Effects of Systematic Influences and Intramammary Infection on Differential and Total Somatic Cell Counts in Quarter Milk Samples from Dairy Cows

Pooled milk samples from 115 Bergamasca meat sheep were collected aseptically five times from lambing to weaning to determine the prevalence of intramammary infection, somatic cell counts and milk quality parameters (protein, fat and lactose), and effects of infection on lamb weight gain. The global prevalence of subclinical intramammary infection was 51·2%. The Staphylococcus genus was responsible for the greatest prevalence (53·3% among infected udders). Staphylococcus aureus was isolated in 8·4% of infected milk samples. Infection status had significant effects on fat and protein percentage and on somatic cell count. Lamb growth was greatest for lambs of ewes with no infection and decreased as the number of infected samples increased. No significant differences were detected in the growth of lambs with dams infected by different bacterial species.

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Frequency of individual udder quarters with elevated CMT scores in cows' milk samples with low somatic cell counts

Veterinary Record ◽

10.1136/vr.155.7.213 ◽

2004 ◽

Vol 155 (7) ◽

pp. 213-213 ◽

Cited By ~ 5

Author(s):

I. Berglund ◽

G. Pettersson ◽

K. Svennersten‐Sjaunja ◽

K. Östensson

Keyword(s):

Somatic Cell ◽

Somatic Cell Counts ◽

Milk Samples ◽

Cell Counts

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Carryover of aflatoxin from feed to milk in dairy cows with low or high somatic cell counts

animal ◽

10.1017/s1751731107000663 ◽

2007 ◽

Vol 1 (9) ◽

pp. 1344-1350 ◽

Cited By ~ 70

Author(s):

F. Masoero ◽

A. Gallo ◽

M. Moschini ◽

G. Piva ◽

D. Diaz

Keyword(s):

Dairy Cows ◽

Somatic Cell ◽

Somatic Cell Counts ◽

Cell Counts

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Rapid Detection of Mycoplasma bovis, Staphylococcus aureus and Streptococcus agalactiae in Cattle Bulk Tank Milk in Cyprus and Relations with Somatic Cell Counts

Pathogens ◽

10.3390/pathogens10070841 ◽

2021 ◽

Vol 10 (7) ◽

pp. 841

Author(s):

Maria Liapi ◽

George Botsaris ◽

Costas Arsenoglou ◽

Nikolas Markantonis ◽

Christodoulos Michael ◽

...

Keyword(s):

Staphylococcus Aureus ◽

Somatic Cell ◽

Real Time ◽

Streptococcus Agalactiae ◽

Mycoplasma Bovis ◽

Bulk Tank Milk ◽

Somatic Cell Counts ◽

Milk Samples ◽

Cell Counts ◽

Bulk Tank

One hundred and seventy-seven (177) bulk tank milk samples were analyzed with a commercially available real-time polymerase chain reaction kit and 11 (6.21%), 41 (23.16%), and 58 (32.77%) tested positive for Mycoplasma bovis, Staphylococcus aureus, and Streptococcus agalactiae, respectively. Statistical analysis revealed a significant relationship between the presence of S. aureus and S. agalactiae. Enumeration of somatic cells was performed in the same samples by flow cytometry. The somatic cell counts were found higher in S. aureus and S. agalactiae positive samples. No association was found between M. bovis presence and somatic cells counts. Low internal assay control Ct values were found to be related with high somatic cell counts. Noticeably, this is the first report for the presence of M. bovis in Cyprus. Therefore, its presence was confirmed by bulk tank milk culture, conventional PCR, and next generation sequencing. Furthermore, M. bovis was typed with multilocus sequencing typing and was allocated to sequence type 29 (ST 29). Real-time PCR in bulk tank milk samples is a useful tool to detect mammary infections, especially for neglected pathogens such as M. bovis.

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