Effects of Steroids on Fetal Rat Liver

1969 ◽  
Vol 27 ◽  
pp. 350-351
Author(s):  
F. G. Zaki
Keyword(s):  
Liver Injury ◽  
Rat Liver ◽  
Fetal Liver ◽  
Dosage Level ◽  
Maternal Plasma ◽  
Methyl Prednisolone ◽  
Fetal Rat ◽  
Toxic Agents ◽  
Fetal Rat Liver ◽  
Neonatal Liver

Fetal and neonatal liver injury induced by agents circulating in maternal plasma, even though well recognized, its morphological manifestations are not yet established. As part of our studies of fetal and neonatal liver injury induced by maternal nutritional disorders, metabolic impairment and toxic agents, the effects of two anti-inflammatory steroids have been recently inves tigated.Triamcinolone and methyl prednisolone were injected each in a group of rats during pregnancy at a-dosage level of 2 mgm three times a week. Fetal liver was studied at 18 days of gestation. Litter size and weight markedly decreased than those of control rats. Stillbirths and resorption were of higher incidence in the triamcinolone group than in those given the prednisolone.

Multiple forms of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase are expressed in perinatal rat liver

1996 ◽  
Vol 270 (2) ◽  
pp. E244-E250 ◽  
Author(s):  
M. Casado ◽  
L. Bosca ◽  
P. Martin-Sanz
Keyword(s):  
Liver Enzyme ◽  
Rat Liver ◽  
Fetal Liver ◽  
Ribonuclease Protection Assay ◽  
Adult Liver ◽  
Transcription Analysis ◽  
Fetal Rat ◽  
Protected Fragment ◽  
Fetal Rat Liver ◽  
Ribonuclease Protection

Fetal rat liver expresses a 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (PFK-2/Fru-2,6-Pase2) form that differs from the adult liver enzyme in the inhibition by phosphorylation by the adenosine 3',5'-cyclic monophosphate-dependent protein kinase and in the recognition by an antibody specific for the NH2-terminal domain of the adult liver enzyme. Northern blot analysis shows that fetal hepatocytes contain a species of mRNA that is 2.2 kb in size and that exhibits the maximal levels after delivery. PFK-2/Fru-2,6-Pase2 mRNA analysis using a sensitive ribonuclease protection assay reveals the presence of nearly similar amounts of adult liver-specific and skeletal muscle-specific mRNA in fetal liver and hepatocytes during the last days of gestation, as well as a 233-bp protected fragment present in fetal liver. These results were confirmed by polymerase chain reaction using specific oligonucleotide pairs. Primer extension of fetal liver cDNA suggests the presence of two initiation sites of transcription. Analysis of the adult liver PFK-2/Fru-2,6-Pase2 protein during the perinatal transition using a specific antibody shows a marked accumulation of this form immediately after birth.

scholarly journals Transplantation of Amniotic Epithelial Cells into Fetal Rat Liver by In Utero Manipulation

2002 ◽  
Vol 11 (5) ◽  
pp. 443-449 ◽  
Author(s):  
Nanae Takahashi ◽  
Shin Enosawa ◽  
Tasuku Mitani ◽  
Hua Lu ◽  
Seiichi Suzuki ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Rat Liver ◽  
Fetal Liver ◽  
Gene Carrier ◽  
Term Survival ◽  
Amniotic Epithelial Cells ◽  
Fetal Rat ◽  
Fetal Rat Liver ◽  
Number Of Cells

It has been hoped that amniotic epithelial cells would be a gene carrier to neural and hepatic tissue, because of 1) the presence of neural and hepatic stem-like cells, 2) the ability to cryopreserve them, 3) long-term survival in the transplanted site, and 4) few ethical problems concerning procurement. But transplantation of a sufficient number of cells to adult tissue needs large-scale cell supply and may lead to vascular embolism. We attempted transplantation of amniotic epithelial cells into fetal liver, because 1) the fetal liver is at the proliferative stage, 2) the number of cells required is small, and 3) the fetal stage is advantageous for the induction of immunological tolerance. Amniotic epithelial cells from day 18.5–20.5 fetuses were transfected with adenoviral AdlacZ and harvested to inject into fetal rat liver of the syngeneic strain (day 18.5–20.5). The efficacy of cell transplantation into the liver increased in the order: intraplacental < intraumbilical vein < intrahepatic route. LacZ-transfected amniotic cells (1–8 × 105 cells), hepatocytes (5 × 105 cells), or AdlacZ vector solution (1.7 × 107 pfu) were injected through the uterine membrane into the liver. Transplanted cells formed a cellular mass and survived for up to 14 days after birth, whereas lacZ-transfected cells were rapidly decreased after the injection of AdlacZ vector or rat hepatocytes as a gene carrier so that the use of amniotic epithelial cells as a gene carrier will result in long-term expression of exogenous genes in the liver.

Influence of age, adrenalectomy, and corticosteroids on hepatic transaminase activity

1961 ◽  
Vol 201 (2) ◽  
pp. 271-275 ◽  
Author(s):  
Homer R. Harding ◽  
Fred Rosen ◽  
Charles A. Nichol
Keyword(s):  
Rat Liver ◽  
Specific Activity ◽  
Fetal Liver ◽  
Alanine Transaminase ◽  
Female Rats ◽  
Male Rats ◽  
Transaminase Activity ◽  
Fetal Rat ◽  
Hepatic Transaminase ◽  
Fetal Rat Liver

The activity of alanine-α-ketoglutarate transaminase in rat liver was found to be uniform during the first 6 weeks of life, increasing thereafter until at 48 weeks of age the specific activity was seven times greater than in the immature rat. In rats varying in age from 4 days to 24 weeks, treatment with 1 mg of cortisol for 4 days resulted in significant increases in hepatic alanine transaminase activity. Female rats were less responsive to cortisol treatment than were males. Fetal rat liver had significantly lower alanine transaminase activity than did newborn animals and the activity of this enzyme in fetal liver was not altered by cortisol treatment. Adrenalectomy of adult male rats, but not immature male rats, resulted in a decrease in alanine transaminase activity; after 48 hr, enzyme activity was depressed to levels found in unoperated immature rats. The sensitivity of the adrenalectomized animals to cortisol administration was comparable to that of intact animals. In contrast, the aspartate-α-ketoglutarate transaminase was not appreciably altered following cortisol treatment or adrenalectomy and did not increase with age.

Changes in the properties of fetal rat liver during organ culture

In Vitro ◽  
1979 ◽  
Vol 15 (8) ◽  
pp. 579-586
Author(s):  
Carl Monder ◽  
Alena Hatle Coufalik
Keyword(s):  
Organ Culture ◽  
Rat Liver ◽  
Fetal Rat ◽  
Fetal Rat Liver

Hormonal control of fructose 2,6-bisphosphate concentration and of phosphofructokinase 2 in the rat liver during development

1986 ◽  
Vol 6 (6) ◽  
pp. 513-518 ◽  
Author(s):  
Christian Schubert ◽  
Hans-Joachim Boehme ◽  
Eberhard Hofmann
Keyword(s):  
Rat Liver ◽  
Total Activity ◽  
Hormonal Control ◽  
Developmental Changes ◽  
Adrenal Hormones ◽  
Phosphorylation State ◽  
Exogenous Glucose ◽  
Substrate Supply ◽  
Fetal Rat ◽  
Fetal Rat Liver

In fetal rat liver the concentration of fructose 2,6-bisphosphate is decreased by administration of glucagon. The glucagon effect, i.e., the phosphorylation state of phosphofructokinase 2, dominates over the substrate supply. Insulin was found to increase fructose 2,6-bisphosphate only when exogenous glucose is supplied simultaneously. The total activity of phosphofructokinase 2 exhibits remarkable developmental changes. It is high at term, moderate in the fetal as well as in the mature organ, and low during suckling. The level of the enzyme during development is controlled by pancreatic and adrenal hormones.

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