scholarly journals Virus-like particles derived from Pichia pastoris-expressed dengue virus type 1 glycoprotein elicit homotypic virus-neutralizing envelope domain III-directed antibodies

2016 ◽  
Vol 16 (1) ◽  
Author(s):  
Ankur Poddar ◽  
Viswanathan Ramasamy ◽  
Rahul Shukla ◽  
Ravi Kant Rajpoot ◽  
Upasana Arora ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Dengue Virus ◽  
Virus Type ◽  
Virus Like Particles ◽  
Domain Iii

scholarly journals Crystal Structure of Dengue Virus Type 1 Envelope Protein in the Postfusion Conformation and Its Implications for Membrane Fusion

2009 ◽  
Vol 83 (9) ◽  
pp. 4338-4344 ◽  
Author(s):  
Vinod Nayak ◽  
Moshe Dessau ◽  
Kaury Kucera ◽  
Karen Anthony ◽  
Michel Ledizet ◽  
...  
Keyword(s):  
Crystal Structure ◽  
Dengue Virus ◽  
Conformational Change ◽  
Virus Type ◽  
Envelope Protein ◽  
Lipid Membrane ◽  
Ph Sensor ◽  
Endosomal Membrane ◽  
Domain Iii

ABSTRACT Dengue virus relies on a conformational change in its envelope protein, E, to fuse the viral lipid membrane with the endosomal membrane and thereby deliver the viral genome into the cytosol. We have determined the crystal structure of a soluble fragment E (sE) of dengue virus type 1 (DEN-1). The protein is in the postfusion conformation even though it was not exposed to a lipid membrane or detergent. At the domain I-domain III interface, 4 polar residues form a tight cluster that is absent in other flaviviral postfusion structures. Two of these residues, His-282 and His-317, are conserved in flaviviruses and are part of the “pH sensor” that triggers the fusogenic conformational change in E, at the reduced pH of the endosome. In the fusion loop, Phe-108 adopts a distinct conformation, forming additional trimer contacts and filling the bowl-shaped concavity observed at the tip of the DEN-2 sE trimer.

scholarly journals Antibodies Induced by Dengue Virus Type 1 and 2 Envelope Domain III Recombinant Proteins in Monkeys Neutralize Strains with Different Genotypes

2009 ◽  
Vol 16 (12) ◽  
pp. 1829-1831 ◽  
Author(s):  
Lidice Bernardo ◽  
Osmel Fleitas ◽  
Alequis Pavón ◽  
Lisset Hermida ◽  
Gerardo Guillén ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Virus Type ◽  
Recombinant Proteins ◽  
Neutralizing Antibodies ◽  
Neutralizing Capacity ◽  
Domain Iii

ABSTRACT In the present work, we evaluated the neutralizing capacity of the antibodies induced by dengue virus type 1 and 2 envelope domain III recombinant proteins in monkeys against strains of different dengue virus type 1 and 2 genotypes. Here we demonstrated that dengue virus type 1 and 2 recombinant proteins induced high titers of neutralizing antibodies against different genotype strains.

Pichia pastoris-expressed dengue virus type 2 envelope domain III elicits virus-neutralizing antibodies

2010 ◽  
Vol 167 (1) ◽  
pp. 10-16 ◽  
Author(s):  
Gaurav Batra ◽  
Rajendra Raut ◽  
Satinder Dahiya ◽  
Neha Kamran ◽  
Sathyamangalam Swaminathan ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Dengue Virus ◽  
Virus Type ◽  
Neutralizing Antibodies ◽  
Domain Iii ◽  
Dengue Virus Type 2

Optimization of conditions for secretion of dengue virus type 2 envelope domain III using Pichia pastoris

2010 ◽  
Vol 110 (4) ◽  
pp. 408-414 ◽  
Author(s):  
Gaurav Batra ◽  
Chandrasekhar Gurramkonda ◽  
Satish Kumar Nemani ◽  
Swatantra Kumar Jain ◽  
Sathyamangalam Swaminathan ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Dengue Virus ◽  
Virus Type ◽  
Domain Iii ◽  
Dengue Virus Type 2

Molecular epidemiology of dengue in the Pacific: introduction of two distinct strains of dengue virus type-1 into Hawaii

2006 ◽  
Vol 100 (4) ◽  
pp. 327-336 ◽  
Author(s):  
A. Imrie ◽  
Z. Zhao ◽  
S. N. Bennett ◽  
P. Kitsutani ◽  
M. Laille ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Molecular Epidemiology ◽  
Virus Type ◽  
The Pacific
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