Reversible Regulation of Aptamer Activity with Effector-Responsive Hairpin Oligonucleotides

2013 ◽  
Vol 18 (1) ◽  
pp. 77-84
Author(s):  
Na Li
Keyword(s):  
Hairpin Oligonucleotides

One-step label-free chemiluminescent assay for determination of exonuclease III activity towards hairpin oligonucleotides

2019 ◽  
Vol 131 ◽  
pp. 109419
Author(s):  
Konstantin M. Burkin ◽  
Oleg L. Bodulev ◽  
Anastasia V. Gribas ◽  
Ivan Yu. Sakharov
Keyword(s):  
Label Free ◽  
Exonuclease Iii ◽  
Hairpin Oligonucleotides ◽  
One Step

Incorporation of a Non-Nucleotide Bridge into Hairpin Oligonucleotides Capable of High-Affinity Binding to the Rev Protein of HIV-1†

Biochemistry ◽  
1996 ◽  
Vol 35 (16) ◽  
pp. 5339-5344 ◽  
Author(s):  
Jeffrey S. Nelson ◽  
Lori Giver ◽  
Andrew D. Ellington ◽  
Robert L. Letsinger
Keyword(s):  
Affinity Binding ◽  
High Affinity Binding ◽  
High Affinity ◽  
Hairpin Oligonucleotides ◽  
Hiv 1 ◽  
Rev Protein

Factors influencing hairpin oligonucleotide cyclization by the uncatalyzed alkyne-azide cycloaddition (AAC) reaction

2010 ◽  
Vol 82 (8) ◽  
pp. 1599-1607 ◽  
Author(s):  
Afaf H. El-Sagheer ◽  
Tom Brown
Keyword(s):  
Sodium Chloride ◽  
Cycloaddition Reaction ◽  
Base Pairing ◽  
Factors Affecting ◽  
Alkyl Azides ◽  
Factors Influencing ◽  
Reaction Proceeds ◽  
Hairpin Oligonucleotides ◽  
Short Hairpin ◽  
Hairpin Oligonucleotide

Cyclization of short hairpin oligonucleotides (ODNs) containing unactivated alkynes and alkyl azides produces triazole-linked products slowly in the absence of CuI catalysis. The factors affecting the rate of this cycloaddition reaction have been investigated on a variety of hairpin sequences. The reaction proceeds best at intermediate concentrations of sodium chloride, but is inhibited by phosphate and Tris buffers. It is accelerated by increasing the temperature provided that Watson–Crick base pairing can occur. The rationale for these observations is discussed.

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