Comparative study of optical coherence tomography angiography algorithms for rodent retinal imaging

2021 ◽  
pp. 153537022110245
Author(s):  
Arash Dadkhah ◽  
Dhruba Paudel ◽  
Shuliang Jiao
Keyword(s):  
Optical Coherence Tomography ◽  
Optical Coherence Tomography Angiography ◽  
Retinal Imaging ◽  
Biological Tissues ◽  
Vessel Diameter ◽  
Optical Coherence ◽  
Speckle Contrast ◽  
Phase Variance ◽  
Optical Microangiography

Optical coherence tomography angiography (OCTA) is a functional extension of optical coherence tomography for non-invasive in vivo three-dimensional imaging of the microvasculature of biological tissues. Several algorithms have been developed to construct OCTA images from the measured optical coherence tomography signals. In this study, we compared the performance of three OCTA algorithms that are based on the variance of phase, amplitude, and the complex representations of the optical coherence tomography signals for rodent retinal imaging, namely the phase variance, improved speckle contrast, and optical microangiography. The performance of the different algorithms was evaluated by comparing the quality of the OCTA images regarding how well the vasculature network can be resolved. Quantities that are widely used in ophthalmic studies including blood vessel density, vessel diameter index, vessel perimeter index, vessel complexity index were also compared. Results showed that both the improved speckle contrast and optical microangiography algorithms are more robust than phase variance, and they can reveal similar vasculature features while there are statistical differences in the calculated quantities.

scholarly journals Evaluating Retinal and Choroidal Perfusion Changes after Isometric and Dynamic Activity Using Optical Coherence Tomography Angiography

Diagnostics ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 808
Author(s):  
Max Philipp Brinkmann ◽  
Nikolas Xavier Kibele ◽  
Michelle Prasuhn ◽  
Vinodh Kakkassery ◽  
Mario Damiano Toro ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
Optical Coherence Tomography Angiography ◽  
Isometric Exercise ◽  
Dynamic Exercise ◽  
Exercise Heart Rate ◽  
Optical Coherence ◽  
Dynamic Activity ◽  
Capillary Plexus ◽  
Choroidal Perfusion

Optical coherence tomography angiography (OCTA) is a non-invasive tool for imaging and quantifying the retinal and choroidal perfusion state in vivo. This study aimed to evaluate the acute effects of isometric and dynamic exercise on retinal and choroidal sublayer perfusion using OCTA. A pilot study was conducted on young, healthy participants, each of whom performed a specific isometric exercise on the first day and a dynamic exercise the day after. At baseline and immediately after the exercise, heart rate (HR), mean arterial pressure (MAP), superficial capillary plexus perfusion (SCPP), deep capillary plexus perfusion (DCPP), choriocapillaris perfusion (CCP), Sattlers’s layer perfusion (SLP), and Haller’s layer perfusion (HLP) were recorded. A total of 34 eyes of 34 subjects with a mean age of 32.35 ± 7.87 years were included. HR as well as MAP increased significantly after both types of exercise. Both SCPP and DCPP did not show any significant alteration due to isometric or dynamic exercise. After performing dynamic exercise, CCP, SLP, as well as HLP significantly increased. Changes in MAP correlated significantly with changes in HLP after the dynamic activity. OCTA-based analysis in healthy adults following physical activity demonstrated a constant retinal perfusion, supporting the theory of autoregulatory mechanisms. Dynamic exercise, as opposed to isometric activity, significantly changed choroidal perfusion. OCTA imaging may represent a novel and sensitive tool to expand the diagnostic spectrum in the field of sports medicine.

Spatio-temporal optical coherence tomography STOC-T for in-vivo retinal imaging

2021 ◽  
Author(s):  
Egidijus Auksorius ◽  
Piotr Wegrzyn ◽  
Ieva Zickiene ◽  
Slawomir Tomczewski ◽  
Karolis Adomavicius ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
Retinal Imaging ◽  
Optical Coherence ◽  
Spatio Temporal

scholarly journals A practical guide to optical coherence tomography angiography interpretation

2020 ◽  
Vol 6 (1) ◽  
Author(s):  
Eugenia Custo Greig ◽  
Jay S. Duker ◽  
Nadia K. Waheed
Keyword(s):  
Optical Coherence Tomography ◽  
Optical Coherence Tomography Angiography ◽  
Image Interpretation ◽  
Age Related Macular Degeneration ◽  
Optical Coherence ◽  
Clinical Practices ◽  
Imaging Device ◽  
Age Related ◽  
Retinal Microvasculature

Abstract Background Optical coherence tomography angiography (OCTA) can image the retinal vasculature in vivo, without the need for contrast dye. This technology has been commercially available since 2014, however, much of its use has been limited to the research setting. Over time, more clinical practices have adopted OCTA imaging. While countless publications detail OCTA’s use for the study of retinal microvasculature, few studies outline OCTA’s clinical utility. Body This review provides an overview of OCTA imaging and details tips for successful interpretation. The review begins with a summary of OCTA technology and artifacts that arise from image acquisition. New methods and best practices to prevent image artifacts are discussed. OCTA has the unique ability among retinovascular imaging modalities to individually visualize each retinal plexus. Slabs offered in standard OCTA devices are reviewed, and clinical uses for each slab are outlined. Lastly, the use of OCTA for the clinical interpretation of retinal pathology, such as diabetic retinopathy and age-related macular degeneration, is discussed. Conclusion OCTA is evolving from a scientific tool to a clinical imaging device. This review provides a toolkit for successful image interpretation in a clinical setting.

Adaptive optics - optical coherence tomography for in vivo retinal imaging: effects of spectral bandwidth on image quality

2006 ◽  
Author(s):  
Robert J. Zawadzki ◽  
Steven M. Jones ◽  
Mingtao Zhao ◽  
Stacey S. Choi ◽  
Sophie S. Laut ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
Image Quality ◽  
Adaptive Optics ◽  
Retinal Imaging ◽  
Optical Coherence ◽  
Spectral Bandwidth

scholarly journals Megahertz-rate optical coherence tomography angiography improves the contrast of the choriocapillaris and choroid in human retinal imaging

2018 ◽  
Vol 10 (1) ◽  
pp. 50 ◽  
Author(s):  
Justin V. Migacz ◽  
Iwona Gorczynska ◽  
Mehdi Azimipour ◽  
Ravi Jonnal ◽  
Robert J. Zawadzki ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
Optical Coherence Tomography Angiography ◽  
Retinal Imaging ◽  
Optical Coherence

scholarly journals In vivo volumetric imaging of human retinal circulation with phase-variance optical coherence tomography

2011 ◽  
Vol 2 (6) ◽  
pp. 1504 ◽  
Author(s):  
Dae Yu Kim ◽  
Jeff Fingler ◽  
John S. Werner ◽  
Daniel M. Schwartz ◽  
Scott E. Fraser ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
Optical Coherence ◽  
Retinal Circulation ◽  
Volumetric Imaging ◽  
Phase Variance

scholarly journals Quantitative assessment of choriocapillaris flow deficits in diabetic retinopathy: A swept-source optical coherence tomography angiography study

PLoS ONE ◽  
2020 ◽  
Vol 15 (12) ◽  
pp. e0243830
Author(s):  
Yining Dai ◽  
Hao Zhou ◽  
Qinqin Zhang ◽  
Zhongdi Chu ◽  
Lisa C. Olmos de Koo ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
Diabetic Retinopathy ◽  
Healthy Subjects ◽  
Optical Coherence Tomography Angiography ◽  
Optical Coherence ◽  
Swept Source ◽  
Significant Difference ◽  
En Face ◽  
The Mean

Purpose To quantitatively assess choriocapillaris (CC) flow deficits in eyes with diabetic retinopathy (DR) using swept-source optical coherence tomography angiography (SS-OCTA). Methods Diabetic subjects with different stages of DR and age-matched healthy subjects were recruited and imaged with SS-OCTA. The en face CC blood flow images were generated using previously published and validated algorithms. The percentage of CC flow deficits (FD%) and the mean CC flow deficit size were calculated in a 5-mm-diameter circle centered on the fovea from the 6×6-mm scans. Results Forty-five diabetic subjects and 27 control subjects were included in the study. The CC FD% in diabetic eyes was on average 1.4-fold greater than in control eyes (12.34±4.14% vs 8.82±2.61%, P < 0.001). The mean CC FD size in diabetic eyes was on average 1.4-fold larger than in control eyes (2151.3± 650.8μm2 vs 1574.4±255.0 μm2, P < 0.001). No significant difference in CC FD% or mean CC FD size was observed between eyes with nonproliferative DR and eyes with proliferative DR (P = 1.000 and P = 1.000, respectively). Conclusions CC perfusion in DR can be objectively and quantitatively assessed with FD% and FD size. In the macular region, both CC FD% and CC FD size are increased in eyes with DR. SS-OCTA provides new insights for the investigations of CC perfusion status in diabetes in vivo.

scholarly journals Optical Coherence Tomography Angiography of Early Stage 1a Retinal Hemangioblastoma in Von-Hippel-Lindau

2021 ◽  
Vol 8 (3) ◽  
pp. 15-18
Author(s):  
Ananya Goswami ◽  
Abhidnya Surve ◽  
Pradeep Venkatesh
Keyword(s):  
Optical Coherence Tomography ◽  
Optical Coherence Tomography Angiography ◽  
Early Stage ◽  
Optical Coherence ◽  
Von Hippel Lindau ◽  
Retinal Capillary ◽  
Capillary Plexus ◽  
Retinal Hemangioblastoma ◽  
Retinal Capillaries

Von-Hippel-Lindau (VHL) syndrome is characterized by focal vasoproliferative tumors of retinal capillaries called retinal capillary hemangio-blastomas (RCH). These tumors are initially small and can be easily missed if not looked for carefully. As they grow, these tumors are more demanding to treat and hence the importance of detecting them early and treating them. Herein, we describe and review the optical coherence tomography angiography (OCTA) of the early-stage lesion, which suggested the involvement of superficial and a deeper retinal capillary plexus. In addition, to helping us detect these lesions earlier, OCTA may also help to understand the in vivo changes occurring at an earlier phase.

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