Localization of calcitonin and calcitonin gene-related peptide mRNAs in rat parafollicular cells by hybridocytochemistry.
1988 ◽
Vol 36
(5)
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pp. 543-546
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Keyword(s):
Dot Blot
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By use of appropriate fragments of CT DNA or a CGRP DNA and SP6 polymerase system, we produced anti-sense RNA probes labeled with biotinylated 11-UTP. The labeling and specificity of the RNA probes were confirmed using dot-blot hybridization. By use of hybridocytochemistry, CT mRNA and CGRP mRNA were localized in all parafollicular cells in control and dihydrotachysterin-pre-treated rats. We concluded that all parafollicular cells simultaneously produce both CT mRNA and CGRP mRNA, either under control conditions or after stimulation by dihydrotachysterin-induced hypercalcemia.
1989 ◽
Vol 256
(5)
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pp. E597-E599
2001 ◽
Vol 8
(3)
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pp. 165-168
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Keyword(s):
1997 ◽
Vol 4
(1)
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pp. 15-21
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1998 ◽
Vol 5
(1)
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pp. 73A-73A
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1996 ◽
Vol 217
(2-3)
◽
pp. 165-168
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Keyword(s):
Keyword(s):