scholarly journals Effect of the expression and knockdown of citrate synthase gene on carbon flux during triacylglycerol biosynthesis by green algae Chlamydomonas reinhardtii

2013 ◽  
Vol 14 (1) ◽  
pp. 38 ◽  
Author(s):  
Xiaodong Deng ◽  
Jiajia Cai ◽  
Xiaowen Fei
1993 ◽  
Vol 48 (1-2) ◽  
pp. 41-45 ◽  
Author(s):  
Thomas Urbig ◽  
Rüdiger Schulz ◽  
Horst Senger

The hydrogenases of the green algae Scenedesmus obliquus and Chlamydomonas reinhardtii were activated under anaerobic conditions. Exposure of whole cells and cell-free homogenates to air lead to a complete inactivation of the hydrogenases. The inactivation in whole cells of Scenedesmus is faster than the inactivation of the cell-free homogenate. Inactivation of the hydrogenases could be reversed by anaerobic readaptation in whole cells. The inactivation of the hydrogenase in homogenates seems to be irreversible. Neither the removal of oxygen nor the addition of ATP, NAD(P)H, sodium dithionite, dithiothreitol, ferredoxin and thioredoxin to homogenates facilitated the reactivation of the hydrogenase. The occurrence of a hydrogenase regulating factor is discussed.


Author(s):  
Thomas E. Murphy ◽  
Halil Berberog˘lu

This paper reports the cellular photosynthetic rates of the green algae Chlamydomonas reinhardtii wild strain and its truncated chlorophyll antenna transformant, tla1, as a function of local irradiance. It is hypothesized that reduction in the pigmentation of algae cells can enhance light peneration in mass cultures and increase productivity. Thus, an experimental setup was designed to expose each cell within planktonic algae cultures to a nearly uniform irradiance. An oxygen microsensor was used to monitor the photosynthetic rate as the irradiance onto the sample was varied. The results showed that the cellular photosynthetic rate of the wild strain, CC125, was greater than that of tla1 at all irradiances, by a factor that ranged from 1.7 to 4. Photoinhibition was observed in both strains, although the effect was more pronounced in CC125. Although less pigmented cells enable deeper light penetration in photobioreactors, their reduced phosotynthetic rate can negate this benefit.


2002 ◽  
Vol 25 (1) ◽  
pp. 39-45 ◽  
Author(s):  
Hitoshi TANAKA ◽  
Ju-Hyun KIM ◽  
Shogo NAKAMURA ◽  
Ryuichi SUDO

2007 ◽  
Vol 65 (7-8) ◽  
pp. 447-451 ◽  
Author(s):  
Xiujuan Liu ◽  
Xuedong Wang ◽  
Xiaowen Chen ◽  
Shao Yang

2015 ◽  
Vol 5 (7) ◽  
pp. 589-596 ◽  
Author(s):  
Javier A. Gimpel ◽  
Hussam H. Nour-Eldin ◽  
Melissa A. Scranton ◽  
Daphne Li ◽  
Stephen P. Mayfield

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