scholarly journals A "Philadelphia-like" Chromosome Derived From the Y in a Patient With Refractory Dysplastic Anemia

Blood ◽  
1973 ◽  
Vol 42 (5) ◽  
pp. 799-804 ◽  
Author(s):  
Dorothy Warburton ◽  
Avrum Bluming
Keyword(s):  
Y Chromosome ◽  
Peripheral Blood ◽  
Acrocentric Chromosome ◽  
Marrow Cell ◽  
Philadelphia Chromosome ◽  
Peripheral Blood Lymphocytes ◽  
Chronic Myelocytic Leukemia ◽  
Fluorescent Staining ◽  
Blood Lymphocytes ◽  
Myelocytic Leukemia

Abstract A small acrocentric chromosome, identical in appearance to the Philadelphia chromosome of chronic myelocytic leukemia, was identified in all karyotyped marrow cell metaphases prepared from a 74-yr-old male with refractory dysplastic anemia. The abnormal chromosome was shown by quinacrine fluorescent staining to be derived from a Y chromosome which has lost the characteristic brilliantly fluorescent material of its long arms. A normal Y chromosome was consistently observed in karyotypes of cultured peripheral blood lymphocytes from this patient.

Rapid Y-chromosome-assay sexing of peripheral blood lymphocytes from bovinae of known phenotypic sex

1990 ◽  
Vol 33 (1) ◽  
pp. 246 ◽  
Author(s):  
Charles M. Herr ◽  
Klaus I. Matthaei ◽  
Trevor Steel ◽  
Ken C. Reed
Keyword(s):  
Y Chromosome ◽  
Peripheral Blood ◽  
Peripheral Blood Lymphocytes ◽  
Blood Lymphocytes

scholarly journals Chronic Myelocytic Leukemia in Identical Twins and a Sibling

Blood ◽  
1968 ◽  
Vol 31 (2) ◽  
pp. 216-225 ◽  
Author(s):  
GEORGE K. TOKUHATA ◽  
CHARLES L. NEELY ◽  
DOROTHY L. WILLIAMS
Keyword(s):  
Peripheral Blood ◽  
Blood Cells ◽  
Genetic Factor ◽  
Philadelphia Chromosome ◽  
Chromosome Abnormalities ◽  
Identical Twins ◽  
Peripheral Blood Cells ◽  
Chronic Myelocytic Leukemia ◽  
Myelocytic Leukemia

Abstract Identical twins and their older brother have been studied: all diagnosed within a span of three months as having chronic myelocytic leukemia; both twins were symptomatic and the brother asymptomatic. Chromosome analyses were made on peripheral blood cells. The asymptomatic brother had never been treated. The Ph1 chromosome was present in each of the three siblings. A number of other chromosome abnormalities were found. Results were interpreted in terms of a probable genetic factor in the Philadelphia chromosome and susceptibility to chronic myelocytic leukemia.

scholarly journals Erythroid colony stimulating and inhibiting cells in peripheral blood of transfused dogs: separation of function by velocity sedimentation

Blood ◽  
1979 ◽  
Vol 54 (4) ◽  
pp. 955-958 ◽  
Author(s):  
B Torok-Storb ◽  
HJ Deeg ◽  
K Atkinson ◽  
PL Weiden ◽  
JW Adamson ◽  
...  
Keyword(s):  
Peripheral Blood ◽  
Marrow Cell ◽  
Peripheral Blood Lymphocytes ◽  
Cell Interactions ◽  
Velocity Sedimentation ◽  
Narrow Peak ◽  
Blood Lymphocytes ◽  
Opposing Effects ◽  
Allogeneic Marrow

Abstract We have previously shown that the addition of normal dog peripheral blood lymphocytes (PBL) to cultures of allogeneic marrow increases the number of marrow-derived erythroid colonies (EC), but that PBL from transfused dogs usually inhibit EC growth from marrow of the transfusion donor. In this study, the cells in normal dog PBL responsible for stimulating EC growth were shown to sediment in a narrow peak at 4.30 mm/hr. A similar population of stimulating cells exists in transfused dogs and can be separated, on the basis of size, from cells that inhibit EC growth. EC-stimulating cells from transfused dog PBL sediment at 3.3--5.0 mm/hr, while cells responsible for inhibition are larger and sediment more rapidly at 5.4--8.1 mm/hr. These data demonstrate that cells capable of stimulating allogeneic EC are present in transfused dogs, but their stimulating ability is masked by the presence of EC-inhibiting cells. Thus, coculture experiments designed to test lymphocyte/marrow cell interactions may miss significant but opposing effects if unfractionated cells are used.

Erythroid colony stimulating and inhibiting cells in peripheral blood of transfused dogs: separation of function by velocity sedimentation

Blood ◽  
1979 ◽  
Vol 54 (4) ◽  
pp. 955-958
Author(s):  
B Torok-Storb ◽  
HJ Deeg ◽  
K Atkinson ◽  
PL Weiden ◽  
JW Adamson ◽  
...  
Keyword(s):  
Peripheral Blood ◽  
Marrow Cell ◽  
Peripheral Blood Lymphocytes ◽  
Cell Interactions ◽  
Velocity Sedimentation ◽  
Narrow Peak ◽  
Blood Lymphocytes ◽  
Opposing Effects ◽  
Allogeneic Marrow

We have previously shown that the addition of normal dog peripheral blood lymphocytes (PBL) to cultures of allogeneic marrow increases the number of marrow-derived erythroid colonies (EC), but that PBL from transfused dogs usually inhibit EC growth from marrow of the transfusion donor. In this study, the cells in normal dog PBL responsible for stimulating EC growth were shown to sediment in a narrow peak at 4.30 mm/hr. A similar population of stimulating cells exists in transfused dogs and can be separated, on the basis of size, from cells that inhibit EC growth. EC-stimulating cells from transfused dog PBL sediment at 3.3--5.0 mm/hr, while cells responsible for inhibition are larger and sediment more rapidly at 5.4--8.1 mm/hr. These data demonstrate that cells capable of stimulating allogeneic EC are present in transfused dogs, but their stimulating ability is masked by the presence of EC-inhibiting cells. Thus, coculture experiments designed to test lymphocyte/marrow cell interactions may miss significant but opposing effects if unfractionated cells are used.

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