Refactoring the Six-Gene Photosystem II Core in the Chloroplast of the Green Algae Chlamydomonas reinhardtii

2015 ◽  
Vol 5 (7) ◽  
pp. 589-596 ◽  
Author(s):  
Javier A. Gimpel ◽  
Hussam H. Nour-Eldin ◽  
Melissa A. Scranton ◽  
Daphne Li ◽  
Stephen P. Mayfield
Keyword(s):  
Photosystem Ii ◽  
Chlamydomonas Reinhardtii ◽  
Green Algae

Inactivation and Reactivation of the Hydrogenases of the Green Algae Scenedesmus obliquus and Chlamydomonas reinhardtii

1993 ◽  
Vol 48 (1-2) ◽  
pp. 41-45 ◽  
Author(s):  
Thomas Urbig ◽  
Rüdiger Schulz ◽  
Horst Senger
Keyword(s):  
Chlamydomonas Reinhardtii ◽  
Green Algae ◽  
Scenedesmus Obliquus ◽  
Sodium Dithionite ◽  
Anaerobic Conditions ◽  
Whole Cells ◽  
Complete Inactivation

The hydrogenases of the green algae Scenedesmus obliquus and Chlamydomonas reinhardtii were activated under anaerobic conditions. Exposure of whole cells and cell-free homogenates to air lead to a complete inactivation of the hydrogenases. The inactivation in whole cells of Scenedesmus is faster than the inactivation of the cell-free homogenate. Inactivation of the hydrogenases could be reversed by anaerobic readaptation in whole cells. The inactivation of the hydrogenase in homogenates seems to be irreversible. Neither the removal of oxygen nor the addition of ATP, NAD(P)H, sodium dithionite, dithiothreitol, ferredoxin and thioredoxin to homogenates facilitated the reactivation of the hydrogenase. The occurrence of a hydrogenase regulating factor is discussed.

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