scholarly journals Lipophosphoglycan polymorphisms do not affect Leishmania amazonensis development in the permissive vectors Lutzomyia migonei and Lutzomyia longipalpis

2017 ◽  
Vol 10 (1) ◽  
Author(s):  
Paula M. Nogueira ◽  
Agna C. Guimarães ◽  
Rafael R. Assis ◽  
Jovana Sadlova ◽  
Jitka Myskova ◽  
...  
Keyword(s):  
Leishmania Amazonensis ◽  
Lutzomyia Longipalpis ◽  
Lutzomyia Migonei

scholarly journals Ecology of Lutzomyia longipalpis and Lutzomyia migonei in an endemic area for visceral leishmaniasis

2014 ◽  
Vol 23 (3) ◽  
pp. 320-327 ◽  
Author(s):  
Rafaella Albuquerque Silva ◽  
Fabricio Kassio Moura Santos ◽  
Lindemberg Caranha de Sousa ◽  
Elizabeth Ferreira Rangel ◽  
Claudia Maria Leal Bevilaqua
Keyword(s):  
Visceral Leishmaniasis ◽  
Leishmania Infantum ◽  
Indoor Environment ◽  
Endemic Area ◽  
Natural Infection ◽  
Sampling Site ◽  
Lutzomyia Longipalpis ◽  
Sand Flies ◽  
Sampling Points ◽  
Lutzomyia Migonei

The main vector for visceral leishmaniasis (VL) in Brazil is Lutzomyia longipalpis. However, the absence of L. longipalpis in a region of autochthonous VL demonstrates the participation of other species in the transmission of the parasite. Studies conducted in La Banda, Argentina, and São Vicente Férrer, Pernambuco State, Brazil, have correlated the absence of L. longipalpisand the presence of L. migonei with autochthonous cases of VL. In São Vicente Férrer, Pernambuco, there was evidence for the natural infection of L. migonei with Leishmania infantum chagasi. Thus, the objective of this work was to assess the ecology of the sand flies L. longipalpis and L. migonei in Fortaleza, an endemic area for VL. Insect capture was conducted at 22 sampling points distributed across four regions of Fortaleza. In total, 32,403 sand flies were captured; of these, 18,166 (56%) were identified as L. longipalpis and 14,237 (44%) as L. migonei. There were significant density differences found between the vectors at each sampling site (indoors and outdoors) (p <0.0001). These findings confirm that L. migonei and L. longipalpis are distributed throughout Fortaleza, where they have adapted to an indoor environment, and suggest that L. migonei may share the role as a vector with L. longipalpis in the transmission of VL in Fortaleza.

scholarly journals Phlebotomine sandfly species from an American visceral leishmaniasis area in the Northern Rainforest region of Pernambuco State, Brazil

2007 ◽  
Vol 23 (5) ◽  
pp. 1227-1232 ◽  
Author(s):  
Maria Rosimery de Carvalho ◽  
Bruna Santos Lima ◽  
José Ferreira Marinho-Júnior ◽  
Fernando José da Silva ◽  
Hélio França Valença ◽  
...  
Keyword(s):  
Visceral Leishmaniasis ◽  
Lutzomyia Longipalpis ◽  
Target Area ◽  
Leishmania Chagasi ◽  
Animal Shelters ◽  
Phlebotomine Sandfly ◽  
Phlebotomine Sandflies ◽  
Prevalent Species ◽  
Lutzomyia Migonei ◽  
Leishmania Parasites

The aim of this study was to identify the fauna of phlebotomine sandflies in an area with incidence of American visceral leishmaniasis (AVL) in São Vicente Férrer, a municipality (county) located in the northern Zona da Mata in Pernambuco State, Brazil. Sandfly captures were conducted monthly for four nights/month from December 2002 to November 2003, by means of manual captures (with a Shannon trap) and by CDC light traps. A total of 23,156 specimens of phlebotomines sandflies were collected in a remnant of the Atlantic rainforest, houses, and animal shelters. Lutzomyia complexa was the most prevalent species, with a frequency of 62.5% (14,445/23,156), followed by Lutzomyia migonei, with 33.2% (7,677/23,156). No Lutzomyia longipalpis (the main vector of Leishmania chagasi in Brazil and the Americas) was found in the target area. Some specimens were dissected, but no Leishmania parasites were found.

scholarly journals Assessment of PCR in the detection of Leishmania spp in experimentally infected individual phlebotomine sandflies (Diptera: Psychodidae: Phlebotominae)

2002 ◽  
Vol 44 (5) ◽  
pp. 255-259 ◽  
Author(s):  
Érika M. MICHALSKY ◽  
Consuelo L. FORTES-DIAS ◽  
Paulo F.P. PIMENTA ◽  
Nágila F.C. SECUNDINO ◽  
Edelberto S. DIAS
Keyword(s):  
Digestive Tract ◽  
Infected Individual ◽  
Dna Amplification ◽  
Leishmania Braziliensis ◽  
Lutzomyia Longipalpis ◽  
Leishmania Chagasi ◽  
Phlebotomine Sandflies ◽  
Leishmania Spp ◽  
Polymerase Chain ◽  
Lutzomyia Migonei

DNA amplification by the polymerase chain reaction (PCR) was applied in the investigation of the presence of Leishmania (Kinetoplastida: Trypanosomatidae) parasites in single phlebotomine sandflies. Three phlebotomine/parasite pairs were used: Lutzomyia longipalpis/Leishmania chagasi, Lutzomyia migonei/Leishmania amazonensis and Lutzomyia migonei/Leishmania braziliensis, all of them incriminated in the transmission of visceral or cutaneous leishmaniasis. DNA extraction was performed with whole insects, with no need of previous digestive tract dissection or pooling specimens. The presence of either mouse blood in the digestive tract of the sandflies or the digestive tract itself did not interfere in the PCR. Infection by as few as 10 Leishmania sp. per individual were sufficient for DNA amplification with genus-specific primers. Using primers for L. braziliensis and L. mexicana complexes, respectively, it was possible to discriminate between L. braziliensis and L. amazonensis in experimentally infected vectors (L. migonei).

scholarly journals Identificación rápida de Lutzomyia longipalpis a partir de cinco descriptores del aparato genital masculino

2016 ◽  
Vol 24 (1) ◽  
pp. 21
Author(s):  
E. G. Llano ◽  
H. R Maidana ◽  
W. R. Cabrera ◽  
A. D. Báez ◽  
R. M. Ledesma
Keyword(s):  
El Paso ◽  
Lutzomyia Longipalpis ◽  
Lutzomyia Migonei

El flebótomo Lutzomyia longipalpis es, hasta el momento, el vector comprobado de la leishmaniosis visceral canina. Capturas realizadas en Corrientes (Argentina) permitieron identificar a Lutzomyia neivai, vector de la leishmaniosis cutánea, así como a Lutzomyia migonei y Lutzomyia cortelezzii-sallesi, ambos considerados vectores secundarios de la leishmaniosis cutánea. Para el control epidemiológico resulta primordial la identificación del vector. Luego de la captura de los insectos, el paso más complicado es el reconocimiento de las distintas especies de Lutzomyia, para lo cual es necesario lograr el aclaramiento de sus estructuras y observarlo al microscopio prestando atención a los descriptores característicos y particulares de la especie. Existen aproximadamente 30 descriptores para identificar Lutzomyia longipalpis por la observación microscópica de su aparato genital masculino. El objetivo de este trabajo fue seleccionar cinco descriptores de fácil identificación, que en orden de importancia resultaron ser: setae diferenciadas del parámero, relación entre longitud del parámero y lóbulo lateral, forma y apariencia del coxito o basistilo, características y ubicación del mechón de setae del coxito y morfología de la bomba eyaculadora. La sencilla visualización de estos descriptores permite una rápida identificación taxonómica de la especie.

scholarly journals Deltamethrin-impregnated dog collars have a potent anti-feeding and insecticidal effect on Lutzomyia longipalpis and Lutzomyia migonei

2001 ◽  
Vol 96 (6) ◽  
pp. 839-847 ◽  
Author(s):  
John R David ◽  
Luisa M Stamm ◽  
Haroldo Sergio Bezerra ◽  
Raimundo Nonato Souza ◽  
Robert Killick-Kendrick ◽  
...  
Keyword(s):  
Lutzomyia Longipalpis ◽  
Insecticidal Effect ◽  
Lutzomyia Migonei

Saliva of laboratory-reared Lutzomyia longipalpis exacerbates Leishmania (Leishmania) amazonensis infection more potently than saliva of wild-caught Lutzomyia longipalpis

2009 ◽  
Vol 58 (3) ◽  
pp. 220-226 ◽  
Author(s):  
Márcia Dalastra Laurenti ◽  
Veruska Marques dos Santos Silveira ◽  
Nágila Francinete Costa Secundino ◽  
Carlos Eduardo Pereira Corbett ◽  
Paulo Paulocci Filemon Pimenta
Keyword(s):  
Leishmania Amazonensis ◽  
Lutzomyia Longipalpis

scholarly journals Abortive infection of Lutzomyia longipalpis insect vectors by aflagellated LdARL-3A-Q70L overexpressing Leishmania amazonensis parasites

2003 ◽  
Vol 5 (10) ◽  
pp. 717-728 ◽  
Author(s):  
Armelle Cuvillier ◽  
Jose Carlos Miranda ◽  
Audrey Ambit ◽  
Aldina Barral ◽  
Gilles Merlin
Keyword(s):  
Leishmania Amazonensis ◽  
Lutzomyia Longipalpis ◽  
Insect Vectors ◽  
Abortive Infection

scholarly journals Sand fly captures with Disney traps in area of occurrence of Leishmania (Leishmania) amazonensis in the state of Mato Grosso do Sul, mid-western Brazil

2010 ◽  
Vol 43 (5) ◽  
pp. 491-495 ◽  
Author(s):  
Maria Elizabeth Cavalheiros Dorval ◽  
Tulia Peixoto Alves ◽  
Geucira Cristaldo ◽  
Hilda Carlos da Rocha ◽  
Murilo Andrade Alves ◽  
...  
Keyword(s):  
Natural Infection ◽  
Leishmania Species ◽  
Leishmania Amazonensis ◽  
Sand Fly ◽  
Lutzomyia Longipalpis ◽  
Mato Grosso ◽  
Predominant Species ◽  
American Cutaneous Leishmaniasis ◽  
Phlebotomine Sandflies ◽  
Mato Grosso Do Sul

INTRODUCTION: The work was conducted to study phlebotomine fauna (Diptera: Psychodidae) and aspects of American cutaneous leishmaniasis transmission in a forested area where Leishmania (Leishmania) amazonensis occurs, situated in the municipality of Bela Vista, State of Mato Grosso do Sul, Brazil. METHODS: The captures were conducted with modified Disney traps, using hamster (Mesocricetus auratus) as bait, from May 2004 to January 2006. RESULTS: Ten species of phlebotomine sandflies were captured: Brumptomyia avellari, Brumptomyia brumpti, Bichromomyia flaviscutellata, Evandromyia bourrouli, Evandromyia lenti, Lutzomyia longipalpis, Psathyromyia campograndensis, Psathyromyia punctigeniculata, Psathyromyia shannoni and Sciopemyia sordellii. The two predominant species were Ev bourrouli (57.3%) and Bi flaviscutellata (41.4%), present at all sampling sites. Two of the 36 hamsters used as bait presented natural infection with Leishmania. The parasite was identified as Leishmania (Leishmania) amazonensis. CONCLUSIONS: Analysis of the results revealed the efficiency of Disney traps for capturing Bichromomyia flaviscutellata and the simultaneous presence of both vector and the Leishmania species transmitted by the same can be considered a predictive factor of the occurrence of leishmaniasis outbreaks for the human population that occupies the location.

scholarly journals Evidência de transmissão de leishmaniose visceral por Lutzomyia cruzi no município de Jaciara, Estado de Mato Grosso, Brasil

2011 ◽  
Vol 44 (1) ◽  
pp. 76-78 ◽  
Author(s):  
Nanci Akemi Missawa ◽  
Márcia Aurélia Esser Veloso ◽  
Giovana Belem Moreira Lima Maciel ◽  
Érika Monteiro Michalsky ◽  
Edelberto Santos Dias
Keyword(s):  
Leishmania Infantum ◽  
Leishmania Amazonensis ◽  
Lutzomyia Longipalpis ◽  
Mato Grosso ◽  
Leishmania Infantum Chagasi ◽  
Dna Pcr

INTRODUÇÃO: O município de Jaciara foi classificado em 2003, como área de transmissão de leishmaniose visceral em situação de surto. O trabalho objetivou determinar evidência de transmissão de Leishmania (Leishmania) infantum chagasi por Lutzomyia cruzi no município de Jaciara, Estado de Mato Grosso, Brasil. MÉTODOS: O município situa-se a 127km da capital Cuiabá e é um importante ponto de atração para os praticantes de eco-turismo. Fêmeas de Lutzomyia cruzi, capturadas com armadilha de CDC, foram dissecadas para confirmação da espécie e armazenadas a -20ºC em pools de 10 indivíduos para extração de DNA, PCR genérico, RFLP específico e eletroforese em gel de poliacrilamida. RESULTADOS: O levantamento entomológico demonstrou a ocorrência abundante de Lutzomyia cruzi e ausência de Lutzomyia longipalpis, principal vetora da Leishmania (Leishmania) infantum chagasi. Uma das três amostras analisadas apresentou banda característica de DNA de Leishmania (120pb) em PCR genérico. Para confirmação da espécie de Leishmania, na RFLP utilizaram-se controles positivos de Leishmania (Leishmania) amazonensis, Leishmania (Viannia) braziliensis e Leishmania (Leishmania) infantum chagasi digeridas com enzima de restrição HaeIII. Constatou-se um padrão de bandas semelhante à Leishmania (Leishmania) infantum chagasi em uma amostra, confirmando a detecção de infecção natural de Leishmania (Leishmania) infantum chagasi em Lutzomyia cruzi. CONCLUSÕES: A ocorrência de casos humanos e cães positivos, a presença da Lutzomyia cruzi e a ausência de Lutzomyia longipalpis, bem como a detecção de infecção natural por Leishmania (Leishmania) infantum chagasi, evidenciam a participação de Lutzomyia cruzi na transmissão da leishmaniose visceral em Jaciara, Estado de Mato Grosso, Brasil.

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