Immunohistochemical Localization of Cytochromes P450 with Polyclonal and Monoclonal Antibodies

1989 ◽  
Vol 8 (2) ◽  
pp. 61-94 ◽  
Author(s):  
Lucy M. Anderson ◽  
Jerrold M. Ward ◽  
Sang S. Park ◽  
Jerry M. Rice
Keyword(s):  
Monoclonal Antibodies ◽  
Cytochromes P450 ◽  
Immunohistochemical Localization

Immunohistochemical localization of S100 beta in human nervous system tumors by using monoclonal antibodies with specificity for the S100 beta polypeptide.

1986 ◽  
Vol 34 (8) ◽  
pp. 977-982 ◽  
Author(s):  
L J Van Eldik ◽  
R A Jensen ◽  
B A Ehrenfried ◽  
W O Whetsell
Keyword(s):  
Monoclonal Antibody ◽  
Nervous System ◽  
Monoclonal Antibodies ◽  
Calcium Binding ◽  
Binding Protein ◽  
Immunohistochemical Localization ◽  
Calcium Binding Protein ◽  
Nervous System Tumors ◽  
Human Nervous System

The immunohistochemical localization of the calcium-binding protein, S100 beta, in human nervous system tumors has been examined by using a monoclonal antibody with specificity for the S100 beta polypeptide. S100 beta-specific immunoreactivity is detected in astrocytoma, glioblastoma, Schwannoma, ependymoma, and craniopharyngioma, whereas no reactivity is seen in oligodendroglioma, meningioma, neuroblastoma, or medulloblastoma. These data suggest that analysis of S100 beta localization with these monoclonal antibodies may be useful for research or diagnostic purposes.

EFFECT OF CYTOCHROMES P450 CHEMICAL INHIBITORS AND MONOCLONAL ANTIBODIES ON HUMAN LIVER MICROSOMAL ESTERASE ACTIVITY

2006 ◽  
Vol 34 (8) ◽  
pp. 1361-1366 ◽  
Author(s):  
Stacey L. Polsky-Fisher ◽  
Hong Cao ◽  
Ping Lu ◽  
Christopher R. Gibson
Keyword(s):  
Monoclonal Antibodies ◽  
Esterase Activity ◽  
Human Liver ◽  
Cytochromes P450 ◽  
Chemical Inhibitors

scholarly journals Monoclonal antibodies against amyloid fibril protein AA. Production, specificity, and use for immunohistochemical localization and classification of AA-type amyloidosis.

1984 ◽  
Vol 32 (3) ◽  
pp. 322-328 ◽  
Author(s):  
R P Linke
Keyword(s):  
Monoclonal Antibodies ◽  
Amyloid Fibril ◽  
Immunohistochemical Localization ◽  
Immunoperoxidase Technique ◽  
Tissue Sections ◽  
Formalin Fixed Paraffin ◽  
Amyloid Fibril Protein ◽  
Indirect Immunoperoxidase ◽  
Hybridoma Cell Lines

Monoclonal antibodies against amyloid fibril protein AA were produced by cell fusion of murine P3 X 63-Ag8.653 myeloma cells with spleen cells of immunized Balb/c mice. To increase immunogenicity, protein AA was coupled to horseradish peroxidase (HRP) or human high molecular weight kininogen (HMWK). Using micro-ELISA (enzyme-linked immunosorbent essay) seven hybridoma cell lines secreting antibodies that specifically bind to protein AA have been selected and cloned. When applied to formalin-fixed paraffin sections of a variety of different amyloid types using immunoperoxidase methods, five monoclonal antibodies bound specifically and strongly to amyloid only of the AA type. Since a series of different AA-amyloids could be stained, these reagents may be used to routinely diagnose AA-amyloidosis in tissue sections. A monoclonal antibody against HRP has also been produced that has been utilized to develop a monoclonal peroxidase-antiperoxidases (PAP) complex. When three immunoperoxidase methods were compared, the sensitivity of a conventional rat PAP was comparable to the monoclonal PAP complex, but the latter was easier to handle. Both methods were more sensitive than the indirect immunoperoxidase technique.

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