scholarly journals The Incidence and Natural Course of Transfusion-Associated GB Virus C/Hepatitis G Virus Infection in a Cohort of Thalassemic Patients

Blood ◽  
1998 ◽  
Vol 91 (3) ◽  
pp. 774-777 ◽  
Author(s):  
Daniele Prati ◽  
Alberto Zanella ◽  
Patrizia Bosoni ◽  
Paolo Rebulla ◽  
Elena Farma ◽  
...  
Keyword(s):  
Natural Course ◽  
Chain Reaction ◽  
Serum Samples ◽  
Hepatitis G Virus ◽  
Gb Virus C ◽  
Polymerase Chain ◽  
Hepatitis G ◽  
Virus C ◽  
A Prospective Study

To evaluate the risk of transmitting blood-borne GB virus C/hepatitis G virus (GBV-C/HGV) and to define the natural course of infection, we performed a prospective study in a cohort of multitransfused β-thalassemics during a 6-year follow-up period. We analyzed serum samples of 150 patients collected at 3-year intervals from 1990 to 1996. GBV-C/HGV RNA was determined by reverse transcriptase-polymerase chain reaction and antibodies to E2-protein by an enzyme immunoassay. At baseline, 14.5% of patients had viremia and 18.5% anti-E2. None of the patients with anti-E2 in 1990 subsequently became viremic. Of the 100 GBV-C/HGV RNA−, anti-E2− patients, 10 acquired infection during follow-up, as indicated by positivity of GBV-C/HGV RNA (n = 2), anti-E2 (n = 7), or both markers (n = 1) in 1996. The incidence was 1.7 per 100 person-years (95% confidence interval [CI], 0.8 to 3). Since approximately 19,000 blood units were transfused to these patients during follow-up, the risk of infection was 5.3 in 10,000 units (95% CI, 2 to 8.5). Six of 22 viremic patients cleared the virus during follow-up; 4 of them became anti-E2+. Twelve of 28 patients lost anti-E2 reactivity during follow-up. In conclusion, more than 25% of infections resolve within 6 years; the presence of anti-E2 seems to be protective against infection. Anti-E2 reactivity may decrease with time.

scholarly journals The Incidence and Natural Course of Transfusion-Associated GB Virus C/Hepatitis G Virus Infection in a Cohort of Thalassemic Patients

Blood ◽  
1998 ◽  
Vol 91 (3) ◽  
pp. 774-777 ◽  
Author(s):  
Daniele Prati ◽  
Alberto Zanella ◽  
Patrizia Bosoni ◽  
Paolo Rebulla ◽  
Elena Farma ◽  
...  
Keyword(s):  
Natural Course ◽  
Chain Reaction ◽  
Serum Samples ◽  
Hepatitis G Virus ◽  
Gb Virus C ◽  
Polymerase Chain ◽  
Hepatitis G ◽  
Virus C ◽  
A Prospective Study

Abstract To evaluate the risk of transmitting blood-borne GB virus C/hepatitis G virus (GBV-C/HGV) and to define the natural course of infection, we performed a prospective study in a cohort of multitransfused β-thalassemics during a 6-year follow-up period. We analyzed serum samples of 150 patients collected at 3-year intervals from 1990 to 1996. GBV-C/HGV RNA was determined by reverse transcriptase-polymerase chain reaction and antibodies to E2-protein by an enzyme immunoassay. At baseline, 14.5% of patients had viremia and 18.5% anti-E2. None of the patients with anti-E2 in 1990 subsequently became viremic. Of the 100 GBV-C/HGV RNA−, anti-E2− patients, 10 acquired infection during follow-up, as indicated by positivity of GBV-C/HGV RNA (n = 2), anti-E2 (n = 7), or both markers (n = 1) in 1996. The incidence was 1.7 per 100 person-years (95% confidence interval [CI], 0.8 to 3). Since approximately 19,000 blood units were transfused to these patients during follow-up, the risk of infection was 5.3 in 10,000 units (95% CI, 2 to 8.5). Six of 22 viremic patients cleared the virus during follow-up; 4 of them became anti-E2+. Twelve of 28 patients lost anti-E2 reactivity during follow-up. In conclusion, more than 25% of infections resolve within 6 years; the presence of anti-E2 seems to be protective against infection. Anti-E2 reactivity may decrease with time.

Detection of GB Virus-C/Hepatitis G Virus RNA in Serum by Reverse Transcription Polymerase Chain Reaction

1997 ◽  
Vol 52 (1) ◽  
pp. 92-96 ◽  
Author(s):  
Bhavna Bhardwaj ◽  
KePing Qian ◽  
Jill Detmer ◽  
Masashi Mizokami ◽  
Janice A. Kolberg ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Reverse Transcription ◽  
Chain Reaction ◽  
Hepatitis G Virus ◽  
Gb Virus C ◽  
Virus Rna ◽  
Polymerase Chain ◽  
Hepatitis G ◽  
Virus C

GB Virus C/Hepatitis G Virus Isolates in Japanese Haemophiliacs and their Origins

1998 ◽  
Vol 80 (08) ◽  
pp. 242-245 ◽  
Author(s):  
Yoshihide Fukuda ◽  
Tetsuo Hayakawa ◽  
Junki Takamatsu ◽  
Hidehiko Saito ◽  
Hiroaki Okamoto ◽  
...  
Keyword(s):  
Sequence Similarity ◽  
West African ◽  
Blood Products ◽  
Hepatitis G Virus ◽  
Gb Virus C ◽  
Route Of Transmission ◽  
Polymerase Chain ◽  
Hepatitis G ◽  
Virus C ◽  
Virus Isolates

SummaryJapanese haemophiliacs have been at high risk for infection with parenterally-transmissible viruses through the use of blood products, especially imported ones. Recently, novel transfusion-transmissible virus, GB virus C (GBV-C)/hepatitis G virus (HGV) were isolated. We investigated the origin and route of transmission of GBV-C/HGV isolates in haemophiliacs in Japan. GBV-C/HGV RNA was measured by nested reverse transcription polymerase chain reaction in 91 Japanese haemophiliacs. Phylogenetic analysis and genotypic grouping of GBV-C/HGV isolates in Japanese haemophiliacs were performed based on sequences in the 5’ untranslated region, and the characteristics were compared with those of reported isolates. GBV-C/HGV infection was present in 19 of 91 haemophiliacs (20.9%). Sequence analysis showed that 15 of the 19 isolates (78.9%) showed sequence similarity to a group in which mainly West African isolates have been reported. The other 4 isolates (21.1%) showed sequence similarity to Asian isolates. None of the GBV-C/HGV isolates showed sequences similar to those generally found in isolates from USA and Europe. The majority of GBV-C/HGV isolates found in Japanese haemophiliacs who are considered to have been infected by imported blood products were similar to those detected in West Africa.

Detection of active hepatitis C virus and hepatitis G virus/GB virus C replication in bone marrow in human subjects

Blood ◽  
2000 ◽  
Vol 95 (12) ◽  
pp. 3986-3989 ◽  
Author(s):  
Marek Radkowski ◽  
Joanna Kubicka ◽  
Elzbieta Kisiel ◽  
Janusz Cianciara ◽  
Marek Nowicki ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Human Subjects ◽  
Hcv Rna ◽  
Serum Samples ◽  
Hepatitis G Virus ◽  
Polymerase Chain ◽  
Hepatitis G ◽  
Virus C

Abstract We have analyzed the presence of hepatitis C virus (HCV) and hepatitis G virus (HGV) sequences in bone marrow and serum samples from 48 patients of a hematologic outpatient clinic. HCV RNA was detected in 18 (38%) and 15 (31%) and HGV RNA was detected in 6 (13%) and 9 (19%) of serum and bone marrow samples, respectively. In 3 patients, HGV RNA was detectable in bone marrow but not in the serum; 2 of these patients were negative for the presence of specific antibodies. Using a highly strand-specific Tth-based reverse transcriptase-polymerase chain reaction (RT-PCR), the presence of HCV RNA and HGV RNA negative strand was demonstrated in 4 and 5 bone marrow samples, respectively. Our study shows that HCV and HGV can replicate in bone marrow; in the case of HGV, analysis of serum may underestimate the true prevalence of infection.

Detection of active hepatitis C virus and hepatitis G virus/GB virus C replication in bone marrow in human subjects

Blood ◽  
2000 ◽  
Vol 95 (12) ◽  
pp. 3986-3989 ◽  
Author(s):  
Marek Radkowski ◽  
Joanna Kubicka ◽  
Elzbieta Kisiel ◽  
Janusz Cianciara ◽  
Marek Nowicki ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Human Subjects ◽  
Hcv Rna ◽  
Serum Samples ◽  
Hepatitis G Virus ◽  
Polymerase Chain ◽  
Hepatitis G ◽  
Virus C

We have analyzed the presence of hepatitis C virus (HCV) and hepatitis G virus (HGV) sequences in bone marrow and serum samples from 48 patients of a hematologic outpatient clinic. HCV RNA was detected in 18 (38%) and 15 (31%) and HGV RNA was detected in 6 (13%) and 9 (19%) of serum and bone marrow samples, respectively. In 3 patients, HGV RNA was detectable in bone marrow but not in the serum; 2 of these patients were negative for the presence of specific antibodies. Using a highly strand-specific Tth-based reverse transcriptase-polymerase chain reaction (RT-PCR), the presence of HCV RNA and HGV RNA negative strand was demonstrated in 4 and 5 bone marrow samples, respectively. Our study shows that HCV and HGV can replicate in bone marrow; in the case of HGV, analysis of serum may underestimate the true prevalence of infection.

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