scholarly journals Maize-IAS: a maize image analysis software using deep learning for high-throughput plant phenotyping

Plant Methods ◽  
2021 ◽  
Vol 17 (1) ◽  
Author(s):  
Shuo Zhou ◽  
Xiujuan Chai ◽  
Zixuan Yang ◽  
Hongwu Wang ◽  
Chenxue Yang ◽  
...  
Keyword(s):  
Computer Vision ◽  
Image Analysis ◽  
Deep Learning ◽  
High Throughput ◽  
Batch Processing ◽  
Plant Phenotyping ◽  
Plant Science ◽  
Analysis Software ◽  
Image Analysis Software ◽  
Maize Growth

Abstract Background Maize (Zea mays L.) is one of the most important food sources in the world and has been one of the main targets of plant genetics and phenotypic research for centuries. Observation and analysis of various morphological phenotypic traits during maize growth are essential for genetic and breeding study. The generally huge number of samples produce an enormous amount of high-resolution image data. While high throughput plant phenotyping platforms are increasingly used in maize breeding trials, there is a reasonable need for software tools that can automatically identify visual phenotypic features of maize plants and implement batch processing on image datasets. Results On the boundary between computer vision and plant science, we utilize advanced deep learning methods based on convolutional neural networks to empower the workflow of maize phenotyping analysis. This paper presents Maize-IAS (Maize Image Analysis Software), an integrated application supporting one-click analysis of maize phenotype, embedding multiple functions: (I) Projection, (II) Color Analysis, (III) Internode length, (IV) Height, (V) Stem Diameter and (VI) Leaves Counting. Taking the RGB image of maize as input, the software provides a user-friendly graphical interaction interface and rapid calculation of multiple important phenotypic characteristics, including leaf sheath points detection and leaves segmentation. In function Leaves Counting, the mean and standard deviation of difference between prediction and ground truth are 1.60 and 1.625. Conclusion The Maize-IAS is easy-to-use and demands neither professional knowledge of computer vision nor deep learning. All functions for batch processing are incorporated, enabling automated and labor-reduced tasks of recording, measurement and quantitative analysis of maize growth traits on a large dataset. We prove the efficiency and potential capability of our techniques and software to image-based plant research, which also demonstrates the feasibility and capability of AI technology implemented in agriculture and plant science.

scholarly journals Maize-IAS: A Maize Image Analysis Software using Deep Learning for High-throughput Plant Phenotyping

2021 ◽  
Author(s):  
Shuo Zhou ◽  
Xiujuan Chai ◽  
Zixuan Yang ◽  
Hongwu Wang ◽  
Chenxue Yang ◽  
...  
Keyword(s):  
Computer Vision ◽  
Deep Learning ◽  
High Throughput ◽  
Batch Processing ◽  
Food Sources ◽  
Plant Phenotyping ◽  
Plant Science ◽  
Phenotypic Traits ◽  
Analysis Software ◽  
Maize Growth

Abstract Background : Maize (Zea mays L.) is one of the most important food sources in the world and has been one of the main targets of plant genetics and phenotypic research for centuries. Observation and analysis of various morphological phenotypic traits during maize growth are essential for genetic and breeding study. The generally huge number of samples produce an enormous amount of high-resolution image data. While high throughput plant phenotyping platforms are increasingly used in maize breeding trials, there is a reasonable need for software tools that can automatically identify visual phenotypic features of maize plants and implement batch processing on image datasets. Results : On the boundary between computer vision and plant science, we utilize advanced deep learning methods based on convolutional neural networks to empower the workflow of maize phenotyping analysis. This paper presents Maize-PAS ( Maize Phenotyping Analysis Software), an integrated application supporting one-click analysis of maize phenotype, embedding multiple functions: I. Projection, II. Color Analysis, III. Internode length, IV. Height, V. Stem Diameter and VI. Leaves Counting. Taking the RGB image of maize as input, the software provides a user-friendly graphical interaction interface and rapid calculation of multiple important phenotypic characteristics, including leaf sheath points detection and leaves segmentation. In function Leaves Counting, the mean and standard deviation of difference between prediction and ground truth are 1.60 and 1.625. Conclusion : The Maize-PAS is easy-to-use and demands neither professional knowledge of computer vision nor deep learning. All functions for batch processing are incorporated, enabling automated and labor-reduced tasks of recording, measurement and quantitative analysis of maize growth traits on a large dataset. We prove the efficiency and potential capability of our techniques and software to image-based plant research, which also demonstrates the feasibility and capability of AI technology implemented in agriculture and plant science. Keywords : Maize phenotyping; Instance segmentation; Computer vision; Deep learning; Convolutional neural network

scholarly journals Maize-PAS: Automated Maize Phenotyping Analysis Software using Deep Learning

2020 ◽  
Author(s):  
Shuo Zhou ◽  
Xiujuan Chai ◽  
Zixuan Yang ◽  
Hongwu Wang ◽  
Chenxue Yang ◽  
...  
Keyword(s):  
Computer Vision ◽  
Deep Learning ◽  
Batch Processing ◽  
Food Sources ◽  
Plant Phenotyping ◽  
Plant Science ◽  
Phenotypic Traits ◽  
Color Analysis ◽  
Analysis Software ◽  
Maize Growth

Abstract Background: Maize (Zea mays L.) is one of the most important food sources in the world and has been one of the main targets of plant genetics and phenotypic research for centuries. Observation and analysis of various morphological phenotypic traits during maize growth are essential for genetic and breeding study. The generally huge number of samples produce an enormous amount of high-resolution image data. While high throughput plant phenotyping platforms are increasingly used in maize breeding trials, there is a reasonable need for software tools that can automatically identify visual phenotypic features of maize plants and implement batch processing on image datasets.Results: On the boundary between computer vision and plant science, we utilize advanced deep learning methods based on convolutional neural networks to empower the workflow of maize phenotyping analysis. This paper presents Maize-PAS ( Maize Phenotyping Analysis Software), an integrated application supporting one-click analysis of maize phenotype, embedding multiple functions: I. Projection, II. Color Analysis, III. Internode length, IV. Height, V. Stem Diameter and VI. Leaves Counting. Taking the RGB image of maize as input, the software provides a user-friendly graphical interaction interface and rapid calculation of multiple important phenotypic characteristics, including leaf sheath points detection and leaves segmentation. In function Leaves Counting, the mean and standard deviation of difference between prediction and ground truth are 1.60 and 1.625.Conclusion: The Maize-PAS is easy-to-use and demands neither professional knowledge of computer vision nor deep learning. All functions for batch processing are incorporated, enabling automated and labor-reduced tasks of recording, measurement and quantitative analysis of maize growth traits on a large dataset. We prove the efficiency and potential capability of our techniques and software to image-based plant research, which also demonstrates the feasibility and capability of AI technology implemented in agriculture and plant science.

scholarly journals Experimental system and image analysis software for high throughput phenotyping of mycorrhizal growth response in Brachypodium distachyon

2019 ◽  
Author(s):  
Felicia Maviane-Macia ◽  
Camille Ribeyre ◽  
Luis Buendia ◽  
Mégane Gaston ◽  
Mehdi Khafif ◽  
...  
Keyword(s):  
Image Analysis ◽  
High Throughput ◽  
Growth Response ◽  
Brachypodium Distachyon ◽  
Am Fungi ◽  
Plant Phenotyping ◽  
List Type ◽  
Analysis Software ◽  
Nitrogen And Phosphorus ◽  
Image Analysis Software

AbstractPlant growth response to Arbuscular Mycorrhizal (AM) fungi is variable and depends on genetic and environment factors that still remain largely unknown. Identification of these factors can be envisaged using high-throughput and accurate plant phenotyping.We setup experimental conditions based on a two-compartment system allowing to measure Brachypodium distachyon mycorhizal growth response (MGR) in an automated phenotyping greenhouse. We developed a new image analysis software “IPSO Phen” to estimate of B. distachyon aboveground biomass.We found a positive MGR in the B. distachyon Bd3-1 genotype inoculated with the AM fungi Rhizophagus irregularis only if nitrogen and phosphorus were added together in the compartment restricted to AM fungi. Using this condition, we found genetic diversity in B. distachyon for MGR ranging from positive to negative MGR depending on the plant genotype tested.Our result on the interaction between nitrogen and phosphorus for MGR in B. distachyon opens new perspectives about AM functioning. In addition, our open-source software allowing to test and run image analysis parameters on large amount of images generated by automated plant phenotyping facilities, will help to screen large panels of genotypes and environmental conditions to identify the factors controlling the MGR.

scholarly journals PlantCV v2: Image analysis software for high-throughput plant phenotyping

PeerJ ◽  
2017 ◽  
Vol 5 ◽  
pp. e4088 ◽  
Author(s):  
Malia A. Gehan ◽  
Noah Fahlgren ◽  
Arash Abbasi ◽  
Jeffrey C. Berry ◽  
Steven T. Callen ◽  
...  
Keyword(s):  
Image Processing ◽  
Computer Vision ◽  
Image Analysis ◽  
Image Data ◽  
Plant Phenotyping ◽  
Analysis Software ◽  
Computational Tools ◽  
Landmark Identification ◽  
Plant Image ◽  
Leaf Segmentation

Systems for collecting image data in conjunction with computer vision techniques are a powerful tool for increasing the temporal resolution at which plant phenotypes can be measured non-destructively. Computational tools that are flexible and extendable are needed to address the diversity of plant phenotyping problems. We previously described the Plant Computer Vision (PlantCV) software package, which is an image processing toolkit for plant phenotyping analysis. The goal of the PlantCV project is to develop a set of modular, reusable, and repurposable tools for plant image analysis that are open-source and community-developed. Here we present the details and rationale for major developments in the second major release of PlantCV. In addition to overall improvements in the organization of the PlantCV project, new functionality includes a set of new image processing and normalization tools, support for analyzing images that include multiple plants, leaf segmentation, landmark identification tools for morphometrics, and modules for machine learning.

scholarly journals Improved structure, function and compatibility for CellProfiler: modular high-throughput image analysis software

2011 ◽  
Vol 27 (8) ◽  
pp. 1179-1180 ◽  
Author(s):  
Lee Kamentsky ◽  
Thouis R. Jones ◽  
Adam Fraser ◽  
Mark-Anthony Bray ◽  
David J. Logan ◽  
...  
Keyword(s):  
Image Analysis ◽  
Structure Function ◽  
High Throughput ◽  
Analysis Software ◽  
Image Analysis Software

scholarly journals Investigation of Different Free Image Analysis Software for High-Throughput Droplet Detection

ACS Omega ◽  
2021 ◽  
Vol 6 (35) ◽  
pp. 22625-22634
Author(s):  
Immanuel Sanka ◽  
Simona Bartkova ◽  
Pille Pata ◽  
Olli-Pekka Smolander ◽  
Ott Scheler
Keyword(s):  
Image Analysis ◽  
High Throughput ◽  
Analysis Software ◽  
Image Analysis Software

scholarly journals PlantCV v2.0: Image analysis software for high-throughput plant phenotyping

2017 ◽  
Author(s):  
Malia A Gehan ◽  
Noah Fahlgren ◽  
Arash Abbasi ◽  
Jeffrey C Berry ◽  
Steven T Callen ◽  
...  
Keyword(s):  
Image Processing ◽  
Computer Vision ◽  
Image Analysis ◽  
Image Data ◽  
Plant Phenotyping ◽  
Analysis Software ◽  
Computational Tools ◽  
Landmark Identification ◽  
Plant Image ◽  
Leaf Segmentation

Systems for collecting image data in conjunction with computer vision techniques are a powerful tool for increasing the temporal resolution at which plant phenotypes can be measured non-destructively. Computational tools that are flexible and extendable are needed to address the diversity of plant phenotyping problems. We previously described the Plant Computer Vision (PlantCV) software package, which is an image processing toolkit for plant phenotyping analysis. The goal of the PlantCV project is to develop a set of modular, reusable, and repurposable tools for plant image analysis that are open-source and community-developed. Here we present the details and rationale for major developments in the second major release of PlantCV. In addition to overall improvements in the organization of the PlantCV project, new functionality includes a set of new image processing and normalization tools, support for analyzing images that include multiple plants, leaf segmentation, landmark identification tools for morphometrics, and modules for machine learning.

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