scholarly journals Activity and Regulation of Na + -HCO 3 − Cotransporter in Immortalized Spontaneously Hypertensive Rat and Wistar–Kyoto Rat Proximal Tubular Epithelial Cells

Hypertension ◽  
2007 ◽  
Vol 49 (5) ◽  
pp. 1186-1193 ◽  
Author(s):  
Rui Pedrosa ◽  
Nuno Gonçalves ◽  
Ulrich Hopfer ◽  
Pedro A. Jose ◽  
Patrício Soares-da-Silva
Keyword(s):  
Epithelial Cells ◽  
Spontaneously Hypertensive Rat ◽  
Tubular Epithelial Cells ◽  
Spontaneously Hypertensive ◽  
Hypertensive Rat ◽  
Proximal Tubular Epithelial Cells ◽  
Proximal Tubular ◽  
Wistar Kyoto Rat ◽  
Wistar Kyoto

Hypothalamic angiotensin receptor subtypes in normotensive and hypertensive rats

1998 ◽  
Vol 275 (2) ◽  
pp. H703-H709 ◽  
Author(s):  
N. L. Han ◽  
M. K. Sim
Keyword(s):  
Angiotensin Ii ◽  
Spontaneously Hypertensive Rat ◽  
Renin Angiotensin System ◽  
Receptor Subtypes ◽  
Spontaneously Hypertensive ◽  
Hypertensive Rat ◽  
Angiotensin Iii ◽  
Displacement Experiments ◽  
Wistar Kyoto Rat ◽  
Wistar Kyoto

The binding of125I-labeled [Sar1,Ile8]angiotensin II to the hypothalamic membranes of the normotensive Wistar-Kyoto rat (WKY) and the spontaneously hypertensive rat (SHR) was studied. Displacement experiments with four centrally active angiotensins, losartan, and PD-123319 confirm the known existence of angiotensin AT1 and AT2 receptors in the rat hypothalamus. The values of the inhibitory constants for angiotensin II and PD-123319 in the SHR were significantly lower than the corresponding values in the WKY, indicating the possible existence of high-affinity hypothalamic AT1 and AT2 receptors for the two ligands in the SHR. The angiotensin AT1receptor was further separated into a 5′-guanylyl imidodiphosphate-sensitive and -nonsensitive subtype, indicating that one of the subtypes is G protein coupled. The SHR has significantly higher numbers of measurable AT1-receptor subtypes as well as AT2 receptor subtypes. The former data support the findings of other investigators showing that the hypothalamus of the SHR expressed more AT1A and AT1B mRNAs than that of the normotensive rat. Des-Asp1-angiotensin I, which is known to attenuate the central pressor action of angiotensin II and angiotensin III, acts on both the AT1 and AT2 receptors, although it has a higher affinity for the AT1receptors. The overall increase in the number of AT1 and AT2 receptors in the SHR is in line with the contention that the brain of the hypertensive rat, compared with that of the WKY, has a hyperactive renin-angiotensin system.

Effect of Sodium-Potassium-Dependent ATPase Inhibition on Noradrenaline-Activated Calcium Sensitivity of Mesenteric Resistance Vessels in Adult Spontaneously Hypertensive Rats

1980 ◽  
Vol 59 (s6) ◽  
pp. 203s-205s ◽  
Author(s):  
M. J. Mulvany ◽  
N. Nyborg ◽  
H. Nilsson
Keyword(s):  
Blood Vessels ◽  
Spontaneously Hypertensive Rat ◽  
Calcium Sensitivity ◽  
Sodium Potassium ◽  
Spontaneously Hypertensive ◽  
Rat Blood ◽  
Dependent Atpase ◽  
Hypertensive Rat ◽  
Wistar Kyoto Rat ◽  
Wistar Kyoto

1. We have investigated the noradrenaline-activated calcium sensitivity of 150 μm mesenteric resistance blood vessels from spontaneously hypertensive and control Wistar-Kyoto rats. 2. Under control conditions the spontaneously hypertensive rat blood vessels had a greater calcium sensitivity than the Wistar-Kyoto rat vessels. 3. In the presence of 1 mmol of ouabain/l, a treatment known to inhibit the sodium-potassium-dependent ATPase, the responses of the spontaneously hypertensive rat blood vessels were reduced more than those of the Wistar-Kyoto rat blood vessels, so that the responses of spontaneously hypertensive rat and Wistar-Kyoto rat blood vessels were then similar. 4. Similar results were obtained by removing external potassium, a procedure which should also inhibit the sodium-potassium-ATPase. 5. The results suggest that the greater noradrenaline-activated calcium sensitivity of spontaneously hypertensive rat blood vessels may be associated with an increased sodium-potassium-ATPase activity.

A morphological study of platelets from stroke-prone spontaneously hypertensive rat and Wistar Kyoto rat

1998 ◽  
Vol 5 (2) ◽  
pp. 119-124
Author(s):  
Masahiko Ikeda ◽  
James G White ◽  
Hideya Mizuno ◽  
Masaki Tabuchi ◽  
Takako Tomita
Keyword(s):  
Morphological Study ◽  
Spontaneously Hypertensive Rat ◽  
Spontaneously Hypertensive ◽  
Hypertensive Rat ◽  
Wistar Kyoto Rat ◽  
Wistar Kyoto

Defective D1-like receptor-mediated inhibition of the Cl−/HCO3− exchanger in immortalized SHR proximal tubular epithelial cells

2004 ◽  
Vol 286 (6) ◽  
pp. F1120-F1126 ◽  
Author(s):  
Rui Pedrosa ◽  
Pedro A. Jose ◽  
P. Soares-da-Silva
Keyword(s):  
Epithelial Cells ◽  
Apical Cell ◽  
Skf 38393 ◽  
Tubular Epithelial Cells ◽  
Receptor Stimulation ◽  
Anion Transporter ◽  
Proximal Tubular Epithelial Cells ◽  
Proximal Tubular ◽  
Wistar Kyoto ◽  
Stimulation Of

The sensitivity of the Cl−/HCO3− exchanger to dopamine D1- and D2-like receptor stimulation in immortalized renal proximal tubular epithelial cells from the spontaneous hypertensive rat (SHR) and Wistar-Kyoto rat (WKY) was examined. The activity of the Cl−/HCO3− exchanger (in pH U/s) in SHR cells (0.00191) was greater than in WKY cells (0.00126). The activity of Cl−/HCO3− exchanger was exclusively observed at the apical cell side and probably occurs through the SLC26A6 anion transporter that is expressed in both WKY and SHR cells. Stimulation of D1-like receptors with SKF-38393 markedly attenuated the HCO3−-dependent intracellular pH recovery in WKY cells but not in SHR cells. Stimulation of D2-like receptors with quinerolane did not alter Cl−/HCO3− exchanger activity in both WKY and SHR cells. The selective D1-like receptor antagonist SKF-83566 prevented the effect of SKF-38393. Both WKY and SHR cells responded to dibutyryl-cAMP (DBcAMP) with inhibition of the Cl−/HCO3− exchanger, and downregulation of PKA (overnight exposure to DBcAMP) abolished the inhibitory effect of both DBcAMP and SKF-38393 in WKY cells. Both SHR and WKY cells responded to forskolin with increases in the formation of cAMP. However, only WKY responded to SKF-38393 with increases in the formation of cAMP that was prevented by SKF-83566. It is concluded that WKY cells respond to D1-like dopamine receptor stimulation with inhibition of the apical Cl−/HCO3− (SLC26A6) exchanger and SHR cells have a defective D1-like dopamine response.

Sign in / Sign up

Export Citation Format

Share Document