scholarly journals Antibody Response and Haematological Changes of Nigerian Locally Adapted Turkeys to Sheep Red Blood Cells (SRBC)

Author(s):  
Ayodele Emmanuel OGUNDERO ◽  
Mofoyeke Oluwayemisi SANDA ◽  
Adeyemi Sunday ADENAIKE ◽  
Michael Irewole TAKEET ◽  
Christian Obiora Ndubuisi IKEOBI

Haemagglutination assay and haematological analysis of 143 poults generated as F1 individuals by artificial insemination from randomly selected turkeys of White, Black and Lavender genotypes which are classified by antibody titre was carried out so as to confirm their antibody titre levels in response to sheep red blood cells (SRBC). Results showed that mean values obtained for high and low antibody titres were 7.31 and 2.67 respectively, resulting in the classification of the turkeys into Black high and low, Lavender high and black, and White high and low antibody titres. The genotype’s titre had significant (P <0.05) effect on the packed cell volume (PCV), haemoglobin (Hb), red blood cells (RBC), white blood cells (WBC) and basophil (BAS) of the basal haematological parameters. Genotype’s titre had no significant (P >0.05) effect on the primary response haematological parameters. Meanwhile, the primary response haematological parameters to SRBC antigen varied along the genotypes with the WBC increasing drastically in all the genotypes, signifying the presence of an antigen. The study concluded that the F1 turkey poult population studied diverged along the high and low antibody titre in response to SRBC. Thus, the F1 generation of the high antibody titre genotypes (Black high, White high and Lavender high) can be used as foundation stock for selection of local turkeys for high antibody titre.

Author(s):  
RaianBakhet Yassein ◽  
Nada Omer Alseedig ◽  
SihamKhalifaAbd Allah ◽  
AreegAlsail Mohmmed ◽  
NosibaAbdelmajid Alballah ◽  
...  

Chronic renal failure is one of the more common causes of morbidity and mortality among Sudanese. This study describes altered haemoglobin concentration (Hb), haematochrit percent (Hct), red blood cells count (RBCs), platelet count (Plts) and white blood cells count (WBCs) in patients with chronic renal failure and describes the effect of disease duration on haemoglobin concentration and red blood cells count. Methods: Haematological parameters in (50) patients with Chronic renal failure and (50) age and sex matched controls were evaluated, living in Shendi locality in northern Sudan. The results of this study showed that mean of Hb concentration, RBCs count, Hct percent and platelet count were exhibited significant decreased, but mean of WBCs count was insignificantly increased. Increased duration of the disease lead to significantly decreased on mean of Hb concentration and RBCs count. Chronic renal failure is associated with different degrees of reduced haematological parameters. Also increase duration of the disease lead to significantly decrease in mean of Hb concentration and RBCs count.


Author(s):  
U. O. Ozojiofor ◽  
O. O. Bankole ◽  
N. Anene ◽  
A. U. Hassan ◽  
S. A. Emaleku

The present study was carried to determine the changes in haematological parameters in P. falciparum infected patients in Ajeromi Ifelodun area of Lagos, Nigeria. Seventy (70) human subjects comprising of 50 P. falciparum malarial infected and 20 non-infected (control) subjects between 10-60 years were selected for this study. RDT test and microscopy were carried out to ascertain the presence of P. falciparum. They were grouped based on age criteria and level of parasitaemia. This work was carried out in the Department of Biochemistry, Nigeria Institute for Medical Research Laboratory, Yaba, Lagos, Nigeria between August 2016 and January 2017. Blood samples were collected for the determination of P. falciparum, level of parasitaemia and haematological parameters. Haematological parameters were determined using a Coulter A-T Pierce haematology analyzer (Beckman Coulter, Inc. Fullerton, CA, USA), P. falciparum was determined by rapid diagnostic test (RDT) and Microscopy. There was a significant increase in the mean level of total white blood cells (WBC) and red blood cells distribution width (RDW), and a significant decrease in the mean level of haematocrit (HCT), haemoglobin (HGB), red blood cells (RBC), and platelets (PLT) in the malaria infected patients than in the controls (p<0.05). There was also a higher malaria parasite density among malaria infected patients for ages above 20 and a lower malaria parasite density for ages below 20 in this study. The findings of this study show that infection with P. falciparum produces changes in haematological parameters in those infected and tested positive for malaria. The most commonly affected parameters are haemoglobin, haematocrit, white blood cells and platelet count.


2017 ◽  
Vol 58 (2) ◽  
pp. 124 ◽  
Author(s):  
N. K. PANOUSIS (Ν.Κ. ΠΑΝΟΥΣΗΣ) ◽  
M. KRITSEPI-KONSTANTINOU (Μ. ΚΡΙΤΣΕΠΗ-ΚΩΝΣΤΑΝΤΙΝΟΥ) ◽  
N. D. GIADINIS (Ν.Δ. ΓΙΑΔΙΝΗΣ) ◽  
E. KALAITZAKIS (Ε. ΚΑΛΑΪΤΖΑΚΗΣ) ◽  
Z. POLIZOPOULOU (Ζ. ΠΟΛΥΖΟΠΟΥΛΟΥ) ◽  
...  

The main aim of the present study was to determine the values of the haematological parameters of dairy sheep reared under intensive and semi-intensive conditions, as well as to test for the effects of age and reproductive stage of the animals on the values of these parameters. In total, 200 clinically healthy Chios sheep from 10 farms of Central Macedonia were used in the study. For the determination of the effect of age 150 sheep were assigned in three groups. Group A consisted of 50 lambs aged 3-6 months (meantSD: 4.45±1.22 months), group Β of 50 non-pregnant ewes into lactation aged 1-3 years (meantSD: 2.18±0.74 years) and group C of 50 non-pregnant ewes into lactation aged more than 3 years (meantSD: 5.88±1.87 years). For evaluating the effect of reproductive status 50 pregnant ewes in dry period were used, 15-30 days before the expected day of lambing (group D), along with the 100 non-pregnant ewes into lactation of groups Β and C (group E). Blood sampling was performed once, in dry ewes from December to January, and in lambs and lactating ewes from March to May. The mean values of the determined haematological parameters were within the range provided by the literature, except for red blood cells count of ewes. The results also showed that haematocrit, haemoglobin concentration and red blood cells, white blood cells, neutrophils, lymphocytes, eosinophils and platelets count were significantly affected by the age; only monocytes and eosinophils count by the reproductive stage of the animals.


1990 ◽  
Vol 63 (01) ◽  
pp. 112-121 ◽  
Author(s):  
David N Bell ◽  
Samira Spain ◽  
Harry L Goldsmith

SummaryThe effect of red blood cells, rbc, and shear rate on the ADPinduced aggregation of platelets in whole blood, WB, flowing through polyethylene tubing was studied using a previously described technique (1). Effluent WB was collected into 0.5% glutaraldehyde and the red blood cells removed by centrifugation through Percoll. At 23°C the rate of single platelet aggregtion was upt to 9× greater in WB than previously found in platelet-rich plasma (2) at mean tube shear rates Ḡ = 41.9,335, and 1,920 s−1, and at both 0.2 and 1.0 µM ADP. At 0.2 pM ADP, the rate of aggregation was greatest at Ḡ = 41.9 s−1 over the first 1.7 s mean transit time through the flow tube, t, but decreased steadily with time. At Ḡ ≥335 s−1 the rate of aggregation increased between t = 1.7 and 8.6 s; however, aggregate size decreased with increasing shear rate. At 1.0 µM ADP, the initial rate of single platelet aggregation was still highest at Ḡ = 41.9 s1 where large aggregates up to several millimeters in diameter containing rbc formed by t = 43 s. At this ADP concentration, aggregate size was still limited at Ḡ ≥335 s−1 but the rate of single platelet aggregation was markedly greater than at 0.2 pM ADP. By t = 43 s, no single platelets remained and rbc were not incorporated into aggregates. Although aggregate size increased slowly, large aggregates eventually formed. White blood cells were not significantly incorporated into aggregates at any shear rate or ADP concentration. Since the present technique did not induce platelet thromboxane A2 formation or cause cell lysis, these experiments provide evidence for a purely mechanical effect of rbc in augmenting platelet aggregation in WB.


1980 ◽  
Vol 21 (2) ◽  
pp. 291-298
Author(s):  
Veslemøy Myhruold

Author(s):  
A. Abilov ◽  
A. Azhmyakov ◽  
I. Novgorodova ◽  
N. Bogolyubova

Purpose: to study hematological parameters of blood in bulls-producers of dairy breeds on the day of semen collection in the Udmurt Republic after a long winter period of operation, depending on the breeds, age and place of selection.Materials and methods. The work was performed at the Federal Research Center for Animal Husbandry named after Academy Member L. K. Ernst on the basis of AO "Udmurtplem" of the Udmurt Republic in the period from 2020 to 2021 on dairy bulls (n=20) aged 15-69 months, including a purebred Holstein breed of domestic selection (n=6), a Holstein black-and-white breed of European selection (the Netherlands, n=6), a black-and-white breed with blood transfusion on Holsteins on at the level of 94-98% (n=8). The content of white blood cells, red blood cells, hemoglobin and hematocrit, depending on age and breed, was studied on the ABC VET hematological analyzer on the day of taking the seed.Results. It was found that on average, in 20 bulls aged 15-69 months, the level of white blood cells was at the level of reference values of 8.8±0.25 x 109/l, red blood cells 10.3 x 1012/l, which is 50% more than the reference values. The concentration of hemoglobin is 128.0±2.92 g/l, hematocrit is on average 54.3%, with a norm of 24-46%. Breeding bulls at a reliable level, differing in age, showed that some animals had high indicators for red blood cells of 10.3±0.26 x 102, for hematocrit of 54.3% against 24-46% of reference values. Also, according to hematological indicators, there was a tendency to increase the concentration of hemoglobin and hematocrit in European-bred bulls.Conclusion. The study of the variability in hematological parameters depending on the selection showed that there is no significant difference in leukocytes and all indicators are at the level of reference values, and in erythrocytes more than 50% than the highest indicators. The highest hemoglobin values were at the level of 141-156 g/l instead of 128 g/l according to the highest reference values. Hematocrit also showed high max values in all groups in comparison with the reference values of 59-66% versus 46% in the norm. It is necessary when analyzing hematological blood parameters in addition to the average statistical indicators (M+m) also, monitor the variability (min-max) in order to obtain more objective information.


Sign in / Sign up

Export Citation Format

Share Document