scholarly journals Comparison of the hemagglutination inhibition assay kit for erythropoietin (ESF) with the fetal mouse liver cell bioassay in vitro

Blood ◽  
1980 ◽  
Vol 55 (6) ◽  
pp. 955-959
Author(s):  
G de Klerk ◽  
RJ Vet ◽  
PC Rosengarten ◽  
R Goudsmit

The commercially available hemagglutination inhibition (HAI) assay kit for erythropoietin (ESF) was compared with the fetal mouse liver cell (FMLC) bioassay. No correlation was obtained ESF levels determined by both methods in a variety of pathologic sera. The HAI kit showed a great batch variability. Significant immunoreactivity was found in those fractions of a normal human serum and a human urinary ESF preparation that were not active in the FMLC bioassay. A very poor recovery of immunoreactivity was found when the international reference preparation for erythropoietin (second IRPE) was added to a normal human serum.

Blood ◽  
1980 ◽  
Vol 55 (6) ◽  
pp. 955-959 ◽  
Author(s):  
G de Klerk ◽  
RJ Vet ◽  
PC Rosengarten ◽  
R Goudsmit

Abstract The commercially available hemagglutination inhibition (HAI) assay kit for erythropoietin (ESF) was compared with the fetal mouse liver cell (FMLC) bioassay. No correlation was obtained ESF levels determined by both methods in a variety of pathologic sera. The HAI kit showed a great batch variability. Significant immunoreactivity was found in those fractions of a normal human serum and a human urinary ESF preparation that were not active in the FMLC bioassay. A very poor recovery of immunoreactivity was found when the international reference preparation for erythropoietin (second IRPE) was added to a normal human serum.


Blood ◽  
1978 ◽  
Vol 52 (6) ◽  
pp. 1238-1242
Author(s):  
CD Dunn ◽  
RD Lange

Titers of erythroid regulatory factors (ERF), as measured in vivo with a standard erythrocythemic mouse technique, showed a highly significant correlation with those obtained in vitro with a fetal mouse liver cell (FMLC) assay. The results from the assay in vitro were somewhat higher than would be predicted from the assay in vivo. Nevertheless, the FMLC technique has been found to be a valid and useful technique particularly in the study of serum ERF titers.


Blood ◽  
1978 ◽  
Vol 52 (6) ◽  
pp. 1238-1242 ◽  
Author(s):  
CD Dunn ◽  
RD Lange

Abstract Titers of erythroid regulatory factors (ERF), as measured in vivo with a standard erythrocythemic mouse technique, showed a highly significant correlation with those obtained in vitro with a fetal mouse liver cell (FMLC) assay. The results from the assay in vitro were somewhat higher than would be predicted from the assay in vivo. Nevertheless, the FMLC technique has been found to be a valid and useful technique particularly in the study of serum ERF titers.


1998 ◽  
Vol 18 (5) ◽  
pp. 355-358 ◽  
Author(s):  
Masami Matsumura ◽  
Syuichi Hatakeyama ◽  
Ichiro Koni ◽  
Hiroshi Mabuchi

1969 ◽  
Vol 24 (11) ◽  
pp. 1442-1447 ◽  
Author(s):  
J. J. Picard ◽  
J. F. Heremans

The colloidal dye lithium carmine was added in vitro to normal human serum. Electrophoretic experiments showed that the dye was associated mainly with α2-globulins, small amounts with the albumin and only traces with the γ-globulins. The main complex was eluted with the macroglobulin peak obtained by gel filtration on Sephadex G-200 and sedimented in the heavy fraction on density gradient ultracentrifugation. The dye-protein complex could be precipitated with an antiserum specific for a2-macroglobulin. Gel filtration of a solution of pure a2-macroglobulin, to which lithium carmine was added, demonstrated that the dye was bound to this protein.


1982 ◽  
Vol 12 (2) ◽  
pp. 179-186 ◽  
Author(s):  
Takahiro Okamoto ◽  
Akihisa Kanamaru ◽  
Hiroshi Hara ◽  
Kiyoyasu Nagai

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