scholarly journals Biochemical Studies in Chronic Myelocytic Leukemia, Polycythemia Vera and Other Idiopathic Myeloproliferative Disorders

Blood ◽  
1952 ◽  
Vol 7 (10) ◽  
pp. 959-977 ◽  
Author(s):  
WILLIAM N. VALENTINE ◽  
WILLIAM S. BECK ◽  
JAMES H. FOLLETTE ◽  
HAROLD MILLS ◽  
JOHN S. LAWRENCE
Keyword(s):  
Polycythemia Vera ◽  
Myeloproliferative Disorders ◽  
Chronic Myelocytic Leukemia ◽  
Myelocytic Leukemia ◽  
Biochemical Studies

scholarly journals Comparison of Chromosome Constitution in Chronic Myelocytic Leukemia and Other Myeloproliferative Disorders

Blood ◽  
1962 ◽  
Vol 20 (4) ◽  
pp. 393-423 ◽  
Author(s):  
AVERY A. SANDBERG ◽  
TAKAAKI ISHIHARA ◽  
LOIS H. CROSSWHITE ◽  
THEODORE S. HAUSCHKA
Keyword(s):  
Polycythemia Vera ◽  
Blood Cells ◽  
Chromosome Abnormality ◽  
Myeloproliferative Disorders ◽  
Chronic Myelocytic Leukemia ◽  
Myeloid Metaplasia ◽  
Myelocytic Leukemia ◽  
Karyotypic Abnormality ◽  
Blastic Phase ◽  
Group Chromosome

Abstract The frequency of the Ph1 chromosome in freshly aspirated marrow cells of 14 patients with typical chronic myelocytic leukemia processed by a "direct technic" without resort to culture or colchicine was significantly higher (> 75 per cent) than that observed in the cultured blood cells (< 35 per cent) of the same subjects. The karyotypic abnormally of the abbreviated G-group chromosome would appear not to be related to therapy, since the frequency with which it occurred was not materially affected by treatment (including radiation). The Ph1 chromosome was not observed in any of the metaphases of blood or marrow of 12 subjects who had developed a leukemia-like picture complicating either myelofibrosis, polycythemia vera or myeloid metaplasia. A new chromosome abnormality—a shortened D-group chromosome—was observed with about the same frequency in the blood and marrow metaphases of a female patient with treated chronic myelocytic leukemia. This new karyotypic abnormality was associated with the highest frequency of the Ph1 chromosome in cultured blood cells in the group studied. The Ph1 chromosome was observed in the metaphases of a patient with the blastic phase of chronic myelocytic leukemia. The variations of the morphology of the Ph1 chromosome are discussed and illustrated, especially in relation to the Y-chromosome. In four patients with an atypical picture of CML, the Ph1 chromosome was not observed either in the marrow or cultured blood.

scholarly journals Co60 Vitamin B12 Binding Capacity of Human Leukocytes

Blood ◽  
1962 ◽  
Vol 19 (2) ◽  
pp. 229-235 ◽  
Author(s):  
LEO M. MEYER ◽  
EUGENE P. CRONKITE ◽  
INEZ F. MILLER ◽  
CLAIRE W MULZAC ◽  
IRVING JONES
Keyword(s):  
Vitamin B12 ◽  
Human Serum ◽  
Polycythemia Vera ◽  
Binding Capacity ◽  
Chronic Myelocytic Leukemia ◽  
Nonspecific Binding ◽  
Two Phase ◽  
Intact Cells ◽  
Myelocytic Leukemia ◽  
Neutrophilic Leukocytes

Abstract 1. Mature neutrophilic leukocytes show the highest Co60B12 binding capacity. 2. Less mature granulocytes, "blast" forms and eosinophils have little or no Co60B12 binding capacity. 3. Disintegrated mature leukocytes from chronic myelocytic leukemia and polycythemia vera show higher B12 binding capacity than intact cells. 4. Mature leukocytes from patients with chronic myelocytic leukemia and polycythemia vera show a two-phase B12 curve suggesting specific and nonspecific binding, similar to that observed in human serum. 5. Disintegration products from mature neutrophilic leukocytes probably contribute largely to increased B12 binding capacity of serum in chronic myelocytic leukemia and polycythemia vera.

scholarly journals Granulocyte Kinetic Studies in Patients with Proliferative Disorders of the Bone Marrow

Blood ◽  
1963 ◽  
Vol 22 (2) ◽  
pp. 125-138 ◽  
Author(s):  
ALVIN M. MAUER ◽  
THOMAS JARROLD
Keyword(s):  
Polycythemia Vera ◽  
Essential Thrombocythemia ◽  
Myeloid Cells ◽  
Kinetic Studies ◽  
Normal Subjects ◽  
Chronic Myelocytic Leukemia ◽  
Turnover Rates ◽  
Myelocytic Leukemia ◽  
Blood Granulocyte ◽  
Uniform Population

Abstract Granulocyte kinetic studies with DFP32 were done in four patients with chronic myelocytic leukemia, three patients with polycythemia vera, one patient with essential thrombocythemia, and one patient with persistent, unexplained granulocytosis. The increased blood granulocyte concentration found in the patients with polycythemia vera, essential thrombocythemia and unexplained granulocytosis was at least in part the result of increased granulocyte production. Precise calculations of granulocyte pool sizes and turnover rates in the patients with chronic myelocytic leukemia were not possible because of unresolved problems related to the non-uniform population of myeloid cells in the blood of these patients. However, within the limitations of the method, a greater number of myeloid cells were turned over per day through blood than in normal subjects. The findings support the concept that a widespread disorder of marrow proliferation exists in chronic myelocytic leukemia, polycythemia vera, and essential thrombocythemia.

scholarly journals Bone Marrow in Polycythemia Vera, Chronic Myelocytic Leukemia, and Myelofibrosis Has an Increased Vascularity

2000 ◽  
Vol 157 (1) ◽  
pp. 15-19 ◽  
Author(s):  
Lars Göran Lundberg ◽  
Richard Lerner ◽  
Pär Sundelin ◽  
Rick Rogers ◽  
Judah Folkman ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Polycythemia Vera ◽  
Chronic Myelocytic Leukemia ◽  
Myelocytic Leukemia

scholarly journals Simultaneous genotypic and immunophenotypic analysis of interphase cells using dual-color fluorescence: a demonstration of lineage involvement in polycythemia vera

Blood ◽  
1992 ◽  
Vol 80 (4) ◽  
pp. 1033-1038 ◽  
Author(s):  
CM Price ◽  
EJ Kanfer ◽  
SM Colman ◽  
N Westwood ◽  
AJ Barrett ◽  
...  
Keyword(s):  
Polycythemia Vera ◽  
Fluorescent In Situ Hybridization ◽  
Chromosomal Abnormalities ◽  
Myeloproliferative Disorders ◽  
Chromosome 8 ◽  
Dual Fluorescence ◽  
Interphase Cell ◽  
Molecular Alterations ◽  
Interphase Cells

Abstract Fluorescent in situ hybridization has become a useful technique by which chromosomal abnormalities may be shown in interphase cells. We present a dual-fluorescence method whereby a chromosomal and immunophenotypic marker can be visualized simultaneously in the same interphase cell. Two patients with the myeloproliferative disorder polycythemia vera and trisomy for chromosome 8 have been studied using this technique and selective involvement of the myeloid and erythrocyte lineages has been shown by the detection of the trisomy in immunophenotyped cells. Simultaneous analysis of genotype and immunophenotype in individual cells from patients with myeloproliferative disorders or leukemia may help identify the developmental and lineage status of cells in which molecular alterations have resulted in clonal advantage.

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