Monitoring of ionic content of exhaled breath condensate in various respiratory diseases by capillary electrophoresis with contactless conductivity detection

There is increasing evidence that biomarkers of exhaled gases or exhaled breath condensate (EBC) may help in detecting abnormalities in respiratory diseases mirroring increased, oxidative stress, airways inflammation and endothelial dysfunction. Beside the traditional techniques to investigate biomarker profiles, “omics” sciences have raised interest in the clinical field as potentially improving disease phenotyping. In particular, metabonomics appears to be an important tool to gain qualitative and quantitative information on low-molecular weight metabolites present in cells, tissues, and fluids. Here, we review the potential use of EBC as a suitable matrix for metabonomic studies using nuclear magnetic resonance (NMR) spectroscopy. By using this approach in airway diseases, it is now possible to separate specific EBC profiles, with implication in disease phenotyping and personalized therapy.

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Determination of phenytoin in exhaled breath condensate using electromembrane extraction followed by capillary electrophoresis

Electrophoresis ◽

10.1002/elps.201900440 ◽

2020 ◽

Vol 41 (9) ◽

pp. 666-677 ◽

Cited By ~ 1

Author(s):

Behrouz Seyfinejad ◽

Ali Meshkini ◽

Paria Habibolahi ◽

Sibel A. Ozkan ◽

Abolghasem Jouyban

Keyword(s):

Capillary Electrophoresis ◽

Exhaled Breath Condensate ◽

Exhaled Breath ◽

Electromembrane Extraction ◽

Breath Condensate

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Study of Various Parameters that Influence the Content of Exhaled Breath Condensate Used in the Diagnosis of Gastroesophageal Reflux Disease

Hungarian Journal of Industry and Chemistry ◽

10.1515/hjic-2018-0007 ◽

2018 ◽

Vol 46 (1) ◽

pp. 29-34 ◽

Cited By ~ 3

Author(s):

Michal Greguš ◽

Pavlo Ďurč ◽

Julia Lačná ◽

František Foreti ◽

Peter Kubáň

Keyword(s):

Gastroesophageal Reflux Disease ◽

Gastroesophageal Reflux ◽

Reflux Disease ◽

Exhaled Breath Condensate ◽

Background Electrolyte ◽

Exhaled Breath ◽

Sampling Device ◽

Rapid Separation ◽

Ionic Content ◽

Breath Condensate

Abstract In this work, various parameters that influence the ionic content and pH of exhaled breath condensate in of the noninvasive diagnosis of gastroesophageal reflux disease were studied. Exhaled breath condensate samples were collected using a miniature and inexpensive sampling device. Capillary electrophoresis with contactless conductometric detection was used to monitor the ionic content of exhaled breath condensate. Background electrolyte composed of 20 mM of 2-(N-Morpholino)ethanesulfonic acid, 20 mM of L-Histidine, 2 mM of 18-Crown-6 and 30 M of cetyltrimethylammonium bromide facilitated the rapid separation of anions and cations, both in less than 2 minutes. The possibility of contamination of the exhaled breath condensate by saliva is discussed in detail. The day-to-day repeatability (n=5) of the ionic content and pH of the exhaled breath condensate was studied and was satisfactory, reflecting mainly the physiological variability

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