scholarly journals Molecular epidemiology and clinical characteristics of hepatitis delta virus (HDV) infected patients with elevated transaminases in Shanghai, China

2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Shanshan Wu ◽  
Yi Zhang ◽  
Yuyan Tang ◽  
Ting Yao ◽  
Mengjiao Lv ◽  
...  
Keyword(s):  
Molecular Epidemiology ◽  
Hepatitis Delta Virus ◽  
Clinical Characteristics ◽  
Hepatitis Delta ◽  
Elevated Transaminases ◽  
Delta Virus

scholarly journals Molecular epidemiology and clinical characteristics of hepatitis delta virus (HDV) infected patients with elevated transaminases in Shanghai, China

2020 ◽  
Author(s):  
Shanshan Wu ◽  
Yi Zhang ◽  
Yuyan Tang ◽  
Ting Yao ◽  
Mengjiao Lv ◽  
...  
Keyword(s):  
Next Generation Sequencing ◽  
Hepatitis Delta Virus ◽  
Clinical Characteristics ◽  
Phylogenetic Analyses ◽  
Serum Samples ◽  
Next Generation Sequencing Ngs ◽  
Elevated Transaminases ◽  
Generation Sequencing ◽  
Hdv Genotype ◽  
Delta Virus

Abstract Background: Patients coinfected with HBV and hepatitis D virus (HDV) have a greater risk of HCC and cirrhosis. The current study was undertaken to assess HDV genotype distribution and determine clinical characteristics of hepatitis delta virus (HDV) among HBsAg positive individuals in Shanghai.Method: This retrospective study involved 225 serum samples from HBsAg positive hospitalized patients from October 2010 to April 2013. HDV-specific RT-nested PCR was used to amplify HDV RNA. HDV genotypes were characterized by Next-generation sequencing (NGS), followed by phylogenetic analyses. HDV/HBV co-infected patients and HBV mono-infected patients were compared clinically and virologically.Results: Out of the 225 HBsAg-positive serum samples with elevated transaminases, HDV-RNA was identified in 11 (4.9%) HBsAg positive patients. The HBV loads in the HDV positive group were significantly lower than the HDV negative HBV-infected patients. The aminotransferase enzymes were significantly higher in HDV/HBV co-infected compared to HDV negative patients (P<0.05). Phylogenetic analyses indicated that HDV-2 genotype being the predominant genotype, other HDV genotypes were not observed. HDV/HBV patients were significantly associated with a rather unfavourable clinical outcomeConclusion： In summary, our study showed that the prevalence of HDV infection in patients with elevated transaminases is not low and the predominance of HDV genotype 2 infection in Shanghai. This finding helps us to better understand the correlation of HDV/HBV co-infection. Moreover, Next-generation sequencing (NGS) technologies provide a rapid, precise method for generating HDV genomes to define infecting genotypes.

scholarly journals Molecular epidemiology and clinical characteristics of hepatitis delta virus (HDV) infected patients with elevated transaminases in Shanghai, China

2020 ◽  
Author(s):  
Shanshan Wu ◽  
Yi Zhang ◽  
Yuyan Tang ◽  
Ting Yao ◽  
Mengjiao Lv ◽  
...  
Keyword(s):  
Next Generation Sequencing ◽  
Hepatitis Delta Virus ◽  
Clinical Characteristics ◽  
Phylogenetic Analyses ◽  
Serum Samples ◽  
Next Generation Sequencing Ngs ◽  
Elevated Transaminases ◽  
Generation Sequencing ◽  
Hdv Genotype ◽  
Delta Virus

Abstract Background: Patients coinfected with HBV and hepatitis D virus (HDV) have a greater risk of HCC and cirrhosis. The current study was undertaken to assess HDV genotype distribution and determine clinical characteristics of hepatitis delta virus (HDV) among HBsAg positive individuals in Shanghai.Method: This retrospective study involved 225 serum samples from HBsAg positive hospitalized patients from October 2010 to April 2013. HDV-specific RT-nested PCR was used to amplify HDV RNA. HDV genotypes were characterized by Next-generation sequencing (NGS), followed by phylogenetic analyses. HDV/HBV co-infected patients and HBV mono-infected patients were compared clinically and virologically.Results: Out of the 225 HBsAg-positive serum samples with elevated transaminases, HDV-RNA was identified in 11 (4.9%) HBsAg positive patients. The HBV loads in the HDV positive group were significantly lower than the HDV negative HBV-infected patients. The aminotransferase enzymes were significantly higher in HDV/HBV co-infected compared to HDV negative patients (P<0.05). Phylogenetic analyses indicated that HDV-2 genotype being the predominant genotype, other HDV genotypes were not observed. HDV/HBV patients were significantly associated with a rather unfavourable clinical outcomeConclusion： In summary, our study showed that the prevalence of HDV infection in patients with elevated transaminases is not low and the predominance of HDV genotype 2 infection in Shanghai. This finding helps us to better understand the correlation of HDV/HBV co-infection. Moreover, Next-generation sequencing (NGS) technologies provide a rapid, precise method for generating HDV genomes to define infecting genotypes.

scholarly journals Characterization of the Genotypic Profile of Hepatitis Delta Virus: Isolation of HDV Genotype-1 in the Western Amazon Region of Brazil

Intervirology ◽  
2015 ◽  
Vol 58 (3) ◽  
pp. 166-171 ◽  
Author(s):  
Luan Felipo Botelho-Souza ◽  
Deusilene Souza Vieira ◽  
Alcione de Oliveira dos Santos ◽  
André Vinycius Cunha Pereira ◽  
Juan Miguel Villalobos-Salcedo
Keyword(s):  
Hepatitis B ◽  
Hepatitis Delta Virus ◽  
Clinical Characteristics ◽  
Amazon Region ◽  
Viral Envelope ◽  
Serum Samples ◽  
Hepatitis Delta ◽  
Pcr Rflp ◽  
Hdv Infection ◽  
Delta Virus

The hepatitis delta virus (HDV) is a hepatotropic subvirus that is dependent on the hepatitis B virus (HBV) and supplies the viral envelope containing the surface antigen of hepatitis B. Viral genetic diversity is related to the geographical origin of the isolates, and there are at least eight genotypes that are referred to as HDV-1 through HDV-8. HDV-3 is responsible for epidemics of severe and fulminant hepatitis, which are common in northeastern South America. HDV-3 is prevalent in the Brazilian Amazon and is associated with the increased aggressiveness of HDV infections. Although isolated, the characteristics of the clinical presentation of HDV-1 in the Amazon region have not yet been clearly reported. Objective: This study aims to assess the genotypic and clinical characteristics of individuals with the HDV-1 genotype in the western Amazon region. Methods: The HDV was genotyped by nested PCR-RFLP and sequencing from serum samples of 56 patients with HBV/HDV infection. The genotypes were correlated with the clinical characteristics presented by patients with HBV/HDV infection. Results: A prevalence of 92.3% for the HDV-3 genotype (n = 48) and 7.6% (n = 4) for the HDV-1 genotype was observed. Conclusion: To date, this is the most extensive clinical study of HDV-1 genotype infections in the nonindigenous population of Western Amazonia.

Molecular epidemiology of hepatitis delta virus (HDV) in Iran: A preliminary report

2008 ◽  
Vol 80 (12) ◽  
pp. 2092-2099 ◽  
Author(s):  
Seyed Reza Mohebbi ◽  
Narges Zali ◽  
Faramarz Derakhshan ◽  
Ali Tahami ◽  
Reza Mashayekhi ◽  
...  
Keyword(s):  
Molecular Epidemiology ◽  
Hepatitis Delta Virus ◽  
Preliminary Report ◽  
Hepatitis Delta ◽  
Delta Virus

scholarly journals Molecular epidemiology of hepatitis delta virus among blood donors in Islamabad, Pakistan

2020 ◽  
Vol 5 (1) ◽  
pp. 96
Author(s):  
Usman Waheed ◽  
Noore Saba ◽  
Akhlaaq Wazeer ◽  
Ahmad Farooq ◽  
Muhammad Arshad ◽  
...  
Keyword(s):  
Molecular Epidemiology ◽  
Hepatitis Delta Virus ◽  
Blood Donors ◽  
Hepatitis Delta ◽  
Delta Virus

Molecular epidemiology of hepatitis delta virus in the Western Pacific region

2014 ◽  
Vol 61 (1) ◽  
pp. 34-39 ◽  
Author(s):  
Meifang Han ◽  
Margaret Littlejohn ◽  
Lilly Yuen ◽  
Rosalind Edwards ◽  
Uma Devi ◽  
...  
Keyword(s):  
Molecular Epidemiology ◽  
Hepatitis Delta Virus ◽  
Western Pacific ◽  
Pacific Region ◽  
Western Pacific Region ◽  
Hepatitis Delta ◽  
The Western Pacific ◽  
Delta Virus

scholarly journals Virological and clinical characteristics of hepatitis delta virus in South Asia

2011 ◽  
Vol 8 (1) ◽  
Author(s):  
Khalid Mumtaz ◽  
Umair S Ahmed ◽  
Sadik Memon ◽  
Ali Khawaja ◽  
Muhammad T Usmani ◽  
...  
Keyword(s):  
South Asia ◽  
Hepatitis Delta Virus ◽  
Clinical Characteristics ◽  
Hepatitis Delta ◽  
Delta Virus

scholarly journals Molecular epidemiology and clinical characteristics of hepatitis delta virus (HDV) infected patients with elevated transaminases in Shanghai, China

2020 ◽  
Author(s):  
Shanshan Wu ◽  
Yi Zhang ◽  
Yuyan Tang ◽  
Ting Yao ◽  
Mengjiao Lv ◽  
...  
Keyword(s):  
Next Generation Sequencing ◽  
Hepatitis Delta Virus ◽  
Clinical Characteristics ◽  
Phylogenetic Analyses ◽  
Serum Samples ◽  
Next Generation Sequencing Ngs ◽  
Elevated Transaminases ◽  
Generation Sequencing ◽  
Hdv Genotype ◽  
Delta Virus

Abstract Background: Patients coinfected with HBV and hepatitis D virus (HDV) have a greater risk of HCC and cirrhosis. The current study was undertaken to assess HDV genotype distribution and determine clinical characteristics of hepatitis delta virus (HDV) among HBsAg positive individuals in Shanghai.Method: This retrospective study involved 225 serum samples from HBsAg positive hospitalized patients from October 2010 to April 2013. HDV-specific RT-nested PCR was used to amplify HDV RNA. HDV genotypes were characterized by Next-generation sequencing (NGS), followed by phylogenetic analyses. HDV/HBV co-infected patients and HBV mono-infected patients were compared clinically and virologically.Results: Out of the 225 HBsAg-positive serum samples with elevated transaminases, HDV-RNA was identified in 11 (4.9%) patients. The HBV loads in the HDV positive group were significantly lower than the HDV negative HBV-infected patients. The aminotransferase enzymes were significantly higher in HDV/HBV co-infected compared to HDV negative patients (P<0.05). Phylogenetic analyses indicated that HDV-2 genotype being the predominant genotype, other HDV genotypes were not observed. HDV/HBV patients were significantly associated with a rather unfavourable clinical outcome.Conclusion： In summary, our study showed that the prevalence of HDV infection in patients with elevated transaminases is not low and the predominance of HDV genotype 2 infection in Shanghai. This finding helps us to better understand the correlation of HDV/HBV co-infection. Moreover, Next-generation sequencing (NGS) technologies provide a rapid, precise method for generating HDV genomes to define infecting genotypes.

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