A. In vivo Diffusion Chamber Culture Methodology

2015 ◽  
pp. 321-365
Author(s):  
Arland L. Carsten
Keyword(s):  
Diffusion Chamber ◽  
Diffusion Chamber Culture

scholarly journals In vivo induction of granulopoiesis in visceral yolksac cells by foetal hepatic factors

Development ◽  
1983 ◽  
Vol 73 (1) ◽  
pp. 87-95
Author(s):  
M. A. Anckaert ◽  
M. Symann
Keyword(s):  
Stem Cells ◽  
Double Diffusion ◽  
Diffusion Chamber ◽  
Yolk Sac ◽  
Hepatic Tissue ◽  
System A ◽  
Visceral Yolk Sac ◽  
Diffusion Chamber Culture ◽  
Adult Bone

In order to evaluate the hypothetical activity of foetal hepatic factors on putative yolk-sac haemopoietic stem cells we used the Double Diffusion Chamber (DDC) technique. The DDC were made of a regulator compartment, where foetal hepatic tissue was introduced and a test compartment where visceral yolk-sac cells were cultured. In this system a hepatic signal induced the yolk-sac stem cells to differentiate along the granulocytic pathway but did not stimulate yolk-sac CFUs growth. Contrary to CFUs originating from foetal liver or adult bone marrow, yolk-sac CFUs do not increase numerically in diffusion chamber culture.

scholarly journals Assessment of erythrocytic and granulocytic colony formation in an in vivo plasma clot diffusion chamber culture system

Blood ◽  
1976 ◽  
Vol 47 (6) ◽  
pp. 1041-1051
Author(s):  
HN Steinberg ◽  
ES Handler ◽  
EE Handler
Keyword(s):  
Colony Formation ◽  
Bone Marrow Cells ◽  
Secondary Growth ◽  
Diffusion Chamber ◽  
Plasma Clot ◽  
Granulocytic Colony ◽  
Normal Rat ◽  
Diffusion Chamber Culture ◽  
Marrow Cells

Normal rat bone marrow cells seeded into a plasma clot diffusion chamber culture developed into erythrocytic and granulocytic colonies in vivo. Chambers implanted into the peritoneal cavity of normal hosts showed erythrocytic colony numbers reaching an initial peak on day 2, declining on days 3--5, and increasing in a secondary growth phase on day 7. Day 2 colonies were evenly dispersed; day 7 colonies were grouped into discrete areas of bursts. Granulocytic colony numbers reached a peak on day 4 and gradually declined through day 7. Cells in various stages of differentiation could be detected in both colony types. Colony numbers were proportional to the number of marrow cells seeded into the chamber. Host animals treated with phenylhydrazine induced a marked increase in erythrocytic colony numbers and size and a decrease in granulocytic colony formation. Host animals treated with endotoxin suppressed erythrocytic colonies while increasing granulocytic colony size. This method may prove advantageous for the study of hematopoietic colony formation in a physiologic environment.

scholarly journals Potentiality of the murine colony-forming cells detected by an in vivo diffusion chamber culture system

Blood ◽  
1985 ◽  
Vol 66 (3) ◽  
pp. 686-689
Author(s):  
E Niskanen ◽  
HE Wyandt
Keyword(s):  
Bone Marrow ◽  
Colony Formation ◽  
Culture System ◽  
Bone Marrow Cells ◽  
Diffusion Chamber ◽  
Diffusion Chambers ◽  
Hemopoietic Cells ◽  
Chromosome Marker ◽  
Diffusion Chamber Culture

Culture of a mixture of bone marrow cells with and without T6 chromosome marker in diffusion chambers in mice yielded colonies (CFU- DG) containing cells of a single karyotype, suggesting clonality. Injection of individual CFU-DG colonies into lethally irradiated mice resulted in increased spleen colony formation on day 12 (CFU-S). The possibility of endogenous origin was excluded by demonstrating the presence of T6 marker in both CFU-DG and CFU-S colonies in karyotypically normal hosts. These findings suggest that the cells giving rise to granulocytic colonies in diffusion chambers also can give rise to multipotential hemopoietic cells.

scholarly journals Assessment of erythrocytic and granulocytic colony formation in an in vivo plasma clot diffusion chamber culture system

Blood ◽  
1976 ◽  
Vol 47 (6) ◽  
pp. 1041-1051 ◽  
Author(s):  
HN Steinberg ◽  
ES Handler ◽  
EE Handler
Keyword(s):  
Colony Formation ◽  
Bone Marrow Cells ◽  
Secondary Growth ◽  
Diffusion Chamber ◽  
Plasma Clot ◽  
Granulocytic Colony ◽  
Normal Rat ◽  
Diffusion Chamber Culture ◽  
Marrow Cells

Abstract Normal rat bone marrow cells seeded into a plasma clot diffusion chamber culture developed into erythrocytic and granulocytic colonies in vivo. Chambers implanted into the peritoneal cavity of normal hosts showed erythrocytic colony numbers reaching an initial peak on day 2, declining on days 3--5, and increasing in a secondary growth phase on day 7. Day 2 colonies were evenly dispersed; day 7 colonies were grouped into discrete areas of bursts. Granulocytic colony numbers reached a peak on day 4 and gradually declined through day 7. Cells in various stages of differentiation could be detected in both colony types. Colony numbers were proportional to the number of marrow cells seeded into the chamber. Host animals treated with phenylhydrazine induced a marked increase in erythrocytic colony numbers and size and a decrease in granulocytic colony formation. Host animals treated with endotoxin suppressed erythrocytic colonies while increasing granulocytic colony size. This method may prove advantageous for the study of hematopoietic colony formation in a physiologic environment.

scholarly journals Potentiality of the murine colony-forming cells detected by an in vivo diffusion chamber culture system

Blood ◽  
1985 ◽  
Vol 66 (3) ◽  
pp. 686-689 ◽  
Author(s):  
E Niskanen ◽  
HE Wyandt
Keyword(s):  
Bone Marrow ◽  
Colony Formation ◽  
Culture System ◽  
Bone Marrow Cells ◽  
Diffusion Chamber ◽  
Diffusion Chambers ◽  
Hemopoietic Cells ◽  
Chromosome Marker ◽  
Diffusion Chamber Culture

Abstract Culture of a mixture of bone marrow cells with and without T6 chromosome marker in diffusion chambers in mice yielded colonies (CFU- DG) containing cells of a single karyotype, suggesting clonality. Injection of individual CFU-DG colonies into lethally irradiated mice resulted in increased spleen colony formation on day 12 (CFU-S). The possibility of endogenous origin was excluded by demonstrating the presence of T6 marker in both CFU-DG and CFU-S colonies in karyotypically normal hosts. These findings suggest that the cells giving rise to granulocytic colonies in diffusion chambers also can give rise to multipotential hemopoietic cells.

Propagation of Rauscher Leukemia Virus to Human Lymphoblastoid Cells by in Vivo Diffusion Chamber Cultivation

1973 ◽  
Vol 143 (3) ◽  
pp. 850-853
Author(s):  
I. Miyoshi ◽  
S. Yoshimoto ◽  
T. Tsubota ◽  
S. Fujiwara ◽  
H. Dabasaki ◽  
...  
Keyword(s):  
Leukemia Virus ◽  
Diffusion Chamber ◽  
Lymphoblastoid Cells ◽  
Rauscher Leukemia ◽  
Rauscher Leukemia Virus
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