scholarly journals Co60 Vitamin B12 Binding Capacity of Human Leukocytes

Blood ◽  
1962 ◽  
Vol 19 (2) ◽  
pp. 229-235 ◽  
Author(s):  
LEO M. MEYER ◽  
EUGENE P. CRONKITE ◽  
INEZ F. MILLER ◽  
CLAIRE W MULZAC ◽  
IRVING JONES
Keyword(s):  
Vitamin B12 ◽  
Human Serum ◽  
Polycythemia Vera ◽  
Binding Capacity ◽  
Chronic Myelocytic Leukemia ◽  
Nonspecific Binding ◽  
Two Phase ◽  
Intact Cells ◽  
Myelocytic Leukemia ◽  
Neutrophilic Leukocytes

Abstract 1. Mature neutrophilic leukocytes show the highest Co60B12 binding capacity. 2. Less mature granulocytes, "blast" forms and eosinophils have little or no Co60B12 binding capacity. 3. Disintegrated mature leukocytes from chronic myelocytic leukemia and polycythemia vera show higher B12 binding capacity than intact cells. 4. Mature leukocytes from patients with chronic myelocytic leukemia and polycythemia vera show a two-phase B12 curve suggesting specific and nonspecific binding, similar to that observed in human serum. 5. Disintegration products from mature neutrophilic leukocytes probably contribute largely to increased B12 binding capacity of serum in chronic myelocytic leukemia and polycythemia vera.

scholarly journals The Kinetics of Intravenously Injected Radioactive Vitamin B12: Studies on Normal Subjects and Patients with Chronic Myelocytic Leukemia and Pernicious Anemia

Blood ◽  
1960 ◽  
Vol 15 (5) ◽  
pp. 646-661 ◽  
Author(s):  
EUGENE A. BRODY ◽  
SOLOMON ESTREN ◽  
LOUIS R. WASSERMAN
Keyword(s):  
Vitamin B12 ◽  
Pernicious Anemia ◽  
Total Gastrectomy ◽  
Binding Capacity ◽  
Intrinsic Factor ◽  
Normal Subjects ◽  
High Serum ◽  
Malabsorption Syndrome ◽  
Chronic Myelocytic Leukemia ◽  
Myelocytic Leukemia

Abstract 1. Studies of the fate of intravenously injected radioactive vitamin B12 have been performed in patients with normal, low and high serum concentrations of vitamin B12. 2. Abnormal plasma disappearance curves were noted in chronic myelocytic leukemia, pernicious anemia in relapse and in remission, total gastrectomy and malabsorption syndrome. 3. In chronic myelocytic leukemia, the slow clearance of plasma radioactivity may be explained by the increased binding capacity of the plasma proteins for vitamin B12. 4. Plasma clearance of radioactivity is slower than normal in pernicious anemia, even in remission. The failure of the disappearance curve to return to normal in pernicious anemia in complete remission suggests the existence of a plasma "B12-transferase," whose function is to transfer circulating B12 to the tissues. The disappearance curves suggest that the amount of such "B12-transferase" is diminished in pernicious anemia, total gastrectomy and certain Cases of malabsorption syndrome. 5. A relationship between a hypothetical "B12-transferase" and intrinsic factor is discussed.

scholarly journals Effect of Myleran and 6-Mercaptopurine (6-MP) on the Serum Level of Vitamin B12 in Chronic Myelocytic Leukemia

Blood ◽  
1959 ◽  
Vol 14 (3) ◽  
pp. 274-278 ◽  
Author(s):  
V. M. DOCTOR ◽  
D. E. BERGSAGEL ◽  
C. C. SHULLENBERGER
Keyword(s):  
Serum Level ◽  
Vitamin B12 ◽  
Serum Vitamin ◽  
Chronic Myelocytic Leukemia ◽  
Myelocytic Leukemia ◽  
Leukocyte Counts

Abstract Serum vitamin B12 determinations and the total leukocyte counts were made at regular intervals in chronic myelocytic leukemia patients before, during and after therapy with 6-MP or Myleran. The results indicate that a reduction in total leukocytes following therapy is followed by a gradual lowering of serum vitamin B12. The levels of serum vitamin B12 at the completion of therapy may be related to the duration of remission.

scholarly journals Biochemical Studies in Chronic Myelocytic Leukemia, Polycythemia Vera and Other Idiopathic Myeloproliferative Disorders

Blood ◽  
1952 ◽  
Vol 7 (10) ◽  
pp. 959-977 ◽  
Author(s):  
WILLIAM N. VALENTINE ◽  
WILLIAM S. BECK ◽  
JAMES H. FOLLETTE ◽  
HAROLD MILLS ◽  
JOHN S. LAWRENCE
Keyword(s):  
Polycythemia Vera ◽  
Myeloproliferative Disorders ◽  
Chronic Myelocytic Leukemia ◽  
Myelocytic Leukemia ◽  
Biochemical Studies

scholarly journals The Plasma Clearance and Urinary Excretion of Parenterally Administered 58Co B12

Blood ◽  
1956 ◽  
Vol 11 (1) ◽  
pp. 31-43 ◽  
Author(s):  
D. L. MOLLIN ◽  
W. R. PITNEY ◽  
S. J. BAKER ◽  
J. E. BRADLEY
Keyword(s):  
Urinary Excretion ◽  
Vitamin B12 ◽  
Plasma Clearance ◽  
Vitamin B12 Deficiency ◽  
Normal Subjects ◽  
Normal Serum ◽  
Chronic Myelocytic Leukemia ◽  
Intravenous Injections ◽  
Myelocytic Leukemia ◽  
B12 Deficiency

Abstract Intravenous injections of 1.5 µg. of 58Co B12 were given to subjects with normal serum B12 concentrations, to patients with vitamin B12 deficiency and to patients with chronic myelocytic leukemia. The rate of plasma clearance of radioactivity after this dose was slowest in patients with chronic myelocytic leukemia and patients with pernicious anemia in severe relapse. In patients with vitamin B12 deficiency, serum B12 concentrations were estimated microbiologically at frequent intervals after the injection. There was a good correlation between the results obtained by microbiological assay and as calculated from plasma radioactivity. Significant differences were not observed between the urinary excretion of radioactivity by normal subjects and patients with B12 deficiency.

scholarly journals Identification of the Vitamin B12-Binding Protein in the Serum of Normals and of Patients with Chronic Myelocytic Leukemia

Blood ◽  
1958 ◽  
Vol 13 (8) ◽  
pp. 740-747 ◽  
Author(s):  
ROBERT S. MENDELSOHN ◽  
DONALD M. WATKIN ◽  
ANN P. HORBETT ◽  
JOHN L. FAHEY
Keyword(s):  
Vitamin B12 ◽  
Binding Protein ◽  
Qualitative Difference ◽  
Normal Subjects ◽  
Paper Electrophoresis ◽  
Normal Serum ◽  
Chronic Myelocytic Leukemia ◽  
Myelocytic Leukemia ◽  
Abnormal Metabolism

Abstract Vitamin B12-binding proteins in the serum of normal subjects and of patients with chronic myelocytic leukemia have been compared. The in-vivo-bound B12 was utilized to identify the binding protein. Column protein chromatography and block and paper electrophoresis were employed individually and in combination to characterize the B12-binding protein. B12 was found to be bound primarily to an alpha-l globulin in both normal individuals and in patients with chronic myelocytic leukemia. No qualitative difference was found in these proteins. The increased amounts of B12-binding protein in the serum of patients with chronic myelocytic leukemia would seem to be attributable to abnormal metabolism of the same protein that binds B12 in normal serum.

scholarly journals Comparison of Chromosome Constitution in Chronic Myelocytic Leukemia and Other Myeloproliferative Disorders

Blood ◽  
1962 ◽  
Vol 20 (4) ◽  
pp. 393-423 ◽  
Author(s):  
AVERY A. SANDBERG ◽  
TAKAAKI ISHIHARA ◽  
LOIS H. CROSSWHITE ◽  
THEODORE S. HAUSCHKA
Keyword(s):  
Polycythemia Vera ◽  
Blood Cells ◽  
Chromosome Abnormality ◽  
Myeloproliferative Disorders ◽  
Chronic Myelocytic Leukemia ◽  
Myeloid Metaplasia ◽  
Myelocytic Leukemia ◽  
Karyotypic Abnormality ◽  
Blastic Phase ◽  
Group Chromosome

Abstract The frequency of the Ph1 chromosome in freshly aspirated marrow cells of 14 patients with typical chronic myelocytic leukemia processed by a "direct technic" without resort to culture or colchicine was significantly higher (> 75 per cent) than that observed in the cultured blood cells (< 35 per cent) of the same subjects. The karyotypic abnormally of the abbreviated G-group chromosome would appear not to be related to therapy, since the frequency with which it occurred was not materially affected by treatment (including radiation). The Ph1 chromosome was not observed in any of the metaphases of blood or marrow of 12 subjects who had developed a leukemia-like picture complicating either myelofibrosis, polycythemia vera or myeloid metaplasia. A new chromosome abnormality—a shortened D-group chromosome—was observed with about the same frequency in the blood and marrow metaphases of a female patient with treated chronic myelocytic leukemia. This new karyotypic abnormality was associated with the highest frequency of the Ph1 chromosome in cultured blood cells in the group studied. The Ph1 chromosome was observed in the metaphases of a patient with the blastic phase of chronic myelocytic leukemia. The variations of the morphology of the Ph1 chromosome are discussed and illustrated, especially in relation to the Y-chromosome. In four patients with an atypical picture of CML, the Ph1 chromosome was not observed either in the marrow or cultured blood.

scholarly journals Granulocyte Kinetic Studies in Patients with Proliferative Disorders of the Bone Marrow

Blood ◽  
1963 ◽  
Vol 22 (2) ◽  
pp. 125-138 ◽  
Author(s):  
ALVIN M. MAUER ◽  
THOMAS JARROLD
Keyword(s):  
Polycythemia Vera ◽  
Essential Thrombocythemia ◽  
Myeloid Cells ◽  
Kinetic Studies ◽  
Normal Subjects ◽  
Chronic Myelocytic Leukemia ◽  
Turnover Rates ◽  
Myelocytic Leukemia ◽  
Blood Granulocyte ◽  
Uniform Population

Abstract Granulocyte kinetic studies with DFP32 were done in four patients with chronic myelocytic leukemia, three patients with polycythemia vera, one patient with essential thrombocythemia, and one patient with persistent, unexplained granulocytosis. The increased blood granulocyte concentration found in the patients with polycythemia vera, essential thrombocythemia and unexplained granulocytosis was at least in part the result of increased granulocyte production. Precise calculations of granulocyte pool sizes and turnover rates in the patients with chronic myelocytic leukemia were not possible because of unresolved problems related to the non-uniform population of myeloid cells in the blood of these patients. However, within the limitations of the method, a greater number of myeloid cells were turned over per day through blood than in normal subjects. The findings support the concept that a widespread disorder of marrow proliferation exists in chronic myelocytic leukemia, polycythemia vera, and essential thrombocythemia.

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