Laser Doppler velocimetry: a new technique for the measurement of intestinal mucosal blood flow

1984 ◽  
Vol 30 (4) ◽  
pp. 225-230 ◽  
Author(s):  
Andrew D. Feld ◽  
Joseph D. Fondacaro ◽  
G. Allen Holloway ◽  
Eugene D. Jacobson
Keyword(s):  
Blood Flow ◽  
Laser Doppler Velocimetry ◽  
Mucosal Blood Flow ◽  
Laser Doppler ◽  
New Technique ◽  
Doppler Velocimetry ◽  
A New Technique

Gastric mucosal blood flow measured by laser-Doppler velocimetry

1985 ◽  
Vol 249 (4) ◽  
pp. G539-G545 ◽  
Author(s):  
J. W. Kiel ◽  
G. L. Riedel ◽  
G. R. DiResta ◽  
A. P. Shepherd
Keyword(s):  
Blood Flow ◽  
Laser Doppler Velocimetry ◽  
Reactive Hyperemia ◽  
Arterial Occlusion ◽  
Mucosal Blood Flow ◽  
Gastric Mucosal Blood Flow ◽  
Laser Doppler ◽  
Doppler Velocimetry ◽  
Blood Flows ◽  
Ldv Measurement

To determine the feasibility of measuring gastric mucosal blood flow by laser-Doppler velocimetry (LDV), we utilized two LDV flowmeters to monitor blood flow in mucosa and serosa of chambered canine stomach. In isolated, nonautoregulating gastric segments vasodilated with isoproterenol, LDV mucosal and muscularis blood flows were both linearly related to total electromagnetic blood flow during step increases in perfusion pressure. To assess the depth of the LDV measurement, we recorded reactive hyperemia following arterial occlusion. Reactive hyperemia was frequently registered in the mucosa but rarely in muscularis. Placing a layer of nonperfused mucosa-submucosa between the probe and the perfused mucosa abolished the resting LDV mucosal flow signal and attenuated the recording of peak hyperemia by 85%. Furthermore, intra-arterial infusions of both adenosine and isoproterenol frequently increased LDV mucosal flow and decreased LDV muscularis flow, although total flow was consistently increased. These findings indicate that our LDV instruments yield linear, superficial measurements of gastric blood flow in either mucosa or muscularis. Although calibration in absolute units remains to be achieved, our results demonstrate that LDV is a practical means of studying the gastric mucosal microcirculation.

Measurement of mucosal blood flow in the canine intestine with laser doppler velocimetry

Life Sciences ◽  
1982 ◽  
Vol 31 (14) ◽  
pp. 1509-1517 ◽  
Author(s):  
Andrew D. Feld ◽  
Joseph D. Fondacaro ◽  
G.Allen Holloway ◽  
Eugene D. Jacobson
Keyword(s):  
Blood Flow ◽  
Laser Doppler Velocimetry ◽  
Mucosal Blood Flow ◽  
Laser Doppler ◽  
Doppler Velocimetry

Hybrid blood flow probe for simultaneous H2 clearance and laser-Doppler velocimetry

1987 ◽  
Vol 253 (4) ◽  
pp. G573-G581 ◽  
Author(s):  
G. R. DiResta ◽  
J. W. Kiel ◽  
G. L. Riedel ◽  
P. Kaplan ◽  
A. P. Shepherd
Keyword(s):  
Blood Flow ◽  
Laser Doppler Velocimetry ◽  
Regional Blood Flow ◽  
Laser Doppler ◽  
Doppler Velocimetry ◽  
Flow Probe ◽  
Flow Measurements ◽  
Blood Flow Measurements ◽  
Light Guides ◽  
The Relationship

To perform two independent regional blood flow measurements in tissue volumes of similar dimensions, we designed a hybrid blood flow probe capable of measuring regional perfusion by both laser-Doppler velocimetry (LDV) and H2 clearance. The probe consisted of two fiber-optic light guides to conduct light between the surface of tissue of interest and a laser-Doppler blood flowmeter. Also contained within the probe were a platinum 25-microns H2-sensing electrode and a 125-microns H2-generating electrode. The probe can thus be used to measure local perfusion with H2 clearance. The H2 can either be inhaled or can be generated electrochemically at the locus of interest. Evaluation of the probe in the canine gastric mucosa indicated 1) that the relationship between mucosal flow measurements made simultaneously with H2 clearance and LDV was highly significant and linear and 2) that H2 clearance could potentially be used to calibrate the laser-Doppler blood flowmeter in absolute units. The methods of constructing the flow probes are discussed in detail.

Differences in reactive hyperemia between the intestinal mucosa and muscularis

1984 ◽  
Vol 247 (6) ◽  
pp. G617-G622
Author(s):  
A. P. Shepherd ◽  
G. L. Riedel
Keyword(s):  
Blood Flow ◽  
Metabolic Rate ◽  
Laser Doppler Velocimetry ◽  
Reactive Hyperemia ◽  
Arterial Occlusion ◽  
Oxygen Demand ◽  
Laser Doppler ◽  
Doppler Velocimetry ◽  
Total Blood ◽  
Total Blood Flow

In a previous study of regional intestinal blood flow by laser-Doppler velocimetry, we noted that the mucosa displayed reactive hyperemia following arterial occlusion but that the muscularis did not. Therefore, to determine whether this observation is generally valid, we compared responses of the mucosa and muscularis externa to arterial occlusion. We measured total blood flow to isolated loops of canine small bowel with an electromagnetic flow probe on the supply artery; blood flow either in the mucosa or in the muscularis was measured by laser-Doppler velocimetry. Mucosal and total blood flow consistently showed reactive hyperemia in response to a 60-s occlusion, but the muscularis did not. To determine whether metabolic rate influenced reactive hyperemia, we increased enteric oxygen uptake by placing 5% bile and transportable solutes in the lumen; these agents increased oxygen consumption by 36%. After a 60-s occlusion, the durations of both total and mucosal reactive hyperemia were significantly prolonged by increased metabolic rate. Similarly, the payback-to-debt ratios in both total and mucosal blood flows were significantly increased at elevated metabolic rate. These data support the conclusions that reactive hyperemia occurs more frequently and has a greater magnitude in the mucosa compared with the muscularis and both total and mucosal reactive hyperemia are strongly influenced by the preocclusive oxygen demand. These findings therefore constitute further evidence that metabolic factors contribute to reactive hyperemia in the intestinal circulation.

Blood flow velocity imaging of malignant melanoma by micro multipoint laser Doppler velocimetry

2010 ◽  
Vol 97 (10) ◽  
pp. 103702 ◽  
Author(s):  
H. Ishida ◽  
T. Andoh ◽  
S. Akiguchi ◽  
H. Shirakawa ◽  
D. Kobayashi ◽  
...  
Keyword(s):  
Blood Flow ◽  
Malignant Melanoma ◽  
Flow Velocity ◽  
Blood Flow Velocity ◽  
Laser Doppler Velocimetry ◽  
Laser Doppler ◽  
Doppler Velocimetry ◽  
Velocity Imaging
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