scholarly journals Directional responses following recombinant cytokine stimulation of rainbow trout (Oncorhynchus mykiss) RTS-11 macrophage cells as revealed by transcriptome profiling

BMC Genomics ◽  
2007 ◽  
Vol 8 (1) ◽  
pp. 150 ◽  
Author(s):  
Samuel AM Martin ◽  
Jun Zou ◽  
Dominic F Houlihan ◽  
Christopher J Secombes
Keyword(s):  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Transcriptome Profiling ◽  
Macrophage Cells ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Recombinant Cytokine ◽  
Cytokine Stimulation ◽  
Stimulation Of

Transcriptome profiling of farmed rainbow trout (Oncorhynchus mykiss) liver from different sources of dietary zinc

Aquaculture ◽  
2021 ◽  
pp. 737017
Author(s):  
Sajad Nazari ◽  
Mohammad Pourkazemi ◽  
Hamed Paknejad ◽  
Esmaeel Kazemi ◽  
Mostafa Ghaderi ◽  
...  
Keyword(s):  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Transcriptome Profiling ◽  
Dietary Zinc ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Different Sources

Insulin and insulin-like growth factor I signaling pathways in rainbow trout (Oncorhynchus mykiss) during adipogenesis and their implication in glucose uptake

2010 ◽  
Vol 299 (1) ◽  
pp. R33-R41 ◽  
Author(s):  
L. Bouraoui ◽  
E. Capilla ◽  
J. Gutiérrez ◽  
I. Navarro
Keyword(s):  
Rainbow Trout ◽  
Growth Factor ◽  
Oncorhynchus Mykiss ◽  
Glucose Uptake ◽  
Signaling Pathways ◽  
Mapk Phosphorylation ◽  
Factor I ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Igf I ◽  
Stimulation Of

Primary cultures of rainbow trout ( Oncorhynchus mykiss ) adipocytes were used to examine the main signaling pathways of insulin and insulin-like growth factor I (IGF-I) during adipogenesis. We first determined the presence of IGF-I receptors (IGF-IR) and insulin receptors (IR) in trout preadipocytes ( day 5) and adipocytes ( day 14). IGF-IRs were more abundant and appeared to be in higher levels in differentiated cells than in preadipocytes, whereas IRs were detected in lower but constant levels throughout the culture. The cells were immunoreactive against ERK1/2 MAPK, and AKT/PI3K, components of the two main signal transduction pathways for insulin and IGF-I receptors. Stimulation of MAPK phosphorylation by IGF-I was higher in preadipocytes than in adipocytes, while no effects were observed in MAPK phosphorylation after incubation of cells with insulin. AKT phosphorylation increased in the presence of both insulin and IGF-I, with higher levels of stimulation in adipocytes than in preadipocytes. Activation of both pathways was blocked by the use of specific inhibitors of MAPK (PD98059) and AKT (wortmannin). We describe here, for the first time, the effects of IGF-I and insulin on 2-deoxyglucose uptake in primary culture of trout adipocytes. IGF-I was more potent in stimulating glucose uptake than insulin, and PD98059 and wortmannin inhibited the stimulation of glucose uptake by this growth factor, suggesting that IGF-I plays an important metabolic role in trout adipocytes. Our results suggest that differential activation of the MAPK and AKT pathways are involved in the IGF-I- and insulin-induced effects of trout adipocytes during the various stages of adipogenesis.

Consequences of Pulsed DC Electrofishing and Air Exposure to Rainbow Trout (Oncorhynchus mykiss)

1994 ◽  
Vol 51 (8) ◽  
pp. 1791-1798 ◽  
Author(s):  
C. J. A. Mitton ◽  
D. G. McDonald
Keyword(s):  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Additional Stress ◽  
Physical Damage ◽  
Early Recovery ◽  
Air Exposure ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Pulsed Dc ◽  
Fish Collection ◽  
Stimulation Of

Electrofishing, a technique widely used for fish collection, is a procedure that is often followed by handling and air exposure before fish are released. In this study the pathological and physiological consequences of capture with pulsed DC (pDC) electroshock with and without the additional disturbance of air exposure were examined on rainbow trout (Oncorhynchus mykiss). Electroshocked fish showed no evidence of direct mortality, and skeletal damage was only induced under the most severe conditions (~600-g fish, 600 V, 20 or 40 s). Physiological disturbances induced by pDC, in the absence of physical damage, consisted of lactacidosis and stimulation of the interrenal stress response. The acidosis was likely induced by tetany during immobilization and apnea during immobilization and early recovery. In fact, the physiological disturbances induced by pDC were similar in magnitude and duration to 2–3 min of exhaustive exercise and generally more severe than responses previously reported for nonpulsed (i.e., continous) DC gear. In addition, following pDC, fish appeared to experience a mild narcosis, as was evident by a reduced response to the additional stress of air exposure.

Adrenergic stimulation of glycolysis without change in glycolytic enzyme binding in rainbow trout (Oncorhynchus mykiss) erythrocytes

1994 ◽  
Vol 72 (5) ◽  
pp. 950-953 ◽  
Author(s):  
Dawn H. Sephton ◽  
William R. Driedzic
Keyword(s):  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Whole Blood ◽  
Glycolytic Enzyme ◽  
Adrenergic Stimulation ◽  
Enzyme Binding ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Cell Membrane Binding ◽  
Stimulation Of

Whole blood from rainbow trout (Oncorhynchus mykiss) was incubated in vitro with pharmacological levels of isoproterenol. The adrenergic stimulation of glycolysis in erythrocytes (RBCs) was assessed by monitoring the rate of 14C incorporation from [6-14C]-glucose into the acid-soluble RBC fraction. During a 3-h in vitro incubation, incorporation of label into the acid-soluble RBC fraction of isoproterenol-treated whole blood (0.25 ± 0.04 μmol glucose∙mL−1 RBC∙h−1) was higher than into untreated blood (0.08 ± 0.01 μmol glucose∙mL−1 RBC∙h−1). The percentage of cell membrane binding for phosphofructokinase, aldolase, and glyceraldehyde-3-phosphate dehydrogenase ranged from 17 to 35% and was not altered by adrenergic stimulation. Adrenergic stimulation activates glycolysis in rainbow trout RBCs but not through the modulation of enzyme binding.

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