The relationships between donor age and gender and initial isolation yield and the
osteogenic potentials of human bone marrow stromal cells (hBMSCs) have not been clearly
elucidated. The authors investigated whether isolation yields and the osteogenic differentiation
potentials of hBMSCs are indeed dependent on donor age or gender. Fresh bone marrow was
aspirated from iliac crest of 72 donors (mean age 54.1 years; range, 23-84 years; 39 men and 33
women) undergoing total hip arthroplasty. Numbers of mononuclear cells, numbers of colony
forming unit-fibroblasts (CFU-Fs) and alkaline phosphatase (ALP)-positive CFU-Fs, and numbers
of BMSCs after isolation culture were not found to be significantly dependent on donor age or
gender. Moreover, no significant age- or gender-related differences were observed in terms of the
proliferation activities, ALP activities, and calcium contents of BMSCs during in vitro osteogenic
differentiation. The data obtained from 72 human donors revealed no significant age- or genderrelated
differences among hBMSCs in terms of isolation yields, proliferation activities, and
osteogenic potentials.