scholarly journals Genetically Encoded Calcium Indicators for In Situ Functional Studies of Corneal Nerves

2020 ◽  
Vol 61 (13) ◽  
pp. 10
Author(s):  
Matthew T. McPheeters ◽  
Brecken J. Blackburn ◽  
William J. Dupps ◽  
Andrew M. Rollins ◽  
Michael W. Jenkins
Keyword(s):  
Functional Studies ◽  
Calcium Indicators ◽  
Corneal Nerves ◽  
Genetically Encoded Calcium Indicators

Imaging of the corneal nerves ex vivo and in vivo using genetically-encoded calcium indicators

2021 ◽  
Author(s):  
Matthew T. McPheeters ◽  
Brecken J. Blackburn ◽  
William J. Dupps ◽  
Andrew M. Rollins ◽  
Michael W. Jenkins
Keyword(s):  
Ex Vivo ◽  
Calcium Indicators ◽  
Corneal Nerves ◽  
Genetically Encoded Calcium Indicators

In situ x-ray photoelectron spectroscopic and density-functional studies of Si atoms adsorbed on a C[sub 60] film

2004 ◽  
Vol 121 (22) ◽  
pp. 11351 ◽  
Author(s):  
Jun Onoe ◽  
Aiko Nakao ◽  
Toshiki Hara
Keyword(s):  
Density Functional ◽  
X Ray ◽  
Functional Studies

Genetically Encoded Calcium Indicators

2008 ◽  
Vol 108 (5) ◽  
pp. 1550-1564 ◽  
Author(s):  
Marco Mank ◽  
Oliver Griesbeck
Keyword(s):  
Calcium Indicators ◽  
Genetically Encoded Calcium Indicators

scholarly journals Reverse transcriptase sequences from mulberry LTR retrotransposons: characterization analysis

2017 ◽  
Vol 12 (1) ◽  
pp. 266-276
Author(s):  
Bi Ma ◽  
Lulu Kuang ◽  
Youchao Xin ◽  
Fei Hou ◽  
Ningjia He
Keyword(s):  
Reverse Transcriptase ◽  
Evolutionary Dynamics ◽  
Selective Pressure ◽  
Sequence Divergence ◽  
Degenerate Primers ◽  
Random Mutation ◽  
Functional Studies ◽  
Study Support ◽  
Element Transfer

AbstractCopia and Gypsy play important roles in structural, functional and evolutionary dynamics of plant genomes. In this study, a total of 106 and 101, Copia and Gypsy reverse transcriptase (rt) were amplified respectively in the Morus notabilis genome using degenerate primers. All sequences exhibited high levels of heterogeneity, were rich in AT and possessed higher sequence divergence of Copia rt in comparison to Gypsy rt. Two reasons are likely to account for this phenomenon: a) these elements often experience deletions or fragmentation by illegitimate or unequal homologous recombination in the transposition process; b) strong purifying selective pressure drives the evolution of these elements through “selective silencing” with random mutation and eventual deletion from the host genome. Interestingly, mulberry rt clustered with other rt from distantly related taxa according to the phylogenetic analysis. This phenomenon did not result from horizontal transposable element transfer. Results obtained from fluorescence in situ hybridization revealed that most of the hybridization signals were preferentially concentrated in pericentromeric and distal regions of chromosomes, and these elements may play important roles in the regions in which they are found. Results of this study support the continued pursuit of further functional studies of Copia and Gypsy in the mulberry genome.

Labeling Microglia with Genetically Encoded Calcium Indicators

2019 ◽  
pp. 243-265 ◽  
Author(s):  
Yajie Liang ◽  
Olga Garaschuk
Keyword(s):  
Calcium Indicators ◽  
Genetically Encoded Calcium Indicators

scholarly journals Genetically Encoded Calcium Indicators: A New Tool in Renal Hypertension Research

2019 ◽  
Vol 6 ◽  
Author(s):  
Cheng Zhong ◽  
Johanna Schleifenbaum
Keyword(s):  
Renal Hypertension ◽  
Calcium Indicators ◽  
Genetically Encoded Calcium Indicators

scholarly journals Expression of the yes proto-oncogene in cerebellar Purkinje cells.

1989 ◽  
Vol 9 (10) ◽  
pp. 4545-4549 ◽  
Author(s):  
M Sudol ◽  
C F Kuo ◽  
L Shigemitsu ◽  
A Alvarez-Buylla
Keyword(s):  
In Situ Hybridization ◽  
Purkinje Cell ◽  
Gene Product ◽  
Purkinje Cells ◽  
Immunofluorescent Staining ◽  
Cell Degeneration ◽  
Functional Studies ◽  
Cerebellar Purkinje Cells ◽  
The Brain

To identify the kinds of cells in the brain that express the yes proto-oncogene, we examined chicken brains by using immunofluorescent staining and in situ hybridization. Both approaches showed that the highest level of the yes gene product was in cerebellar Purkinje cells. In addition, we analyzed Purkinje cell degeneration (pcd) mutant mice. The level of yes mRNA in cerebella of pcd mutants was four times lower than that found in cerebella of normal littermates. Our studies point to Purkinje cells as an attractive model for functional studies of the yes protein.

Functional studies on in situ-like mitochondria isolated in the presence of polyvinyl pyrrolidone

1985 ◽  
Vol 806 (1) ◽  
pp. 1-8 ◽  
Author(s):  
Klaus Wrogemann ◽  
Edward G. Nylen ◽  
Ian Adamson ◽  
Shri V. Pande
Keyword(s):  
Polyvinyl Pyrrolidone ◽  
Functional Studies

Flounder (Paralichthys olivaceus) FoxD1 and its regulation on the expression of myogenic regulatory factor, MyoD

Biologia ◽  
2012 ◽  
Vol 67 (5) ◽  
Author(s):  
Yuqing Zhang ◽  
Xungang Tan ◽  
Wei Sun ◽  
Pei-Jun Zhang
Keyword(s):  
Muscle Development ◽  
Paralichthys Olivaceus ◽  
Fish Muscle ◽  
Regulatory Factor ◽  
Myogenic Regulatory Factor ◽  
Functional Studies ◽  
And Function ◽  
Myod Expression ◽  
Adaxial Cells

AbstractIt has been reported that FoxD1 plays important roles in formation of several different tissues, such as retina and kidney in vertebrates. The function of FoxD1 in muscle development is, however, unclear although it is expressed in muscle cells in zebrafish. Muscles are the major tissue in fish, which serves as a rich protein source in our diet. To further understand the function of FoxD1 in fish muscle development, here we isolated and characterized the FoxD1 gene from flounder (Paralichthys olivaceus), a valuable sea food and an important fish species in aquaculture in Asia. We analyzed its expression pattern and function in regulating myogenic regulatory factor, MyoD, one of the earliest marker of myogenic commitment. In situ hybridization revealed that FoxD1 was expressed in the tailbud, adaxial cells, posterior intestine, forebrain, midbrain and half of the retina in flounder embryos. Functional studies demonstrated that when flounder FoxD1 was over-expressed in zebrafish by microinjection, MyoD expression was decreased, suggesting that FoxD1 may be involved in myogenesis by regulating the expression of MyoD.

Sign in / Sign up

Export Citation Format

Share Document