Regulating Influence of Transferred Immune Spleen Cells on the Primary Response of Mice to Sheep Red Blood Cells

Haemagglutination assay and haematological analysis of 143 poults generated as F1 individuals by artificial insemination from randomly selected turkeys of White, Black and Lavender genotypes which are classified by antibody titre was carried out so as to confirm their antibody titre levels in response to sheep red blood cells (SRBC). Results showed that mean values obtained for high and low antibody titres were 7.31 and 2.67 respectively, resulting in the classification of the turkeys into Black high and low, Lavender high and black, and White high and low antibody titres. The genotype’s titre had significant (P <0.05) effect on the packed cell volume (PCV), haemoglobin (Hb), red blood cells (RBC), white blood cells (WBC) and basophil (BAS) of the basal haematological parameters. Genotype’s titre had no significant (P >0.05) effect on the primary response haematological parameters. Meanwhile, the primary response haematological parameters to SRBC antigen varied along the genotypes with the WBC increasing drastically in all the genotypes, signifying the presence of an antigen. The study concluded that the F1 turkey poult population studied diverged along the high and low antibody titre in response to SRBC. Thus, the F1 generation of the high antibody titre genotypes (Black high, White high and Lavender high) can be used as foundation stock for selection of local turkeys for high antibody titre.

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Influence of Brucella Endotoxins on the Initiation of Antibody-Forming Spleen Cells in Mice Immunized with Sheep Red Blood Cells

Infection and Immunity ◽

10.1128/iai.2.1.1-6.1970 ◽

1970 ◽

Vol 2 (1) ◽

pp. 1-6 ◽

Cited By ~ 5

Author(s):

Gérard Renoux ◽

Micheline Renoux ◽

Régina Tinelli

Keyword(s):

Red Blood Cells ◽

Blood Cells ◽

Spleen Cells ◽

Sheep Red Blood Cells

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Structure of the lymphocyte membrane. II. Evidence of two classes of Ig on the surface of rabbit lymphocytes during primary response to sheep red blood cells using inhibition of rosette-forming cells and combined ultrastructural autoradiographic and peroxydase methods

European Journal of Immunology ◽

10.1002/eji.1830020510 ◽

1972 ◽

Vol 2 (5) ◽

pp. 434-439 ◽

Cited By ~ 15

Author(s):

C. A. Bona ◽

I. Trebiciavsky ◽

Anny Anteunis ◽

C. Heuclin ◽

R. Robineaux

Keyword(s):

Red Blood Cells ◽

Blood Cells ◽

Primary Response ◽

Lymphocyte Membrane ◽

Sheep Red Blood Cells ◽

Rabbit Lymphocytes

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THE EFFECT OF HETEROLOGOUS ANTI-LYMPHOCYTE SERUM ON LYMPHOCYTES OF THYMUS AND MARROW ORIGIN

Journal of Experimental Medicine ◽

10.1084/jem.132.5.963 ◽

1970 ◽

Vol 132 (5) ◽

pp. 963-975 ◽

Cited By ~ 14

Author(s):

H. F. Jeejeebhoy

Keyword(s):

Red Blood Cells ◽

Spleen Cell ◽

Blood Cells ◽

Synergistic Interaction ◽

Cell Populations ◽

Spleen Cells ◽

Final Experiment ◽

Sheep Red Blood Cells ◽

Thymus Cells ◽

Marrow Cells

When CY-treated mice were given sheep red blood cells the serum hemagglutinin titers produced were significantly lower than those found when mice received SRBC but not CY. Titers could be raised to the levels found in the latter group if, in addition to SRBC, the CY-treated mice received 2 x 107 normal syngeneic spleen cells or a mixture containing 1.2 x 108 normal thymus and 1.2 x 108 normal marrow cells. Inocula which contained fewer cells produced correspondingly smaller amounts of antibody. A synergistic interaction between normal thymus and marrow cells was always demonstrable in these experiments. Hemagglutinin titers produced by CY-treated mice given SRBC and 2 x 107 normal syngeneic spleen cells were always much higher than those found when the spleen cells were obtained from animals previously given ALS. Titers could be raised to normal levels if the animals in this latter group received additional injections containing mixtures of normal syngeneic thymus and marrow cells. Marrow cells alone were completely ineffective, while inocula which only contained thymus cells were much less effective than mixtures of thymus and marrow cells. These results suggest that immunosuppression by ALS is associated with the inactivation of both thymus and marrow-derived lymphocytes. In other experiments CY-treated mice received SRBC and mixtures of thymus and marrow cells from both untreated and ALS-treated donors. No decrease in the immunological competence of cells located within the thymus of ALS-treated donors was demonstrable in these experiments. Marrow cells were slightly affected but to a markedly lesser degree than were spleen cells of ALS-treated animals. In a final experiment, it was possible to show that the thymus and marrow cells of ALS-treated animals could repair the immunological defects which were present in their own spleen cell populations.

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