Cross-Reactivity between Major IgE Epitopes of Family 5 Allergens from Dermatophagoides pteronyssinus and Blomia tropicalis

An association was found between Anisakis simplex (As) and Dermatophagoides pteronyssinus (Dp) sensitization. One recent study shows a cross-reactivity between As and Dp and tropomyosin (tr) is suspected as being one of the proteins responsible of this cross-reaction. The aim of our study was: 1) to confirm the cross-reactivity between Dp and As; 2) to determine the importance of tr in this cross reaction. SDS-PAGE analysis of Dp and As (metabolic and somatic) extracts was carried out. Then an IgE immunoblotting test using serum from a patient who had specific IgE only to Dp and As and immunoblotting inhibition experiments using Dp extract and tr as inhibitors were performed. We found that patient's serum reacted: 1) against larval As antigens with a molecular weight (mw) of 25 kilodalton (kD) and a mw > 100 kD, 2) against various metabolic As antigens with a mw > 100 kD, a mw ranging approximately from 35 to 50 kD, and a mw around 20 kD, and 3) against Dp proteins with mw between 35 and 55 kD. Preincubation of patient's serum with Dp extract caused the disappearance of reactivity against antigens with a mw > 100 kD in both larval and metabolic As extracts and against proteins with mw ranging approximately from 35 to 50 kD in the metabolic As extract. Preincubation of patient's serum with As extract caused the disappearance of reactivity against antigens with mw between 35 and 55 kD in the Dp extract. Pre-incubation of patient's serum with tr did not induce any change in the immunoblotting profile. The results show that 1) cross-reactive components between Dp and As are some proteins with a mw ranging approximately from 35 to 50 kD and with a mw > 100 kD, and 2) tr is not involved in cross-reactivity between As and Dp.

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Skin Tests and Conjunctival and Bronchial Challenges with Extracts of Blomia tropicalis and Dermatophagoides pteronyssinus in Patients with Allergic Asthma and/or Rhinoconjunctivitis

International Archives of Allergy and Immunology ◽

10.1159/000071484 ◽

2003 ◽

Vol 131 (3) ◽

pp. 182-188 ◽

Cited By ~ 13

Author(s):

J.C. García Robaina ◽

I. Sánchez Machín ◽

E. Fernández-Caldas ◽

V. Iraola Calvo ◽

C. Vázquez Moncholi ◽

...

Keyword(s):

Allergic Asthma ◽

Dermatophagoides Pteronyssinus ◽

Skin Tests ◽

Blomia Tropicalis

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A contribution to the fauna of mites (Acari) in Fiji

Systematic and Applied Acarology ◽

10.11158/saa.25.8.7 ◽

2020 ◽

Vol 25 (8) ◽

pp. 1444-1460

Author(s):

Qing-Hai Fan ◽

Shivana Sweta Dayal ◽

Hatch Michael Fong ◽

Panapasa Rakuita ◽

Jainesh Anish Ram

Keyword(s):

Dermatophagoides Pteronyssinus ◽

Blomia Tropicalis ◽

First Time

We recently had the opportunity to collect some mite samples in Viti Levu and Vanua Levu of Fiji and found a couple of dozens of species. In this paper we present 32 species in three orders—Mesostigmata: 15 species in 11 genera in 9 families; Sarcoptiformes: 13 species in 13 genera in 10 families; Trombidiformes: 4 species in 4 genera in 3 families. The following 22 species are recorded for the first time from Fiji: Ameroseius womersleyi in Ameroseiidae, Lasioseius cuppa, L. latinoamericanus, L. pellitus and L. penicilliger in Blattisociidae, Androlaelaps casalis in Laelapidae, Proctolaelaps aurora in Melicharidae, Propriosiopsis ovatus and Typhlodromus (Anthoseius) transvaalensis in Phytoseiidae; Funkotriplogynium ovulum in Triplogyniidae, Uroactinia hippocrepea in Uroactiniidae, Neotropacarus mumai and Schwiebea zingiberi in Acaridae, Glycycometus weelawadjiensis in Aeroglyphidae, Blomia tropicalis in Echimyopodidae, Pergalumna (P.) hawaiiensis hawaiiensis in Galumnidae, Nasozetes sumatrensis in Hemileiidae, Dermatophagoides pteronyssinus in Pyroglyphidae, Sudasia pontifica in Suidasiidae, Oulenziella bakeri in Winterschmidtiidae, Dactyloscirus smileyi in Cunaxidae, Tarsonemus rakowiensis in Tarsonemidae.

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