibitor Therapy ◽  
Suberoylanilide Hydroxamic Acid ◽  
Deacetylase Inhibitor

57 GENE EXPRESSION PATTERN OF MINIATURE PIG SOMATIC CELL NUCLEAR TRANSFER EMBRYOS TREATED WITH THE HISTONE DEACETYLASE INHIBITOR SCRIPTAID

2011 ◽  
Vol 23 (1) ◽  
pp. 134
Author(s):  
C. H. Park ◽  
S. G. Lee ◽  
H. J. Lee ◽  
T. K. Jung ◽  
Y. H. Jeong ◽  
...  
Keyword(s):  
Gene Expression ◽  
Histone Deacetylase ◽  
Expression Pattern ◽  
Histone Deacetylase Inhibitor ◽  
Gene Expression Pattern ◽  
Control Group ◽  
Developmental Potential ◽  
Deacetylase Inhibitor

It was recently shown that treatment of cloned embryos with histone deacetylase inhibitors improves efficiency for the success rate of developmental potential to term in several species. The objective of the present study was to investigate the influence of the histone deacetylase inhibitor Scriptaid (Sc) on in vitro development in early porcine SCNT embryos and on their gene expression pattern. Based on the findings of previous porcine studies (Zhao et al. 2009), the reconstructed oocytes were treated with 500 nM Scriptaid for 14 to 16 h after post-fusion activation (6-DMAP/demecolcine). In our preliminary study, blastocyst rate significantly increased in the Sc-treated group, compared with the control group (25.1 ± 2.8% and 13.8 ± 1.9%, respectively, P < 0.05). We determined gene expression using quantitative real-time RT-PCR. The results showed that OCT3/4 gene was expressed at a similar level in in vivo and SCNT blastocysts with/without Sc. IGF2 and H19 genes tended to be highly expressed in both SCNT blastocysts with (1.6-fold and 3.1-fold, respectively) and without (2.0-fold and 5.8-fold, respectively) Sc than that of the in vivo blastocysts. We found differences in imprinted gene expression patterns between in vivo and cloned blastocysts. Expression of H19 and IGF2 in SCNT blastocysts after Scriptaid treatment decreased towards the expression levels of in vivo blastocysts. These results indicated that Scriptaid treatment in SCNT embryos may also have beneficial effects on in vitro developmental competence as well as their gene expression pattern.

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