Immunocytochemical analysis of cellular components in atherosclerotic lesions. Use of monoclonal antibodies with the Watanabe and fat-fed rabbit.

1986 ◽  
Vol 6 (6) ◽  
pp. 601-613 ◽  
Author(s):  
T Tsukada ◽  
M Rosenfeld ◽  
R Ross ◽  
A M Gown
Keyword(s):  
Monoclonal Antibodies ◽  
Immunocytochemical Analysis ◽  
Atherosclerotic Lesions ◽  
Cellular Components

scholarly journals Monoclonal Antibodies Recognizing Atherosclerotic Lesions in WHHL Rabbit

1986 ◽  
Vol 2 ◽  
pp. 20-23
Author(s):  
Tatsuya TAKANO ◽  
Keiji NAKAGAMI ◽  
Junji KIMURA ◽  
Nobuoki HAYASHI ◽  
Shoji OHKUMA
Keyword(s):  
Monoclonal Antibodies ◽  
Atherosclerotic Lesions ◽  
Whhl Rabbit

scholarly journals Agrin-like molecules at synaptic sites in normal, denervated, and damaged skeletal muscles.

1987 ◽  
Vol 105 (6) ◽  
pp. 2457-2469 ◽  
Author(s):  
N E Reist ◽  
C Magill ◽  
U J McMahan
Keyword(s):  
Monoclonal Antibodies ◽  
Neuromuscular Junction ◽  
Basal Lamina ◽  
Skeletal Muscles ◽  
Neuromuscular Junctions ◽  
Electric Organ ◽  
Synaptic Cleft ◽  
Cellular Components ◽  
Term Maintenance

Several lines of evidence have led to the hypothesis that agrin, a protein extracted from the electric organ of Torpedo, is similar to the molecules in the synaptic cleft basal lamina at the neuromuscular junction that direct the formation of acetylcholine receptor and acetylcholinesterase aggregates on regenerating myofibers. One such finding is that monoclonal antibodies against agrin stain molecules concentrated in the synaptic cleft of neuromuscular junctions in rays. In the studies described here we made additional monoclonal antibodies against agrin and used them to extend our knowledge of agrin-like molecules at the neuromuscular junction. We found that anti-agrin antibodies intensely stained the synaptic cleft of frog and chicken as well as that of rays, that denervation of frog muscle resulted in a reduction in staining at the neuromuscular junction, and that the synaptic basal lamina in frog could be stained weeks after degeneration of all cellular components of the neuromuscular junction. We also describe anti-agrin staining in nonjunctional regions of muscle. We conclude the following: (a) agrin-like molecules are likely to be common to all vertebrate neuromuscular junctions; (b) the long-term maintenance of such molecules at the junction is nerve dependent; (c) the molecules are, indeed, a component of the synaptic basal lamina; and (d) they, like the molecules that direct the formation of receptor and esterase aggregates on regenerating myofibers, remain associated with the synaptic basal lamina after muscle damage.

3.P.157 Role of the thymus in experimental atherogenesis in cholesterol-fed rabbits: Immunocytochemical analysis of cellular components

1997 ◽  
Vol 134 (1-2) ◽  
pp. 231
Author(s):  
H. Honda ◽  
Y. Fukuo ◽  
K. Akimaru ◽  
H. Katayama ◽  
K. Kameyama ◽  
...  
Keyword(s):  
Immunocytochemical Analysis ◽  
Cellular Components

scholarly journals Canine Lymphoma: Immunocytochemical Analysis of Fine-needle Aspiration Biopsy

1996 ◽  
Vol 33 (2) ◽  
pp. 204-212 ◽  
Author(s):  
M. Caniatti ◽  
P. Roccabianca ◽  
E. Scanziani ◽  
S. Paltrinieri ◽  
P. F. Moore
Keyword(s):  
Monoclonal Antibodies ◽  
Polyclonal Antibody ◽  
Fine Needle Aspiration ◽  
Fine Needle Aspiration Biopsy ◽  
Surface Antigens ◽  
Needle Aspiration ◽  
Canine Lymphoma ◽  
Fine Needle ◽  
Immunocytochemical Analysis ◽  
Fine Needle Aspirates

Cytospin preparations of fine-needle aspirates from 21 dogs with peripheral lymphadenopathy (18 with lymphoma and three with lymph node hyperplasia) were studied by combining morphologic and immunocytochemical analysis. Fine-needle aspirates were taken from at least two enlarged lymph nodes, and the diagnosis was based on air-dried smears stained with May-Grünwald Giemsa. Fine-needle aspiration biopsy always provided an adequate quality and quantity of cells to perform morphologic and immunologic studies. Immunophenotyping was performed on cytospin preparations with a panel of eight monoclonal antibodies specific for canine cell surface antigens and one rabbit polyclonal antibody (A452) against human CD3, which cross-reacts with dog antigen. The immunocytochemical study resulted in the diagnosis of 14 B-cell lymphomas (CD21 +, CD3-) and three T-cell lymphomas (all CD3 +, two CD8+). One lymphoma lacked surface antigens specific for the B- or T-cell lineage and was classified as non-B-non-T lymphoma (CD21-, CD3-, CD4-, CD8-). The monoclonal antibodies CA12.10C12, CA4.1D3, and CA1D6 and the polyclonal antibody A452, used as a group, appeared to be the most useful reagents to suggest lymphoid origin and to discriminate between T- and B-cell phenotype. Cytospin preparations in combination with immunocytochemistry provided a practical, economical, and accurate method for the diagnosis and phenotyping of canine lymphoma.

scholarly journals Protease receptors in Hodgkin's disease: expression of the factor Xa receptor, effector cell protease receptor-1, in Reed-Sternberg cells

Blood ◽  
1996 ◽  
Vol 88 (4) ◽  
pp. 1457-1464 ◽  
Author(s):  
C Adida ◽  
G Ambrosini ◽  
J Plescia ◽  
PL Crotty ◽  
J Costa ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Hodgkin's Disease ◽  
Effector Cell ◽  
Hodgkin’S Disease ◽  
Factor Xa ◽  
Immunohistochemical Analysis ◽  
Tissue Samples ◽  
Hodgkin's Lymphomas ◽  
Cellular Components

The expression of a cellular receptor for the blood-clotting protease factor Xa, designated effector cell protease receptor-1 (EPR-1), was investigated in lymphoma. Immunohistochemical analysis demonstrated prominent reactivity of monoclonal antibodies to EPR-1 with Reed- Sternberg cells in 30 of 35 cases of nodular-sclerosis, lymphocyte- depletion, and mixed-cellularity Hodgkin's disease (HD). In contrast, several non-Hodgkin's lymphomas, or the nonneoplastic cellular components of HD, did not react with anti-EPR-1 monoclonal antibodies. A single molecular species of approximately 62 kD, consistent with the size and structural organization of EPR-1, was immunoblotted by an anti- EPR-1 monoclonal antibody from tissue samples of HD, but not from normal lymph nodes. Expression of EPR-1 transcripts in Reed-Sternberg cells was demonstrated by in situ hybridization with an antisense EPR-1 riboprobe, and by amplification of reverse-transcribed HD RNA with EPR- 1-specific primers. These findings identify the factor Xa receptor, EPR- 1, as a novel marker of Reed-Sternberg cells, and suggest its potential role in the histopathogenesis of HD.

scholarly journals Monoclonal Antibodies Recognizing Atherosclerotic Lesions

1996 ◽  
Vol 23 (6) ◽  
pp. 351-355
Author(s):  
Tsuneo IMANAKA ◽  
Hiroyuki ITABE ◽  
Tatsuya TAKANO
Keyword(s):  
Monoclonal Antibodies ◽  
Atherosclerotic Lesions

Monoclonal antibodies against LDL progressively oxidized by myeloperoxidase react with ApoB-100 protein moiety and human atherosclerotic lesions

2004 ◽  
Vol 323 (4) ◽  
pp. 1223-1228 ◽  
Author(s):  
N. Moguilevsky ◽  
K. Zouaoui Boudjeltia ◽  
S. Babar ◽  
P. Delrée ◽  
I. Legssyer ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Atherosclerotic Lesions ◽  
Protein Moiety
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