Analysis of the Cross–Reactivity between BtM and Der p 5, Two Group 5 Recombinant Allergens from Blomia tropicalis and Dermatophagoides pteronyssinus

1998 ◽  
Vol 117 (1) ◽  
pp. 38-45 ◽  
Author(s):  
Luis Caraballo ◽  
Dilia Mercado ◽  
Silvia Jiménez ◽  
Liliana Moreno ◽  
Leonardo Puerta ◽  
...  
Keyword(s):  
Dermatophagoides Pteronyssinus ◽  
Cross Reactivity ◽  
Recombinant Allergens ◽  
Blomia Tropicalis ◽  
The Cross ◽  
Group 5

Cross-Reactivity between IgE-Binding Proteins from Anisakis Simplex and Dermatophagoides Pteronyssinus

2005 ◽  
Vol 18 (4) ◽  
pp. 671-675 ◽  
Author(s):  
R. Bernardini ◽  
G. Mistrello ◽  
E. Novembre ◽  
D. Roncarolo ◽  
S. Zanotta ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Binding Proteins ◽  
Specific Ige ◽  
Dermatophagoides Pteronyssinus ◽  
Cross Reactivity ◽  
Anisakis Simplex ◽  
Cross Reaction ◽  
Ige Binding ◽  
Sds Page ◽  
The Cross

An association was found between Anisakis simplex (As) and Dermatophagoides pteronyssinus (Dp) sensitization. One recent study shows a cross-reactivity between As and Dp and tropomyosin (tr) is suspected as being one of the proteins responsible of this cross-reaction. The aim of our study was: 1) to confirm the cross-reactivity between Dp and As; 2) to determine the importance of tr in this cross reaction. SDS-PAGE analysis of Dp and As (metabolic and somatic) extracts was carried out. Then an IgE immunoblotting test using serum from a patient who had specific IgE only to Dp and As and immunoblotting inhibition experiments using Dp extract and tr as inhibitors were performed. We found that patient's serum reacted: 1) against larval As antigens with a molecular weight (mw) of 25 kilodalton (kD) and a mw > 100 kD, 2) against various metabolic As antigens with a mw > 100 kD, a mw ranging approximately from 35 to 50 kD, and a mw around 20 kD, and 3) against Dp proteins with mw between 35 and 55 kD. Preincubation of patient's serum with Dp extract caused the disappearance of reactivity against antigens with a mw > 100 kD in both larval and metabolic As extracts and against proteins with mw ranging approximately from 35 to 50 kD in the metabolic As extract. Preincubation of patient's serum with As extract caused the disappearance of reactivity against antigens with mw between 35 and 55 kD in the Dp extract. Pre-incubation of patient's serum with tr did not induce any change in the immunoblotting profile. The results show that 1) cross-reactive components between Dp and As are some proteins with a mw ranging approximately from 35 to 50 kD and with a mw > 100 kD, and 2) tr is not involved in cross-reactivity between As and Dp.

Cross-Reactivity between Major IgE Epitopes of Family 5 Allergens from Dermatophagoides pteronyssinus and Blomia tropicalis

2018 ◽  
Vol 178 (1) ◽  
pp. 10-18 ◽  
Author(s):  
Sadjia Lahiani ◽  
Marie-Eve Dumez ◽  
Souad Khemili ◽  
Idir Bitam ◽  
Dimitri Gilis ◽  
...  
Keyword(s):  
Dermatophagoides Pteronyssinus ◽  
Cross Reactivity ◽  
Blomia Tropicalis

scholarly journals Cross-reactivity between group-5 and -21 mite allergens from Dermatophagoides farinae, Tyrophagus putrescentiae and Blomia tropicalis

2015 ◽  
Vol 12 (4) ◽  
pp. 5467-5474 ◽  
Author(s):  
CHUNG-RYUL KIM ◽  
KYOUNG YONG JEONG ◽  
MYUNG-HEE YI ◽  
HYOUNG-PYO KIM ◽  
HO-JOON SHIN ◽  
...  
Keyword(s):  
Cross Reactivity ◽  
Dermatophagoides Farinae ◽  
Tyrophagus Putrescentiae ◽  
Blomia Tropicalis ◽  
Group 5

Cross-reactivity of rapid immunochemical methods for mycotoxins detection towards metabolites and masked mycotoxins: the current state of knowledge

2014 ◽  
Vol 7 (4) ◽  
pp. 449-464 ◽  
Author(s):  
M. Zachariasova ◽  
P. Cuhra ◽  
J. Hajslova
Keyword(s):  
Rapid Test ◽  
Cross Reactivity ◽  
Rapid Screening ◽  
Proficiency Tests ◽  
Assigned Value ◽  
Screening Practice ◽  
Current State ◽  
Immunochemical Methods ◽  
The Cross ◽  
Lateral Flow Test

The cross-reactivity of antibodies employed within immunochemistry-based analytical methods may lead to overestimation of the results. Under certain conditions, specifically when controlling mycotoxin maximum limits serious problems can be encountered. Not only the structurally related mycotoxins, such as their masked (conjugated) forms, but also the unidentified matrix components are responsible for concentration overestimation of respective target analytes. The cross-reactivity phenomenon may also pose a risk of miss-interpretation of the proficiency tests results, when the assigned value becomes influenced by over-estimated results reported by users of immunochemical tests. In this paper, the current state of the knowledge on trueness problems associated with the rapid screening immunochemical methods have been reviewed. Special attention is focused on discussion of cross-reactivity in the ELISA tests, because this rapid test dominates the routine screening practice. However, the cross-reactions reported in lateral flow test strips, fluorescence polarisation immunoassay, or immunosensors have also been addressed.

Immunity to tapeworms: intraspecific cross-protective interactions betweenHymenolepis citelli, H. diminutaandH. microstomain mice

Parasitology ◽  
1986 ◽  
Vol 92 (3) ◽  
pp. 665-674 ◽  
Author(s):  
Sidi T. O. Alghali ◽  
R. K. Grencis
Keyword(s):  
Primary Infection ◽  
Cross Reactivity ◽  
Significant Protection ◽  
Cyst Infection ◽  
Single Host ◽  
Concurrent Infections ◽  
Rejection Mechanism ◽  
The Cross

SUMMARYInteractions between tapeworm species in a single host offer intriguing opportunities for immunological studies that attempt to identify the mechanism(s) underlying protection against cestode infections. Mice that are immunized againstHymenolepis citelliinfections were shown to be refractory to subsequentH. diminutachallenge infections. The reciprocity of the response was also demonstrated, although the protection recorded forH. diminutawhen mice are sensitized withH. citelliis weaker than that observed when mice are primed withH. diminutaagainstH. citellichallenge.H. citelliwas also shown to be expelled simultaneously during the rejection phase ofH. diminutain concurrent infections, indicating the susceptibility of the former tapeworm to the rejection mechanism initiated by the latter.H. microstomaimmunized mice were shown to be strongly protected against heterologousH. citellichallenge. However, mice primed againstH. citelliwere not as strongly protected againstH. microstomachallenge infections: a statistically significant protection was obtained only after a 12-cysticercoidH. citelliprimary infection, although a 6-cyst infection did stunt the growth ofH. microstomachallenge worms. It is presently suggested that the cross-protective responses observed in the study betweenH. citelli, H. diminutaandH. microstomamay have emanated from a specific immunological cross-reactivity due to the sharing of similar immunogens.

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