scholarly journals Spiral Waves and Reentry Dynamics in an In Vitro Model of the Healed Infarct Border Zone

2009 ◽  
Vol 105 (11) ◽  
pp. 1062-1071 ◽  
Author(s):  
Marvin G. Chang ◽  
Yibing Zhang ◽  
Connie Y. Chang ◽  
Linmiao Xu ◽  
Roland Emokpae ◽  
...  
Keyword(s):  
In Vitro Model ◽  
Spiral Waves ◽  
Border Zone ◽  
Vitro Model ◽  
Infarct Border Zone ◽  
Infarct Border

Abstract 1096: Cytokine Enhancement with Hgf Or Vegf in the Infarct Border Zone is Key to Attenuating the Negative Remodelling after Myocardial Infarction

Circulation ◽  
2008 ◽  
Vol 118 (suppl_18) ◽  
Author(s):  
Sonja Schrepfer ◽  
Tobias Deuse ◽  
Christoph Peter ◽  
William Stein ◽  
Tim Doyle ◽  
...  
Keyword(s):  
Myocardial Infarction ◽  
Treatment Strategies ◽  
Border Zone ◽  
Cytokine Release ◽  
Intramyocardial Injection ◽  
Paracrine Effects ◽  
Infarct Border Zone ◽  
Infarct Border

Adult mesenchymal stem cell (MSC)-based treatment strategies have been proposed to alleviate the consequences of myocardial infarction (MI). The cytokine release of ischemic myocardium was investigated in vivo after LAD ligations in mice and in vitro in cultured cardiomyocytes. Of all cytokines that were at least 5-fold upregulated during ischemia, only HGF and VEGF proved to promote MSC proliferation, and chemotaxis in vitro. Homing of intranenously (IV) injected MSCs (0.5×106 per animal) into the infarct border zone after LAD ligation was inefficient (1±0.5 cells/HPF). Cytokine enhancement (CE) of HGF or VEGF by intramyocardial injection at the time of MI significantly facilitated MSC homing (11±4 cells/HPF and 7±4 cells/HPF, respectively; p=0.001). To our knowledge, this is the first study monitoring cardiac geometry and function over a long-term period of 6 months. using ECG-triggered contrast Micro-CT. It revealed that the progressive decrease in EF over time (to 19±1%) could be attenuated by CE with HGF (29±6%; p=0.003) or VEGF (28±4%; p=0.004) and subsequent IV MSC injection. However, LVEFs of animals treated with CE with HGF or VEGF only, but received no MSC injection, were similar to those groups that also received IV MSCs (p=0.127 and p=0.54, respectively). Best results were finally achieved by prolonged presence of HGF or VEGF, achieved by intramyocardial injection of MSCs stably transfected to produce HGF or VEGF and firefly luciferase into the infarct border zone. Duration of cytokine release was estimated by monitoring MSC survival using in vivo bioluminescence imaging (BLI). BLI signals were detectable for 10 days in contrast to the rapid fate of the cytokines after single dose administration in the CE group, resulting in preserved LVEFs at 6 months This study highlights the beneficial effect of HGF and VEGF to attenuate the negative LV remodelling after MI and diminishes the role of the MSCs to a pure delivery system for paracrine effects.

scholarly journals Electrophysiological Effects of Dofetilide in an In Vitro Model of “Border Zone” Between Normal and Ischemic/Reperfused Myocardium

Circulation ◽  
2000 ◽  
Vol 101 (1) ◽  
pp. 86-93 ◽  
Author(s):  
René Rouet ◽  
Sandra Picard ◽  
Christian Libersa ◽  
Mathieu Ghadanfar ◽  
Colin Alabaster ◽  
...  
Keyword(s):  
In Vitro Model ◽  
Border Zone ◽  
Vitro Model ◽  
Electrophysiological Effects ◽  
Reperfused Myocardium

Dronedarone Versus Amiodarone in Preventing Premature Ventricular Contractions in an In Vitro Model of “Border Zone”

2014 ◽  
Vol 63 (1) ◽  
pp. 49-57 ◽  
Author(s):  
Joachim Alexandre ◽  
René Rouet ◽  
Paolo-Emilio Puddu ◽  
Farzin Beygui ◽  
Alain Manrique ◽  
...  
Keyword(s):  
In Vitro Model ◽  
Border Zone ◽  
Premature Ventricular Contractions ◽  
Vitro Model

scholarly journals Effects of Myocardial Infarction on the Distribution and Transport of Nutrients and Oxygen in Porcine Myocardium

2012 ◽  
Vol 134 (10) ◽  
Author(s):  
Bryce H. Davis ◽  
Yoshihisa Morimoto ◽  
Chris Sample ◽  
Kevin Olbrich ◽  
Holly A. Leddy ◽  
...  
Keyword(s):  
Myocardial Infarction ◽  
Border Zone ◽  
Myoblast Differentiation ◽  
Vascular Density ◽  
Skeletal Myoblast ◽  
Infarcted Myocardium ◽  
Alkaline Phosphatase Staining ◽  
Infarct Border Zone ◽  
Infarct Border

One of the primary limitations of cell therapy for myocardial infarction is the low survival of transplanted cells, with a loss of up to 80% of cells within 3 days of delivery. The aims of this study were to investigate the distribution of nutrients and oxygen in infarcted myocardium and to quantify how macromolecular transport properties might affect cell survival. Transmural myocardial infarction was created by controlled cryoablation in pigs. At 30 days post-infarction, oxygen and metabolite levels were measured in the peripheral skeletal muscle, normal myocardium, the infarct border zone, and the infarct interior. The diffusion coefficients of fluorescein or FITC-labeled dextran (0.3–70 kD) were measured in these tissues using fluorescence recovery after photobleaching. The vascular density was measured via endogenous alkaline phosphatase staining. To examine the influence of these infarct conditions on cells therapeutically used in vivo, skeletal myoblast survival and differentiation were studied in vitro under the oxygen and glucose concentrations measured in the infarct tissue. Glucose and oxygen concentrations, along with vascular density were significantly reduced in infarct when compared to the uninjured myocardium and infarct border zone, although the degree of decrease differed. The diffusivity of molecules smaller than 40 kD was significantly higher in infarct center and border zone as compared to uninjured heart. Skeletal myoblast differentiation and survival were decreased stepwise from control to hypoxia, starvation, and ischemia conditions. Although oxygen, glucose, and vascular density were significantly reduced in infarcted myocardium, the rate of macromolecular diffusion was significantly increased, suggesting that diffusive transport may not be inhibited in infarct tissue, and thus the supply of nutrients to transplanted cells may be possible. in vitro studies mimicking infarct conditions suggest that increasing nutrients available to transplanted cells may significantly increase their ability to survive in infarct.

scholarly journals Angiocrine IGFBP3 Spatially Coordinates IGF Signaling During Neonatal Cardiac Regeneration

2021 ◽  
Author(s):  
Shah Ali ◽  
Waleed Elhelaly ◽  
Ngoc Uyen Nhi Nguyen ◽  
Shujuan Li ◽  
Ivan Menendez-Montes ◽  
...  
Keyword(s):  
Cardiac Injury ◽  
Cardiac Regeneration ◽  
Border Zone ◽  
Infarct Zone ◽  
Specific Expression ◽  
Spatial Release ◽  
Neonatal Injury ◽  
Infarct Border Zone ◽  
Infarct Border

To identify non cellautonomous effectors of cardiomyocyte mitosis, we analyzed a transcriptomic screen of regenerating and non regenerating neonatal hearts for differentially expressed secreted proteins, which we hypothesized could include candidate mitogens. We identified and validated IGFBP3, which has a Janus-like stabilizing and sequestering effect on IGF growth factors, as a neonatal injury associated secreted protein. IGFBP3 is expressed by and secreted from vascular cells in the neonatal heart after cardiac injury, notably in the infarct border zone. We found that global deletion of IGFBP3 blunted neonatal regeneration, while gain of function experiments using recombinant IGFBP3 and a transgenic mouse model uncovered a pro mitotic effect of IGFBP3 on cardiomyocytes in vitro and in the adult heart. We show that site specific expression of an IGFBP3 protease (PAPPA2) and its inhibitor (STC2) coordinate the spatial release of IGF2 in the infarct zone to regio selectively activate the INSR/ERK/AKT cell growth pathways in cardiomyocytes. Collectively, our work highlights the spatiotemporal orchestration of endothelial cardiomyocyte interactions that are required for neonatal cardiac regeneration.

An in vitro model for investigation of vitamin A effects on wound healing

2021 ◽  
pp. 1-6
Author(s):  
Hoda Keshmiri Neghab ◽  
Mohammad Hasan Soheilifar ◽  
Gholamreza Esmaeeli Djavid
Keyword(s):  
Wound Healing ◽  
Endothelial Cell ◽  
Vitamin A ◽  
In Vitro Model ◽  
Cellular Proliferation ◽  
Endothelial Cell Migration ◽  
Normal Fibroblast ◽  
Anti Inflammatory ◽  
Vitro Model

Abstract. Wound healing consists of a series of highly orderly overlapping processes characterized by hemostasis, inflammation, proliferation, and remodeling. Prolongation or interruption in each phase can lead to delayed wound healing or a non-healing chronic wound. Vitamin A is a crucial nutrient that is most beneficial for the health of the skin. The present study was undertaken to determine the effect of vitamin A on regeneration, angiogenesis, and inflammation characteristics in an in vitro model system during wound healing. For this purpose, mouse skin normal fibroblast (L929), human umbilical vein endothelial cell (HUVEC), and monocyte/macrophage-like cell line (RAW 264.7) were considered to evaluate proliferation, angiogenesis, and anti-inflammatory responses, respectively. Vitamin A (0.1–5 μM) increased cellular proliferation of L929 and HUVEC (p < 0.05). Similarly, it stimulated angiogenesis by promoting endothelial cell migration up to approximately 4 fold and interestingly tube formation up to 8.5 fold (p < 0.01). Furthermore, vitamin A treatment was shown to decrease the level of nitric oxide production in a dose-dependent effect (p < 0.05), exhibiting the anti-inflammatory property of vitamin A in accelerating wound healing. These results may reveal the therapeutic potential of vitamin A in diabetic wound healing by stimulating regeneration, angiogenesis, and anti-inflammation responses.

Sign in / Sign up

Export Citation Format

Share Document