Correction to: Homology analysis between clinically isolated extraintestinal and enteral Klebsiella pneumoniae among neonates

ABSTRACTKlebsiella Pneumoniae is a gram-negative bacterium that is known for causing infection in nosocomial settings. As reported by WHO, this bacterial pathogen is classified as an urgent threat our most concern is that these bacterial pathogens acquired genetic traits that make them resistant towards antibiotics. The last class of antibiotics; carbapenems are not able to combat these bacterial pathogens allowing them to clonally expand their antibiotic-resistant strain. Most antibiotics target the essential pathways of the bacteria cell however these targets are no longer susceptible to the antibiotic. Hence in our study, we focus on Klebsiella Pneumoniae bacterial strains that contain DNA Adenine Methyltransferase domain which suggests a new potential site for a drug target. DNA methylation is seen to regulate the attenuation of bacterial virulence. In this study, all hypothetical proteins of Klebsiella Pneumoniae containing N6 DNA Adenine Methyltransferase domain were analysed for a potential drug target. About 32 hypothetical proteins were retrieved from Uniprot. 19 proteins were selected through a step-wise subtractive genomics approach like a selection of non-homologus proteins against the human host, selection of bacterial proteins contains an essential gene, broad-spectrum analysis, druggability analysis, Non-homology analysis against gut microbiota. Through drug target prioritization like sub-cellular analysis, drug property analysis, anti-target non-homology analysis, virulence factor analysis and protein-protein interaction analysis one drug target protein (Uniprot ID: A0A2U0NNR3) was prioritized. Identified drug target docked with potential inhibitors like are mahanine (PubChem ID: 375151), curcumin (PubChem ID: 969516), EGCG (PubChem ID: 65064), nanaomycin A (PubChem ID: 40586), parthenolide (PubChem ID: 7251185), quercetin (PubChem ID: 5280343) and trimethylaurintricarboxylic acid. Based on the moelcular docking analysis, mahanine has the highest binding affinity. In order to identify novel natural inhibitor based on mahanine fingerprint search is performed against NPASS (Natural Product Activity and Species Source databases) and Koenimbine was identified as a novel natural inhibitor based on virtual screening.

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Homology analysis between clinically isolated extraintestinal and enteral Klebsiella pneumoniae among Neonates

10.21203/rs.3.rs-29301/v2 ◽

2020 ◽

Author(s):

Chun-mei Chen ◽

Min Wang ◽

Xian-ping Li ◽

Peng-ling Li ◽

Jing-jing Tian ◽

...

Keyword(s):

Antibiotic Resistance ◽

Klebsiella Pneumoniae ◽

Carbapenem Resistance ◽

Gi Tract ◽

Homology Analysis ◽

Gastrointestinal Colonization ◽

Sequence Types

Abstract Background: Klebsiella pneumoniae is a leading cause of hospital-associated (HA) infections . It has been reported that gastrointestinal colonization (GI) is likely to be a common and significant reservoir for the transmission and infections of K. pneumoniae in both adults and neonates. However, the homologous relationship between clinically isolated extraintestinal and enteral K. pneumoniae in neonates hasn’t been characterized yet. Results: 43 isolates from 21 neonatal patients were collected in this study. The proportion of carbapenem resistance was 62.8%. There were 12 patients (12/21, 57.4%) whose antibiotic resistance phenotypes, genotypes, and ST types (STs) were concordant. Six sequence types were detected using MLST, with ST37 and ST54 being the dominant types. The results of MLST were consist with the results of PFGE. Conclusions: These data showed that there might be a close homologous relationship between EXKP and EKP in neonates，indicating that the K. pneumoniae from the GI tract is possibly to be a significant reservoir for causing extraintestinal infections.

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Homology analysis between clinically isolated extraintestinal and enteral Klebsiella pneumoniae among Neonates

10.21203/rs.3.rs-29301/v1 ◽

2020 ◽

Author(s):

Chun-mei Chen ◽

Min Wang ◽

Xian-ping Li ◽

Peng-ling Li ◽

Jing-jing Tian ◽

...

Keyword(s):

Antibiotic Resistance ◽

Klebsiella Pneumoniae ◽

Carbapenem Resistance ◽

Gi Tract ◽

Homology Analysis ◽

Gastrointestinal Colonization ◽

Sequence Types

Abstract Background: Klebsiella pneumoniae is a leading cause of hospital-associated (HA) infections. It has been reported in many studies that gastrointestinal colonization (GI) is likely to be a common and significant reservoir for the transmission and infections of K. pneumoniae in both adults and neonates. But the homologous relationship between clinically isolated extraintestinal and enteral K. pneumoniae in neonates hasn’t been characterized yet.Results: 43 isolates from 21 neonatal patients were collected in this study. The proportion of carbapenem resistance was 62.8%. There were 12 patients (12/21, 57.4%) whose antibiotic resistance phenotypes, genotypes and ST types (STs) were concordant. Six sequence types were detected using MLST, with ST37 and ST54 being the dominant types. Conclusions: These data showed that there might be a close homologous relationship between EXKP and EKP in neonates，indicating that the K. pneumoniae from the GI tract is possibly to be a significant reservoir for causing extraintestinal infections.

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Homology analysis between clinically isolated extraintestinal and enteral Klebsiella pneumoniae among Neonates

10.21203/rs.3.rs-29301/v3 ◽

2020 ◽

Author(s):

Chun-mei Chen ◽

Min Wang ◽

Xian-ping Li ◽

Peng-ling Li ◽

Jing-jing Tian ◽

...

Keyword(s):

Antibiotic Resistance ◽

Klebsiella Pneumoniae ◽

Carbapenem Resistance ◽

Gi Tract ◽

Homology Analysis ◽

Gastrointestinal Colonization ◽

Sequence Types

Abstract Background: Klebsiella pneumoniae is a leading cause of hospital-associated (HA) infections. It has been reported that gastrointestinal colonization (GI) is likely to be a common and significant reservoir for the transmission and infections of K. pneumoniae in both adults and neonates. However, the homologous relationship between clinically isolated extraintestinal and enteral K. pneumoniae in neonates hasn’t been characterized yet.Results: 43 isolates from 21 neonatal patients were collected in this study. The proportion of carbapenem resistance was 62.8%. There were 12 patients (12/21, 57.4%) whose antibiotic resistance phenotypes, genotypes, and ST types (STs) were concordant. Six sequence types were detected using MLST, with ST37 and ST54 being the dominant types. The results of MLST were consist with the results of PFGE.Conclusions: These data showed that there might be a close homologous relationship between extraintestinal K. pneumoniae (EXKP) and enteral K. pneumoniae (EKP) in neonates，indicating that the K. pneumoniae from the GI tract is possibly to be a significant reservoir for causing extraintestinal infections.

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The structure of the core region of the lipopolysaccharide from Klebsiella pneumoniae O3 3-Deoxy-alpha-D-manno-octulosonic acid (alpha-Kdo) residue in the outer part of the core, a common structural element of Klebsiella pneumoniae O1, O2, O3, O4, O5, O8, and O12 lipopolysaccharides

European Journal of Biochemistry ◽

10.1046/j.1432-1033.2001.02047.x ◽

2001 ◽

Vol 268 (6) ◽

pp. 1722-1729 ◽

Cited By ~ 3

Author(s):

Evgeny Vinogradov ◽

Maciej Cedzynski ◽

Andrzej Ziolkowski ◽

Anna Swierzko

Keyword(s):

Klebsiella Pneumoniae ◽

Outer Part ◽

Core Region ◽

Structural Element ◽

The Core

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Is the antigenic nonapeptide of the HSP60 of Klebsiella pneumoniae the triggering factor in ankylosing spondylitis?

Immunology Letters ◽

10.1016/s0165-2478(97)88073-x ◽

1997 ◽

Vol 56 (1-3) ◽

pp. 306

Author(s):

E García-Latorre

Keyword(s):

Ankylosing Spondylitis ◽

Klebsiella Pneumoniae ◽

Triggering Factor

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Combination of Antibiotic Treatment with the Thrombin Inhibitor Recombinant Hirudin for the Therapy of Experimental Klebsiella pneumoniae Sepsis

Thrombosis and Haemostasis ◽

10.1055/s-0038-1642520 ◽

1994 ◽

Vol 71 (06) ◽

pp. 768-772 ◽

Cited By ~ 14

Author(s):

Gerhard Dickneite ◽

Jörg Czech

Keyword(s):

Septic Shock ◽

Klebsiella Pneumoniae ◽

Organ Failure ◽

Therapeutic Effect ◽

Disseminated Intravascular Coagulation ◽

Post Infection ◽

Thrombin Inhibitor ◽

Bolus Administration ◽

Bacterial Burden ◽

Recombinant Hirudin

SummaryRats which were infected with the gramnegative pathogen Klebsiella pneumoniae develop disseminated intravascular coagulation (DIC), multi-organ failure (MOF) and finally die in a septic shock. We investigated the therapeutic effect of antibiotic (tobramycin) treatment combined with the infusion of the highly specific thrombin inhibitor rec. hirudin. Although administration of 2 mg/kg tobramycin alone leads to a decrease of the bacterial burden, DIC could not be prevented. Infusion of rec. hirudin (0.25 mg/kg x h) for 4 h (start of treatment 1 h post infection), in addition to a bolus administration of tobramycin, led to an amelioration of DIC parameters as fibrinogen, thrombin-antithrombin complex (TAT) and platelets. Serum transaminase levels (GOT, GPT) as a marker of MOF were significantly improved by rec. hirudin, the T50 value increased from 17 h in the tobramycin group to 42 h in the tobramycin + rec. hirudin giuup, muilality rates were 90% or 60%, respectively. Combination of heparin (10011/kg x h) and tobramycin was not effective on survival.

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