scholarly journals The relationships between the Golgi apparatus, GERL, and lysosomes of fetal rat liver Kupffer cells examined by ultrastructural phosphatase cytochemistry.

1981 ◽  
Vol 29 (9) ◽  
pp. 1061-1070 ◽  
Author(s):  
R M Pino ◽  
L C Pino ◽  
P W Bankston
Keyword(s):  
Golgi Apparatus ◽  
Kupffer Cells ◽  
Phosphatase Inhibitors ◽  
Golgi Region ◽  
Fetal Rat ◽  
Latex Spheres ◽  
Phosphatase Cytochemistry ◽  
Fetal Rat Liver ◽  
The Relationship ◽  
Rat Livers

Kupffer cells are the sinusoidal macrophages of the liver. Using ultrastructural phosphatase cytochemical methods, we examined the relationship between the Golgi apparatus, GERL, and lysosomes of Kupffer cells in fetal rat livers identified, in part, by their ability to phagocytize intravenously injected latex spheres. Thiamine pyrophosphatase (TPPase) activity was localized to the inner Golgi saccules and some vesicles in the Golgi region but not to GERL. A TPPase-like activity, demonstrable in lysosomes, was abolished by sodium fluoride but not suppressed by the alkaline phosphatase inhibitors L-cysteine and L-p-bromotetramisole. Acid phosphatase (AcPase) was localized by GERL, some coated vesicles, and in lysosomes, but not to the Golgi stacks. Continuities between GERL and lysosomes were observed. Phagosomes containing internalized latex spheres received TPPase and AcPase sequentially. TPPase was localized in phagosomes immediately after latex administration. AcPase activity was not found here until at least 10 minutes following the injection of the particulates. Our findings indicate that Kupffer cell lysosomes are derived from GERL, but also suggest that phagosomes may receive material packaged by the Golgi apparatus as well as GERL.

Endogenous Peroxidase Localization in Kupffer Cells of the Fetal Rat Liver

1977 ◽  
Vol 35 ◽  
pp. 424-425
Author(s):  
Richard M. Pino
Keyword(s):  
Rat Liver ◽  
Kupffer Cells ◽  
Wistar Rat ◽  
Adult Liver ◽  
Ether Anesthesia ◽  
Fetal Rat ◽  
Endogenous Peroxidase ◽  
Sinusoidal Endothelium ◽  
Fetal Rat Liver ◽  
Adult Bone

This study confirms the presence of endogenous peroxidase in fetal Kupffer cells. In adult hepatic sinusoids Kupffer cells are peroxidase positive; endothelial cells are not.1 The fetal rat liver sinusoidal endothelium during development changes from a lining similar to that found in adult bone marrow to an adult liver type.2 Cells that resemble adult Kupffer cells are evident at 13 days gestation and can endocytize carbon beginning at 14 days.Sixteen day Wistar rat fetuses were exposed in utero after ether anesthesia of their mothers. Colloidal carbon (Pelikan, Gunther Wagner) diluted 1:1 with double strength Tyrode1s solution, or as a control, single strength Tyrode's solution without carbon, was injected (0.01-0,03ml) into the fetal umbilical veins with a 30 gauge needle.2 After allowing one minute for the injected material to circulate, the fetuses were decapitated and the livers were removed and diced into small pieces.

scholarly journals Expression of c-Ki-ras, c-Ha-ras, and c-myc in specific cell types during hepatocarcinogenesis.

1985 ◽  
Vol 5 (4) ◽  
pp. 780-786 ◽  
Author(s):  
P Yaswen ◽  
M Goyette ◽  
P R Shank ◽  
N Fausto
Keyword(s):  
Rat Liver ◽  
Cell Types ◽  
Isolated Hepatocytes ◽  
Specific Cell ◽  
Oval Cells ◽  
Deficient Diet ◽  
Adult Rat ◽  
Fetal Rat ◽  
Fetal Rat Liver ◽  
Rat Livers

We examined the expression of six proto-oncogenes in (i) whole rat liver and isolated liver cell populations during the course of hepatocarcinogenesis induced by a choline-deficient diet containing 0.1% ethionine and (ii) fetal rat liver at different stages of development. The abundance of c-Ki-ras, c-Ha-ras, and c-myc transcripts in polysomal polyadenylated RNA from liver cells increased by 2 weeks after the start of the carcinogenic diet. c-Ki-ras and c-myc expression remained elevated during the 35 weeks of the diet, whereas c-Ha-ras transcripts increased transiently. A primary tumor sampled at 35 weeks after the carcinogenic diet was started contained high levels of both c-Ki-ras and c-myc RNA. The abundance of c-src transcripts was unchanged throughout carcinogenesis; c-abl and c-mos transcripts were not detected in either preneoplastic or neoplastic livers. To determine which cell types within the liver contained proto-oncogene transcripts, we isolated hepatocytes, oval cells, and bile duct cells from normal and preneoplastic livers. The results indicate that proto-oncogenes are expressed differentially in these cell types during hepatocarcinogenesis and that the expression of c-Ki-ras and c-myc is high in oval cells throughout carcinogenesis. In developing livers, c-Ki-ras, c-Ha-ras, and c-myc transcript levels were high at 17 days of gestation but reached the low values characteristic of adult rat livers between 20 days of gestation and 3 days after birth.

Expression of c-Ki-ras, c-Ha-ras, and c-myc in specific cell types during hepatocarcinogenesis

1985 ◽  
Vol 5 (4) ◽  
pp. 780-786
Author(s):  
P Yaswen ◽  
M Goyette ◽  
P R Shank ◽  
N Fausto
Keyword(s):  
Rat Liver ◽  
Cell Types ◽  
Isolated Hepatocytes ◽  
Specific Cell ◽  
Oval Cells ◽  
Deficient Diet ◽  
Adult Rat ◽  
Fetal Rat ◽  
Fetal Rat Liver ◽  
Rat Livers

We examined the expression of six proto-oncogenes in (i) whole rat liver and isolated liver cell populations during the course of hepatocarcinogenesis induced by a choline-deficient diet containing 0.1% ethionine and (ii) fetal rat liver at different stages of development. The abundance of c-Ki-ras, c-Ha-ras, and c-myc transcripts in polysomal polyadenylated RNA from liver cells increased by 2 weeks after the start of the carcinogenic diet. c-Ki-ras and c-myc expression remained elevated during the 35 weeks of the diet, whereas c-Ha-ras transcripts increased transiently. A primary tumor sampled at 35 weeks after the carcinogenic diet was started contained high levels of both c-Ki-ras and c-myc RNA. The abundance of c-src transcripts was unchanged throughout carcinogenesis; c-abl and c-mos transcripts were not detected in either preneoplastic or neoplastic livers. To determine which cell types within the liver contained proto-oncogene transcripts, we isolated hepatocytes, oval cells, and bile duct cells from normal and preneoplastic livers. The results indicate that proto-oncogenes are expressed differentially in these cell types during hepatocarcinogenesis and that the expression of c-Ki-ras and c-myc is high in oval cells throughout carcinogenesis. In developing livers, c-Ki-ras, c-Ha-ras, and c-myc transcript levels were high at 17 days of gestation but reached the low values characteristic of adult rat livers between 20 days of gestation and 3 days after birth.

scholarly journals The development of the sinusoids of fetal rat liver: localization of endogenous peroxidase in fetal Kupffer cells.

1979 ◽  
Vol 27 (2) ◽  
pp. 643-652 ◽  
Author(s):  
R M Pino ◽  
P W Bankston
Keyword(s):  
Rat Liver ◽  
Kupffer Cells ◽  
Serial Sections ◽  
Adult Liver ◽  
Monocytic Cells ◽  
Dense Bodies ◽  
Fetal Rat ◽  
Endogenous Peroxidase ◽  
Fetal Rat Liver ◽  
Cytochemical Marker

Endogenous peroxidase is the cytochemical marker used to identify Kupffer cells in the adult liver. In this study, we show by ultrastructural cytochemistry that Kupffer cells of the fetal rat liver are endogenous peroxidase positive. The reaction product is localized in the endoplasmic reticulum including the perinuclear cisternae and in a few lysosome-like dense bodies. Serial sections of Golgi regions suggest that GERL and not the Golgi stacks, is peroxidase positive. As in the adult liver, peroxidase is not localized in endothelial cells. Kupffer cells do not appear to transform from endothelial or extravascular developing monocytic cells and are present prior to bone marrow formation. The relevance of these observations with respect to the possible origin of the Kupffer cell is discussed.

Effects of Steroids on Fetal Rat Liver

1969 ◽  
Vol 27 ◽  
pp. 350-351
Author(s):  
F. G. Zaki
Keyword(s):  
Liver Injury ◽  
Rat Liver ◽  
Fetal Liver ◽  
Dosage Level ◽  
Maternal Plasma ◽  
Methyl Prednisolone ◽  
Fetal Rat ◽  
Toxic Agents ◽  
Fetal Rat Liver ◽  
Neonatal Liver

Fetal and neonatal liver injury induced by agents circulating in maternal plasma, even though well recognized, its morphological manifestations are not yet established. As part of our studies of fetal and neonatal liver injury induced by maternal nutritional disorders, metabolic impairment and toxic agents, the effects of two anti-inflammatory steroids have been recently inves tigated.Triamcinolone and methyl prednisolone were injected each in a group of rats during pregnancy at a-dosage level of 2 mgm three times a week. Fetal liver was studied at 18 days of gestation. Litter size and weight markedly decreased than those of control rats. Stillbirths and resorption were of higher incidence in the triamcinolone group than in those given the prednisolone.

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