Isolation and characterization of new microsatellite markers for application in population genetic studies of Caiman latirostris and related species

2015 ◽  
Vol 36 (2) ◽  
pp. 175-180 ◽  
Author(s):  
Patricia Susana Amavet ◽  
Eva Carolina Rueda ◽  
Pablo Ariel Siroski ◽  
Alejandro Larriera ◽  
Beatriz Ofelia Saidman
Keyword(s):  
Related Species ◽  
Mating Systems ◽  
Sustainable Use ◽  
Bioinformatics Tool ◽  
Genetic Studies ◽  
Shared Data ◽  
Isolation And Characterization ◽  
Generation Sequencing ◽  
Systems Studies

Wild populations ofCaiman latirostrisare subject to sustainable use programs in Argentina, becoming a species with important impact in the regional economy, based in their skin and meat. Genetic studies are fundamental to acquire information on important parameters for conservation and management, which may be obtained from analysis of molecular markers. Some microsatellites have been previously isolated in this species, but due to some difficulties in using them, we obtained new ones using Next Generation Sequencing approach. This study reports eight new microsatellites forC. latirostrisand tests their utility in a related species,Caiman yacare, with successful application in population genetics and mating systems studies. In addition, we shared data about a novel and fast bioinformatics tool to find microsatellites and to design their corresponding primers.

Isolation and characterization of microsatellites markers for two South American frogs (Leptodactylus bufonius and L. chaquensis) using next generation sequencing

2014 ◽  
Vol 35 (4) ◽  
pp. 405-412 ◽  
Author(s):  
Gabriel P. Faggioni ◽  
Gabriel P. Faggioni ◽  
Kelly R. Zamudio ◽  
Gabriel P. Faggioni ◽  
Kelly R. Zamudio ◽  
...  
Keyword(s):  
Fragmented Landscape ◽  
South American ◽  
Genetic Studies ◽  
Forestry Practices ◽  
Isolation And Characterization ◽  
Conservation Genetic ◽  
Local Species ◽  
Threatened Habitat ◽  
Generation Sequencing

Leptodactylus bufonius (Vizcacheras’ white-lipped frog) and L. chaquensis (Cei’s white-lipped frog) are pond-breeding frogs that inhabit the Chaco and surrounding savanna-like formations in South America. Throughout the Chacoan plain, the combined impacts of livestock and forestry practices have led to a highly fragmented landscape and an impoverished ecological system, threatening local species. We cloned and characterized new microsatellite markers for both species. These markers will be useful for behavioral and conservation genetic studies of populations throughout this threatened habitat.

Isolation and Characterization of LMW-GS Glu-A3 in Common Wheat Related Species

2017 ◽  
Vol 43 (6) ◽  
pp. 829
Author(s):  
Xue DONG ◽  
Meng LIU ◽  
Xian-Lin ZHAO ◽  
Yu-Mei FENG ◽  
Yan YANG
Keyword(s):  
Common Wheat ◽  
Related Species ◽  
Isolation And Characterization

scholarly journals Characterization of Unknown Orthobunya-Like Viruses from India

Viruses ◽  
2018 ◽  
Vol 10 (9) ◽  
pp. 451 ◽  
Author(s):  
Shannon Whitmer ◽  
Pragya Yadav ◽  
Prasad Sarkale ◽  
Gouri Chaubal ◽  
Alicia Francis ◽  
...  
Keyword(s):  
Next Generation Sequencing ◽  
Febrile Illness ◽  
Geographic Region ◽  
Emerging Viruses ◽  
Simbu Serogroup ◽  
Isolation And Characterization ◽  
Karnataka State ◽  
Next Generation Sequencing Ngs ◽  
Generation Sequencing

Next-generation sequencing (NGS) of agents causing idiopathic human diseases has been crucial in the identification of novel viruses. This study describes the isolation and characterization of two novel orthobunyaviruses obtained from a jungle myna and a paddy bird from Karnataka State, India. Using an NGS approach, these isolates were classified as Cat Que and Balagodu viruses belonging to the Manzanilla clade of the Simbu serogroup. Closely related viruses in the Manzanilla clade have been isolated from mosquitos, humans, birds, and pigs across a wide geographic region. Since Orthobunyaviruses exhibit high reassortment frequency and can cause acute, self-limiting febrile illness, these data suggest that human and livestock infections of the Oya/Cat Que/Manzanilla virus may be more widespread and/or under-reported than anticipated. It therefore becomes imperative to identify novel and unknown viruses in order to understand their role in human and animal pathogenesis. The current study is a step forward in this regard and would act as a prototype method for isolation, identification and detection of several other emerging viruses.

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