scholarly journals Translesion Synthesis by DNA Polymerase θ Protects from Skin Cancers

2019 ◽  
Vol 9 (4) ◽  
pp. 463.1-463
2018 ◽  
Vol 8 (2) ◽  
pp. 754-754
Author(s):  
Likui Zhang ◽  
Yanchao Huang ◽  
Xinyuan Zhu ◽  
Yuxiao Wang ◽  
Haoqiang Shi ◽  
...  

2010 ◽  
Vol 2010 ◽  
pp. 1-10 ◽  
Author(s):  
Paromita Raychaudhury ◽  
Ashis K. Basu

-Radiation-induced intrastrand guanine-thymine cross-link, G[8,5-Me]T, hinders replicationin vitroand is mutagenic in mammalian cells. Herein we reportin vitrotranslesion synthesis of G[8,5-Me]T by human and yeast DNA polymerase (hPol and yPol ). dAMP misincorporation opposite the cross-linked G by yPol was preferred over correct incorporation of dCMP, but further extension was 100-fold less efficient for :A compared to :C. For hPol , both incorporation and extension were more efficient with the correct nucleotides. To evaluate translesion synthesis in the presence of all four dNTPs, we have developed a plasmid-based DNA sequencing assay, which showed that yPol was more error-prone. Mutational frequencies of yPol and hPol were 36% and 14%, respectively. Targeted was the dominant mutation by both DNA polymerases. But yPol induced targeted in 23% frequency relative to 4% by hPol . For yPol , targeted and constituted 83% of the mutations. By contrast, with hPol , semi-targeted mutations (7.2%), that is, mutations at bases near the lesion, occurred at equal frequency as the targeted mutations (6.9%). The kind of mutations detected with hPol showed significant similarities with the mutational spectrum of G[8,5-Me]T in human embryonic kidney cells.


DNA Repair ◽  
2008 ◽  
Vol 7 (1) ◽  
pp. 95-107 ◽  
Author(s):  
Yali Zhu ◽  
Liping Song ◽  
Jason Stroud ◽  
Deborah S. Parris

2001 ◽  
Vol 21 (1) ◽  
pp. 185-188 ◽  
Author(s):  
Sung-Lim Yu ◽  
Robert E. Johnson ◽  
Satya Prakash ◽  
Louise Prakash

ABSTRACT The yeast RAD30-encoded DNA polymerase η (Polη) bypasses a cis-syn thymine-thymine dimer efficiently and accurately. Human DNA polymerase η functions similarly in the bypass of this lesion, and mutations in human Polη result in the cancer prone syndrome, the variant form of xeroderma pigmentosum. UV light, however, also elicits the formation ofcis-syn cyclobutane dimers and (6-4) photoproducts at 5′-CC-3′ and 5′-TC-3′ sites, and in both yeast and human DNA, UV-induced mutations occur primarily by 3′ C to T transitions. Genetic studies presented here reveal a role for yeast Polη in the error-free bypass of cyclobutane dimers and (6-4) photoproducts formed at CC and TC sites. Thus, by preventing UV mutagenesis at a wide spectrum of dipyrimidine sites, Polη plays a pivotal role in minimizing the incidence of sunlight-induced skin cancers in humans.


2019 ◽  
Vol 84 (4) ◽  
pp. 1734-1747 ◽  
Author(s):  
Pratibha P. Ghodke ◽  
Praneeth Bommisetti ◽  
Deepak T. Nair ◽  
P. I. Pradeepkumar

Cell ◽  
2019 ◽  
Vol 176 (6) ◽  
pp. 1295-1309.e15 ◽  
Author(s):  
Jung-Hoon Yoon ◽  
Mark J. McArthur ◽  
Jeseong Park ◽  
Debashree Basu ◽  
Maki Wakamiya ◽  
...  

Author(s):  
Mathilde Fréchet ◽  
Corinne Cayrol ◽  
Christophe Cazaux ◽  
Nicolas Tanguy le Gac ◽  
Giuseppe Villani ◽  
...  

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