scholarly journals Determination of Plasma Renin Concentration by Angiotensin I Immunoassay

1971 ◽  
Vol 28 (5_suppl_2) ◽  
Author(s):  
JAN R. STOCKIGT ◽  
R. DENNIS COLLINS ◽  
EDWARD G. BIGLIERI
Keyword(s):  
Plasma Renin ◽  
Angiotensin I ◽  
Renin Substrate ◽  
Plasma Renin Concentration ◽  
Postural Responses ◽  
Bilateral Adrenal Hyperplasia ◽  
Aldosterone Producing Adenoma ◽  
Desoxycorticosterone Acetate ◽  
Made In

Radioimmunoassay of angiotensin I was applied to the estimation of renin concentration after incubation of human plasma with an excess of renin substrate from plasma of anephric sheep. With this method markedly subnormal plasma renin levels were accurately measured. The technique was used in the study of 18 patients with proven aldosterone-producing adenoma (APA), 5 with hyperaldosteronism associated with bilateral adrenal hyperplasia, and 23 with essential hypertension. Plasma renin concentration (PRC) was extremely low in APA, and a significantly higher mean basal level of PRC was seen in hyperplastic aldosteronism ( P <0.01). Postural responses were seen in both groups, and PRC was least variable during recumbency. Comparison of basal recumbent PRC with urinary aldosterone after three days of desoxycorticosterone acetate (DOCA) accentuated the distinction between APA and hyperplasia. It is suggested that the prediction of a distinct APA can best be made in patients with hyperaldosteronism when the exact extent of renin suppression is taken into account and when DOCA is used to assess suppressibility of aldosterone. While such evaluation may defer surgery in some patients with correctable hypertension, it may reduce the number with persistent hypertension after bilateral adrenalectomy.

RENIN LEVELS IN THE KANGAROO, THE WOMBAT AND OTHER MARSUPIAL SPECIES

1971 ◽  
Vol 51 (1) ◽  
pp. 79-90 ◽  
Author(s):  
PAMELA A. SIMPSON ◽  
J. R. BLAIR-WEST
Keyword(s):  
Renin Angiotensin System ◽  
Plasma Renin ◽  
Angiotensin I ◽  
Renin Substrate ◽  
Angiotensin System ◽  
Plasma Renin Concentration ◽  
Red Kangaroo ◽  
Grey Kangaroo ◽  
Common Wombat ◽  
Marsupial Species

SUMMARY A renin—angiotensin system was shown to be present in several marsupial species in plasma and homogenates of the renal cortex. Species studied were: Eastern Grey kangaroo (Macropus giganteus), Red kangaroo (Megaleia rufa), common wombat (Vombatus hirsutus), pademelon (Thylogale billardierii), Bennett's wallaby (Wallabia rufogrisea frutica), a quokka (Setonix brachyurus) and a tiger cat (Dasyurus maculatus). Renin-substrate was found in the plasma of the Eastern Grey kangaroo, the Red kangaroo and the wombat. Renin was shown to be present in the plasma of all species by incubation alone or with heterologous marsupial renin-substrate. Plasma renin concentration and renal renin content were estimated by an established method using standard sheep renin-substrate. Plasma renin concentration was high, suggesting that marsupial renins have a high affinity for sheep substrate; renal renin estimates were low relative to eutherian species, suggesting that renal storage may be small. Plasma renin concentration and renal renin levels were proportionately related. Renin levels were consistently lowest in the wombat. Bilateral nephrectomy of an Eastern Grey kangaroo reduced plasma renin concentration to zero and increased renin-substrate concentration eightfold. The angiotensin-like incubation product from Eastern Grey kangaroo renin-substrate did not cross react with antibodies to [5-Ile]-angiotensin I, suggesting that the product has a different sequence of amino acids.

Determination of Plasma Renin Concentration by Angiotensin I Immunoassay

1971 ◽  
Vol 28 (5 Suppl 2) ◽  
pp. II-175-II-191 ◽  
Author(s):  
JAN R. STOCKIGT ◽  
R. DENNIS COLLINS ◽  
EDWARD G. BIGLIERI
Keyword(s):  
Plasma Renin ◽  
Angiotensin I ◽  
Plasma Renin Concentration

Measurement of inactive renin in normal, nephrectomized, and adrenalectomized rats

1991 ◽  
Vol 69 (9) ◽  
pp. 1381-1384 ◽  
Author(s):  
Knud Poulsen ◽  
Arne Høj Nielsen ◽  
Arne Johannessen
Keyword(s):  
Animal Model ◽  
Exchange Resin ◽  
Cation Exchange Resin ◽  
Plasma Renin ◽  
Rat Plasma ◽  
Angiotensin I ◽  
Renin Substrate ◽  
Suitable Animal Model ◽  
Inactive Renin ◽  
Adrenalectomized Rats

In a new method for measurement of inactive rat plasma renin, the trypsin generated angiotensin I immunoreactive material, which was HPLC characterized as similar to tetradecapeptide renin substrate, is removed by a cation exchange resin before the renin incubation step. The method also corrects for trypsin destruction of endogenous angiotensinogen by the addition of exogenous angiotensinogen. When measured with this method inactive renin in rat plasma decreased after nephrectomy and increased after adrenalectomy. This is in accordance with findings in humans. A sexual dimorphism of prorenin (inactive renin) in rat plasma, similar to that reported in humans and mice, was demonstrated. Thus, inactive renin in the rat is no exception among species, and the rat might be a suitable animal model for further studies dealing with the physiology of prorenin in plasma and tissues.Key words: angiotensinogen, inactive renin, renin.

SERIAL ESTIMATION OF PLASMA RENIN CONCENTRATION DURING PREGNANCY AND AFTER PARTURITION

1966 ◽  
Vol 35 (4) ◽  
pp. 373-378 ◽  
Author(s):  
J. J. BROWN ◽  
D. L. DAVIES ◽  
P. B. DOAK ◽  
A. F. LEVER ◽  
J. I. S. ROBERTSON
Keyword(s):  
Pregnant Women ◽  
Plasma Renin ◽  
Plasma Renin Concentration ◽  
Before And After ◽  
Normal Women ◽  
Made In

SUMMARY Plasma renin concentration has been measured in normal women at intervals throughout pregnancy. Further measurements have been made in the days and hours before and after delivery of the foetus and placenta. Plasma renin was consistently raised in the majority of pregnant women and did not change markedly until 24 hr. or more after delivery. The significance of these findings is discussed.

ESTIMATION OF MARSUPIAL RENIN USING MARSUPIAL RENIN-SUBSTRATE

1972 ◽  
Vol 53 (1) ◽  
pp. 125-130 ◽  
Author(s):  
PAMELA A. SIMPSON ◽  
J. R. BLAIR-WEST
Keyword(s):  
Substrate Concentration ◽  
Structural Difference ◽  
Plasma Renin ◽  
Angiotensin I ◽  
Ph Optimum ◽  
Ph Profile ◽  
Renin Substrate ◽  
Grey Kangaroo ◽  
Rate Of Reaction ◽  
Highly Correlated

SUMMARY Bilateral nephrectomy of an Eastern Grey kangaroo (Macropus giganteus) increased plasma renin-substrate concentration approximately tenfold when compared with intact kangaroos. A preparation made from this plasma had a renin-substrate concentration of 3000 ng/ml. A pH profile of rate of reaction with pig renin had an optimum at pH 5·39. By comparison, the pH optimum of sheep renin-substrate was pH 6·15. Estimates of plasma renin concentration for kangaroos, wombats and wallabies, using kangaroo renin-substrate or sheep renin-substrate were highly correlated. Results from incubation with sheep renin-substrate were greater and hence indicate the advantage in using this substrate for marsupial renin estimation. The consistently large difference between sheep and kangaroo renin-substrate when incubated with renin from marsupial and eutherian species appears to be due to a structural difference between the two substrates, probably near the C-terminal end of the angiotensin I molecule.

Methods for serial study of renin-angiotensin system in the unanesthetized rat

1975 ◽  
Vol 228 (2) ◽  
pp. 369-375 ◽  
Author(s):  
JS Carvalho ◽  
R Shapiro ◽  
P Hopper ◽  
LB Page
Keyword(s):  
Renin Angiotensin System ◽  
Plasma Renin ◽  
Rat Plasma ◽  
Angiotensin I ◽  
Ether Anesthesia ◽  
Renin Substrate ◽  
Angiotensin System ◽  
Arterial Blood ◽  
Sympathetic Nervous ◽  
Improved Technique

Micromethods for measurement of plasma renin concentration (PRC) and plasma renin-substrate concentration (PSC) have been developed for rat plasma with radioimmunoassay of angiotensin I. An improved technique for aortic implantation of plastic cannulas was developed for use in experiments 1-2 wk in duration. The effects on components of renin system of anesthesia and tail cutting were studied. Arterial blood was sampled through cannulas without animal manipulation. PRC varied little in unanesthetized rats, was moderately and variably increased during pentobarbital anesthesia, and was markedly and consistently elevated during ether anesthesia. PSC was unchanged during anesthesia. PRC was increased in blood obtained by tail cutting within 1-2 min after cutting. With the use of the methods and techniques described here serial studies of the renin system in plasma of unanesthetized rats are shown to be feasible. A role for the sympathetic nervous system in the mediation of renin secretion by ether is proposed.

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