Immunohistochemical Analysis of Pulmonary and Pleural Neoplasms Using a Monoclonal Antibody (47D10) Which Reacts with Nonspecific Cross-Reacting Antigen

SummaryGlycoprotein V (GPV) is a platelet membrane protein with a molecular weight of 82 kD, and one of the leucine rich glycoproteins (LRG). By reverse transcription-polymerase chain reaction (RT-PCR), GPV cDNA was amplified from mRNA of platelets and megakaryocytic cell lines. However, since there are few reports indicating whether GPV protein is expressed in megakaryocytes as a lineage and maturation specific protein, we studied the GPV expression at the protein level by using a novel monoclonal antibody (1D9) recognizing GPV. Flow cytometric and immunohistochemical analysis indicated that GPV was detected on the surface and in the cytoplasm of only the megakaryocytes in bone marrow aspirates. In a megakaryocytic cell line UT-7, GPV antigen increased after treatment with phorbol-12-myri-state-13-acetate (PMA). These data indicate that only megakaryocytes specifically express the GPV protein among hematopoietic cells and that the expression of GPV increases with differentiation of the megakaryocyte as GPIb-IX complex.

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PMab-213: A Monoclonal Antibody for Immunohistochemical Analysis Against Pig Podoplanin

Monoclonal Antibodies in Immunodiagnosis and Immunotherapy ◽

10.1089/mab.2018.0048 ◽

2019 ◽

Vol 38 (1) ◽

pp. 18-24 ◽

Cited By ~ 22

Author(s):

Yukinari Kato ◽

Shinji Yamada ◽

Yoshikazu Furusawa ◽

Shunsuke Itai ◽

Takuro Nakamura ◽

...

Keyword(s):

Monoclonal Antibody ◽

Immunohistochemical Analysis

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Cellular localization of adenosine A1 receptors in rat forebrain: Immunohistochemical analysis using adenosine A1 receptor-specific monoclonal antibody

The Journal of Comparative Neurology ◽

10.1002/(sici)1096-9861(19990823)411:2<301::aid-cne10>3.0.co;2-h ◽

1999 ◽

Vol 411 (2) ◽

pp. 301-316 ◽

Cited By ~ 81

Author(s):

Tomoyo Ochiishi ◽

Ling Chen ◽

Aya Yukawa ◽

Yoshiko Saitoh ◽

Yuko Sekino ◽

...

Keyword(s):

Monoclonal Antibody ◽

Cellular Localization ◽

Immunohistochemical Analysis ◽

Adenosine A1 Receptor ◽

Specific Monoclonal Antibody ◽

Adenosine A1 Receptors ◽

Adenosine A1 ◽

Rat Forebrain ◽

A1 Receptor

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Immunohistochemical analysis of rat liver using a monoclonal antibody (HAM8) against gap junction

The Anatomical Record ◽

10.1002/ar.1092350302 ◽

1993 ◽

Vol 235 (3) ◽

pp. 335-341 ◽

Cited By ~ 14

Author(s):

Yoshihisa Fujikura ◽

Hidehiko Ohta ◽

Toshio Hirai ◽

Tetsuo Fukumoto

Keyword(s):

Monoclonal Antibody ◽

Gap Junction ◽

Rat Liver ◽

Immunohistochemical Analysis

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Some monoclonal antibody reagents (C219 and JSB-1) to P-glycoprotein contain antibodies to blood group A carbohydrate determinants: a problem of quality control for immunohistochemical analysis.

Journal of Histochemistry & Cytochemistry ◽

10.1177/39.12.1682363 ◽

1991 ◽

Vol 39 (12) ◽

pp. 1603-1610 ◽

Cited By ~ 33

Author(s):

C L Finstad ◽

B W Yin ◽

C M Gordon ◽

M G Federici ◽

S Welt ◽

...

Keyword(s):

Monoclonal Antibody ◽

Blood Group ◽

Immunohistochemical Analysis ◽

Blood Type ◽

Polymorphic Variation ◽

Blood Group A ◽

P Glycoprotein ◽

Apparent Correlation ◽

Group A ◽

Type 3

Monoclonal antibodies (MAb) C219 and JSB-1 have been used extensively in the analysis of P-glycoprotein expression in normal and malignant tissues. This study demonstrates that some commercial lots of these MAb, even those supplied as purified immunoglobulins, contain contaminating anti-A blood group antibodies. In both sources of reagent, the antibody was specific for a particular A structure, known as repetitive or Type 3 A. These observations may account for earlier studies showing polymorphic variation in P-glycoprotein expression in epithelial tissues and an apparent correlation with the A blood type of the donor. Such reactivity can be eliminated by absorption of anti-P-glycoprotein reagents with A erythrocytes. These data re-emphasize the importance of evaluating MAb samples for unsuspected contaminating antibodies.

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