Skin Flap Tension and Wound Slough: Correlation with Laser Doppler Velocimetry

1982 ◽  
Vol 90 (2) ◽  
pp. 185-187 ◽  
Author(s):  
Wayne F. Larrabee ◽  
G. Allen Holloway ◽  
Ron E. Trachy ◽  
Dwight Sutton
Keyword(s):  
Blood Flow ◽  
Laser Doppler Velocimetry ◽  
Skin Flap ◽  
Quantitative Measure ◽  
Laser Doppler ◽  
Flap Necrosis ◽  
Skin Tension ◽  
Flap Viability ◽  
Recent Origin ◽  
Cutaneous Blood

The effects of wound tension on flap viability and blood flow are investigated in an animal model using the domestic piglet. In flaps with an impaired blood supply, wound closing tensions >250 g resulted in a significantly increased incidence of flap necrosis. The laser Doppler is a device of recent origin for the measurement of cutaneous blood flow. In this study, it provided a simple quantitative measure of the inverse relationship between skin tension and blood flow.

Role of Growth Factors in Improved Skin Flap Viability Caused by Phosphodiesterase-5 Inhibitor

2010 ◽  
Vol 76 (6) ◽  
pp. 614-617 ◽  
Author(s):  
Bradley C. Bandera ◽  
Thach Pham ◽  
Crystal Hill-Pryor ◽  
Mariama Bah-Sow ◽  
Nelson Franco ◽  
...  
Keyword(s):  
Blood Flow ◽  
Growth Factors ◽  
Growth Factor ◽  
Ribonucleic Acid ◽  
Skin Flap ◽  
Flap Necrosis ◽  
Angiogenic Growth Factors ◽  
Affected Area ◽  
Flap Viability ◽  
Phosphodiesterase 5

Flap necrosis is one of the major complications of reconstructive surgery and sildenafil citrate has been shown to decrease flap necrosis in preclinical animal models. However, the mechanisms underlying sildenafil's therapeutic efficacy are not known. As with other phosphodiesterase 5 selective inhibitors, sildenafil causes vasodilation and enhanced blood flow. In addition, sildenafil can also alter gene expression. This study is designed to test the hypothesis that increased expression of angiogenic growth factors may be responsible for therapeutic efficacy of sildenafil. A modified McFarlane flap measuring 3 X 10 cm was created on the dorsal skin of male Sprague-Dawley rats. For growth factor expression experiment, rats were administered either vehicle or sildenafil 10 mg/Kg intraperitoneal (IP). Ribonucleic acid (RNA) extracted from skin flap was analyzed to assess the messenger ribonucleic acid (mRNA) levels of different angiogenic growth factors. For skin flap viability experiment, fibrin film impregnated with vehicle, fibroblast growth factor (FGF) (5.0 μg) or vascular endothelial growth factor (VEGF) (2.0 μg) was applied to the wound. The skin flap was then returned to its native position and stapled in place. Total affected area (area of necrosis and blood flow stasis) of each rat on postoperative day 14 was analyzed with orthogonal polarization spectral imaging. Daily systemic treatment with sildenafil significantly ( P < 0.05) increased the expression of FGF1 and FGF Receptor 3 on postoperative day 3 by 5.08- and 4.78-fold, respectively. In addition, sildenafil increased the expression of VEGF-A, VEGF-B, and VEGF-C by 2.66-, 2.02-, and 2.00-fold, respectively. Subcutaneous treatment with FGF but not VEGF-A tended to decrease total affected area in rats. These data demonstrate that sildenafil altered the expression of FGF and VEGF. Altered expression of growth factors may be, at least partly, responsible for the beneficial effects of sildenafil citrate on skin viability.

Laser Doppler velocimetry: a new technique for the measurement of intestinal mucosal blood flow

1984 ◽  
Vol 30 (4) ◽  
pp. 225-230 ◽  
Author(s):  
Andrew D. Feld ◽  
Joseph D. Fondacaro ◽  
G. Allen Holloway ◽  
Eugene D. Jacobson
Keyword(s):  
Blood Flow ◽  
Laser Doppler Velocimetry ◽  
Mucosal Blood Flow ◽  
Laser Doppler ◽  
New Technique ◽  
Doppler Velocimetry ◽  
A New Technique

Hybrid blood flow probe for simultaneous H2 clearance and laser-Doppler velocimetry

1987 ◽  
Vol 253 (4) ◽  
pp. G573-G581 ◽  
Author(s):  
G. R. DiResta ◽  
J. W. Kiel ◽  
G. L. Riedel ◽  
P. Kaplan ◽  
A. P. Shepherd
Keyword(s):  
Blood Flow ◽  
Laser Doppler Velocimetry ◽  
Regional Blood Flow ◽  
Laser Doppler ◽  
Doppler Velocimetry ◽  
Flow Probe ◽  
Flow Measurements ◽  
Blood Flow Measurements ◽  
Light Guides ◽  
The Relationship

To perform two independent regional blood flow measurements in tissue volumes of similar dimensions, we designed a hybrid blood flow probe capable of measuring regional perfusion by both laser-Doppler velocimetry (LDV) and H2 clearance. The probe consisted of two fiber-optic light guides to conduct light between the surface of tissue of interest and a laser-Doppler blood flowmeter. Also contained within the probe were a platinum 25-microns H2-sensing electrode and a 125-microns H2-generating electrode. The probe can thus be used to measure local perfusion with H2 clearance. The H2 can either be inhaled or can be generated electrochemically at the locus of interest. Evaluation of the probe in the canine gastric mucosa indicated 1) that the relationship between mucosal flow measurements made simultaneously with H2 clearance and LDV was highly significant and linear and 2) that H2 clearance could potentially be used to calibrate the laser-Doppler blood flowmeter in absolute units. The methods of constructing the flow probes are discussed in detail.

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